Haemophilus and Other Fastidious Bacteria Flashcards
blood lover
Gr (-), small, pleomorphic coccobacilli
Haemophilus
most require X (hemin) and V (NAD) factors except H. aphrophilus
Haemophilus
has satellite growth
Haemophilus
Pfeiffer’s Bacillus
Haemophilus influenzae
major pathogen and frequently associated during flu pandemics
Haemophilus influenzae
has the ff virulence factors:
- capsule (serotyping)
- IgA protease
- fimbriae/pili
- outer membrane proteins and LPS
Haemophilus influenzae
Ducrey’s Bacillus
Haemophilus ducreyi
strict human pathogen;
female sex workers
Haemophilus ducreyi
causes Chancroid or Soft Sore
Haemophilus ducreyi
Gram stain looks similar to a school of fish
Haemophilus ducreyi
Haemophilus influenzae biogroup III;
- non-encapsulated
- Pediatric conjuctivitis
- Brazillian purpuric fever
Haemophilus influenzae biogroup aegyptius
Koch-Weeks Bacillus
Haemophilus aegyptius
causes pink eye (highly contagious conjuctivitis)
Haemophilus aegyptius
What staining is done to diagnose Haemophilus?
Acridine orange staining
Streaking done for primary isolation and satellite growth
Staph streak
Nairobi plate is for
H. ducreyi
It is a clear medium with X and V factors
Levinthal Agar
Delta-aminolevulinic acid test is also known as
Porphyrin test
The porphyrin test’s (+) result is
red fluorescence (brick red)
susceptible to penicillin and has a bipolar staining of sefety pin appearance
Pasteurella multocida
has a mushroom smell/musty odor
Pasteurella multocida
Shipping Fever in cattles
Pasteurellosis
Bang’s Bacillus
Brucella
Gram (-) bacilli/coccobacilli has sand appearance
Brucella
It induces spontaneous abortions and sytemic disease
Brucella
requires niacin for growth
Brucella abortus
It is the gold standard for Brucella
Serum Agglutination Test
(detection of febrile agglutinins)
Facultative intracellular pathogen.
Causative agent of Tularemia
(Rabbit, Deerfly, Lemming, Water Rat Tapper’s fevers)
Francisella tularensis
signs & symptoms include:
- ulceroglandular (most common; ulcers)
- pneumonic (most serious form)
- oculoglandular (conjuctivitis and lymphadenopathy)
- typhoidal (no skin lesions)
Francisella tularensis
causes waterborne tularemia and less virulent
Francisella philomiragia
Bordet Gengou Bacillus;
capsulated;
DTaP (Diphtheria, Tetanus, Pertussis)
Bordetella pertussis
causative agent of whooping cough or pertussis
(highly contagious)
Bordetella pertussis
causes kennel cough among animals
Bordetella bronchiseptica
petussis-like illness
Bordetella holmesii
preferred staining for Bordetella
Direct Fluorescent Antibody stain
(+) yellow green fluorescence
Bordetella pertussis is incubated at?
35 degrees Celsius for 7 days in
Bordet Gengou Potato Infusion Agar
all are sodium hippurate hydrolysis
(except L. pneumophila)
Legionella
no recorded/documented person to person infection
Legionella
most clinically significant species;
possess 2 well-defined, morphologically distinct forms in HeLa cells
Legionella pneumophila
Pontiac fever is associated with
Legionnaires disease
causes Pittsburgh pneumonia
Legionella micdadei
causes Wiga agent pneumonia
Legionella bozemanii
causes pneumonia
Legionella dumoffi
sputum is treated with ____ sol’n
KCl:HCl
What is HACEK?
_H_aemophilus spp.
_A_ggregatibacter
_C_ardiobacterium hominis
_E_ikenella corrodens
_K_ingella kingae
loves high levels of CO2;
does not require X and V factor
Aggregatibacter aphrophilus
lactose fermenters that has a grade school paste odor
Aggregatibacter aphrophilus
presence of a star shape with 4-6 points in the center of colonies growing in BHI agar after 48 hrs
Aggregatibacter actinomycetemcomitans
virulence factors:
- collagenase
- leukotoxin
Aggregatibacter actinomycetemcomitans
has a rosette formation, teardrop, dumbell, lollipop appearance
Cardiobacterium hominis
capnophilic that has a bleach-like odor
Eikenella corrodens
B-hemolytic on BAP
catalase (-)
indole (-)
Kingella kingae
can be mistaken for N. gonorrhoeae on Modified Thayer Martin Agar
Kingella denitrificans
