Non-Fermenting Bacteria Flashcards
recovered in sewage, sludge, soil
Stenotrophomonas maltophilia
Lavender to lavender
Green on BAP
Stenotrophomonas maltophilia
Determines bacteria’s ability to ferment or oxidize CHO
Oxidation Fermentation Medium of Hugh and Leifson
can be mistaken for Neisseria
Acinetobacter
causes Melioidosis or Vietnam Time Bomb
Burkholderia pseudomallei
Rice water stools
Sunken eyes
Washerwoman’s hand skin
Cholera
Cetrimide Agar (+) Acetamide utilization (+)
Pseudomonas aeruginosa
Alcaligenes that is:
• peritrichous
• asaccharolytic
Alcaligenes faecalis
JUST penicillin resistant
Acinetobacter
2nd most common species implicated in gastroenteritis
Vibrio parahaemolyticus
Cholera:
responsible toxin
heat-labile
stimulates adenylate cyclase
Choleragen
Gastric non-hodgkin’s lymphoma
Helicobacter pylori
Expresses A, B, and C
Hikojima
aggressive granulomatous pulmonary disease with metastatic abscess in lungs caused by ingestion/inhalation/inoculation of the organism
Burkholderia mallei
Acinetobacter lwoffii’s other name
Asaccharolytic Acinetobacter
sources from water and muddy soils
Burkholderia pseudomallei
Stenotrophomonas maltophilia formerly known as
Pseudomonas maltophilia
Xanthomonadaceae:
Stenotrophomonas maltophilia
differentiates nitrate reducing strains of A . lwoffii from CDC Group 2
Acinetobacter transformation test
Yellow pigmented Pseudomonas that has a single polar flagella
Pseudomonas oryzihabitans
BAP: feathered edge colonies surrounded by a green discoloration zone
Alcaligenes faecalis
Almost all strains are resistant to: •Penicillin •Cephalosporins •Ampicillin •Cefuroxime
Pseudomonas
Purple on MAC
Acinetobacter
non-oxidizer
less virulent
contaminant
lactose (-)
Acinetobacter lwoffii
BHI Agar with Tyrosine: brown pigment
BAP: large smooth glistening colonies with uneven edges
Stenotrophomonas maltophilia
Wrinkled colonies
Pseudomonas stutzeri
Pseudomonas oryzihabitans
Burkholderia pseduomallei
Newborns: meningitis/septicemia
Elizabethkingia meningoseptica
Sensitive to O2 and sulfur oxide;
thermophilic
Campylobacter jejuni
Burkholderia pseudomallei’s odor
Earthy odor
pH indicator of OF medium
bromothymol blue
nosocomial infections involving respiratory tract
Pseudomonas aeruginosa
Violet
Chromobacterium violaceum
Motile
O. urethralis or O. ureolytica
O. ureolytica
Virulence factor: survive within phagocytes
Burkholderia pseudomallei
Give yellow pigmenters
Chryseobacterium Elizabethkingia Pseudomonas luteola Pseudomonas oryzihabitans Pseudomonas stutzeri Sphingobacterium
most common cause of gastroenteritis worldwide
Campylobacter jejuni subsp. jejuni
Motile with a single polar flagellum
broth media: sheathed
solid media: unsheathed
Vibrio
causes animal infections (zoonotic) called glanders/farcy
Burkholderia mallei
High mortality among debilitated patients and immunocompromised
Stenotrophomonas maltophilia
Ecthyma gangrenosum
cutaneous papules that become black and necrotic
Ammonia like odor
Cornflower blue color
Acinetobacter baumannii
A. lwoffii’s agar
Acinetobacter minimal agar
Grapelike or tortilla-like;
some smell like rotten potatoes
Pseudomonas aeruginosa
Antibiotic resistance:
MDR/XDR
AmpC beta lactamases
Porin channels mutation
Acinetobacter
Flavobacteriaceae:
Elizabethkingia
Chyrseobacterium
Spingomonas
type of Pseudomonas that are soil denitrifiers
Nun-fluorescent group
- Pseudomonas stutzeri
- Pseudomonas mendocina
T or F:
Burkholderia pseudomallei has large rods with bipolar staining in their Gram stain
False
*small rods
Expresses A and B antigen
Ogawa
BAP: pale yellow colonies
Elizabethkingia meningoseptica
Kanagawa phenomenon
V. parahaemolyticus
Pseudomonas that produce yellow non-diffusible pigment
Yellow Pigmented Group
•Pseudomonas luteolus
• Pseudomonas oryzihabitans
Environmental organism found in blood and sputum of CF patients
Ralstonia pickettii
Expresses A and C antigen
Inaba
Jacuzzi/Hot Tub Syndrome
Pseudomonas aeruginosa
Motility: darting/shooting star motility
Vibrio
can cause local suppurative or acute pulmonary infection
Burkholderia mallei
Pseudomonas that grow at 4 degrees Celsius
Pseudomonas fluorescens
Pseudomonas putida
Alcaligenes faecalis also known as
Acinetobacter odorans
3rd most commonly isolated non-fermenter in clinical laboratories
Stenotrophomonas maltophilia
Intrinsically resistant to most:
• beta lactams
• aminoglycosides
Stenotrophomonas maltophilia
Kommabacillus
Vibrio cholerae
Has the ff virulence factors:
• capsule (alginate)
• exotoxin A
• Quorum sensing
Pseudomonas aeruginosa
Ammonia-like odor
glucose and maltose oxidizer
ONPG (+)
Stenotrophomonas maltophilia
Acinetobacter was known as
Mima
undergoes coccal formation and fragile
Helicobacter pylori
Pseudomonas aeruginosa agars that enhance pigment production
Flo Agar
Pseudomonas F Agar
Tech Agar
Pseudomonas P Agar
Has a bluish or peach pink tint on MAC
Acinetobacter
Predominant Achrombacter species isolated from CF patients around the world
Achromobacter xylocidians
Pink
Roseomonas (pink mucoid)
Methylobacterium (pink to coral)
Oligella urethralis was formerly known as
Moraxella urethralis
Yellow pigmented Pseudomonas that has multitrichous flagella
Pseudomonas luteolus
Vibriostatic agent 0/129
S or R?
Susceptible
H2S (+)
Shewanella putrefaciens
Give Oxidase (-) bacilli
Pseduomonas luteola Pseudomonas oryzihabitans Burkholderia cepacia Acinetobacter Stenotrophomonas maltophilia
P2BAS
CANNOT grow in Jordan’s Tartrate
Alcaligenes fecalis
Strain: O3:K6
Vibrio parahaemolyticus
Water-loving
Aeromonas
Bengal Strain
O1 and O139
Acinetobacter baumannii’s other name
Saccharolytic Acinetobacter
Pseudomonas aeruginosa has a fruity odor due to?
2-aminoacetophenone
CAN grow in Jordan’s Tartrate
Malonate (+)
Alcaligenes/Achromobacter denitrificans
frequently isolated species
Pseudomonas aeruginosa
affects livestock
Burkholderia mallei
Blue-green
Pseudomonas aeruginosa
Sweet odor
Alcaligenes faecalis (apples and pears)
Myroides odoratus (popcorn)
EO-4
Neisseria zoodegmatis
*AMEN
Red leg disease in frogs
Aeromonas
Increased severity among patients with CF and CGD
Burkholderia cepacia
BAP: dark yellow colonies
Chryseobacterium indologenes
Burkholderia cepacia’s agar?
Burkholderia cepacia selective agar
H2S producer in TSI;
wound, ear infections
Shewanella putrefaciens
Gold standard for diagnosis of H. pylori gastritis
Histologic Staining and Culture of Biopsy (grind sample)
blue green exudate and corneal ulcers infection
Keratitis
Pseudomonas aeruginosa
Swimmer’s/Diver’s ear
Pseudomonas aeruginosa
Achromobacter denitrificans formerly known as
Alcaligenes denitrificans
Non-motile
Moraxella Acinetobacter Chryseobacterium Elizabethkingia Sphingobacterium Oligella
*MACESO
Burkholderia pseudomallei uses what medium?
Ashdown Medium
2nd most commonly isolated non-fermenter in the laboratory
Acinetobacter baumannii
causes 2nd most serious disease
Vibrio vulnificus
important pathogen among immunocompromised
Pseudomonas aeruginosa
least pathogenic vibrio
Vibrio alginolyticus
Plant pathogen causing onion bulb rot and foot rot
Burkholderia cepacia
T or F: V. fluvialis and V. furnissii are lactose fermenters.
False
*sucrose