Miscellaneous GNB Flashcards
Francisella disease assocation and gov’t status
- tularemia
- potential bioterrorism agent
Francisella transmitted by ____
wild animals such as deer, rabbits, beavers, and squirrels
How do humans get tularemia?
- skinning animals
- undercooked game
- animal/tick bites
Francisella biosafety level
BSL3
F. tularensis signs/symptoms
- skin ulcers
- infection of lymph nodes/eyes/lungs/GIT
Francisella gram stain morphology
faintly staining coccobacilli
Francisella medium of choice
glucose-cystine blood agar
Francisella colony morphology
small and grayish
Francisella testing
- agglutination
- direct fluorescent antibody tests
Brucella disease association
Brucellosis aka undulant fever
How is Brucella acquired?
drinking contaminated milk or slaughter house exposure
List the 4 Brucella spp that infect humans
- B. melitenis
- B. abortus
- B. suis
- B. canis
Brucella gov’t status and biosafety level
potential bioterrorism agent
BSL3
Brucella media and incubation requirements
- fastidious
- Brucella buffered charcoal yeast extract (BCYE)
- Modified Thayer Martin (MTM)
- 10% CO2 humid air
Brucella biochemical traits
oxygen requirement
oxidase
catalase
- strict aerobe
- oxidase pos
- catalase pos
Bordetella pertussis disease association
Pertussis aka whooping cough
Bordetella inhabits which body part(s)?
Mucous membranes of the respiratory tract
3 pertussis stages
- Catarrhal = general flulike symptoms
- Paroxysmal = repetitive coughing episodes
- Convalescent = recovery phase
B. pertussis media requirements
- Bordet-Gengou (potato infusion)
- Regan-Lowe (charcoal horse blood agar)
- add cephalexin to select
Other 2 Bordetella spp
- B. parapertussis = mild resp. infection in humans
- B. bronchiseptic = kennel cough in dogs and infrequently infects humanas
B. pertussis colony morphology
- small/smooth
- mercury droplets
- beta hemolytic
B. pertussis gram morphology
- poorly stained coccobacilli
- single or in pairs
B. pertussis biochemical characteristics
MAC growth
urease
- does not grow on MAC
- urease neg
other Bordetella spp grow on MAC and are urease pos
Actinobacillus spp normal environment
oral flora of animals
Which Actinobacillus spp is normal flora of humans?
A. actinomycetmcomitans
Actinobacillus infections caused by what?
Animal bites
A. actinomycetomcomitans disease association
- endocarditis
- gum disease
A. actinomycetomcomitans media requirements
- grows on SBA and CHOC
- does not grow on MAC
A. actinomycetocomitans colony morphology
star-like centers