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Bacteriology > Culture Media in Diagnostic Microbiology (51-104) > Flashcards

Culture Media in Diagnostic Microbiology (51-104) Flashcards

1
Q

Selective and Differential

isolation and differentiation of lactose and non-lactose fermenting Gr (-) bacilli

A

MacConkey Agar

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2
Q

Selective and Differential

isolation and differential of mannitol and non-mannitol fermenting Staphylococci

A

Mannitol Salt Agar (MSA)

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3
Q

Selective

isolation of pathogenic Neisseria sps. like N. gonorrhoeae

A

Martin Lewis Agar

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4
Q

Selective

isolation of Listeria monocytogenes

A

McBride Medium

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5
Q

Selective

isolation of Mycobacterium species

A 
Middlebrook 7H9 (liquid)
7H10/7H11 (solid)
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6
Q

Selective

isolation of Mycobacterium species

A

Mitchison Agar

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7
Q

Antibiotic Susceptibility Test Medium

AST of most bacteria

A

Mueller-Hinton Agar/broth

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8
Q

Tissue Cell Culture

isolation of Chlamydia

A

McCoy cells

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9
Q

Biochemical Characteristic Medium

identification of Clostridium perfringes

A

Litmus Milk Medium

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10
Q

Biochemical Characteristic Medium

identification of Gr (-) enteric bacilli based on ability to produce LDC/ODC/ADH

A

Moeller’s Broth

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11
Q

Biochemical Characteristic Medium

differentiation of organisms based on their ability to oxidize or ferment carbohydrates

A

Oxidation-Fermentation (O-F) medium

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12
Q

Selective

isolation of Neisseria spp.

A

Modified Thayer Martin Medium

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13
Q

Selective

isolation of pathogenic sps. of Neisseria like N. gonorrhoeae and also Mycoplasma hominis and Ureaplasma urealyticum

A

New York City Agar

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14
Q

Selective

isolation of Mycobacterium spp.

A

Dorset Egg Medium

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15
Q

Selective

isolation of Mycobacterium spp.

A

Petragnani Medium

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16
Q

Selective

isolation of Mycobacterium spp.

A

American Thoracic Society Medium

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17
Q

Selective

isolation of non tuberculous Mycobacteria like M. avium

A

Wallenstein Medium

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18
Q

Selective

selective isolation of gram (+) cocci and anaerobic gram (-) bacilli

A

Phenylethanol Agar

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19
Q

Selective and Enrichment

isolation of Bordetella pertussis

A

Regan Lowe Agar

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20
Q

Selective and Differential

isolation and differentiation of Salmonella and Shigella

A

Salmonella-Shigella Agar

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21
Q

Nonselective Medium

recovery of aerobes and anaerobes

A

Schaedler Agar

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22
Q

Enrichment

enrichment and isolation of Salmonella spp.

A

Selenite F Broth

23
Q

Selective

isolation of Campylobacter spp.

A

Skirrow’s Medium

24
Q

Selective

isolation of S. pyogenes and S. agalactiae

A

Streptococcal Selective Agar

25
Q

Differential

differentiating non-fermentative bacilli like Pseudomonas, Acinetobacter, Alkaligenes

A

Seller’s Agar

26
Q

Biphasic Medium

blood culture

A

Scott’s Modified Castañeda and Thioglycollate Broth

27
Q

Transport

maintenance of viability of microorganisms

A

Stuart’s Medium

28
Q

Selective

isolation of Mycoplasma spp.

A

Shepard’s A7-B Agar

29
Q

Enrichment

isolation of Salmonella and Shigella spp from stools

A

Tetrathionate Broth

30
Q

Selective

isolation of pathogenic spp of Neisseria

A

Thayer Martin Agar

31
Q

General Supportive

recovery of aerobes, anaerobes, microaerophilic, and fastidious microorganisms

A

Thioglycollate Broth

32
Q

Selective

isolation of Corynebacterium diphtheriae

A

Tinsdale Agar

33
Q

Selective and Differential

isolation and differentiation of Vibrio spp.

A

Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS)

34
Q

Enrichment and Selective

enrichment and isolation of Streptococcus agalactiae

A

Todd-Hewitt Broth supplemented with antibiotics

35
Q

General Purpose Medium

supports the growth of non-fastidious and fastidious bacteria;

can be used as a base in preparing blood agar

A

Trypticase Soy Agar/Broth

36
Q

Selective Transport

Neisseria gonorrhoeae

A

Transgrow

37
Q

Biochemical Characteristic Medium

identification of Gr (-) enteric bacilli based on their ability to produce indole

A

Tryptone Broth

38
Q

Biochemical Characteristic Medium

identification of Gr (-) enteric bacilli based on their ability to
(1) ferment glucose, lactose, and sucrose; (2) produce gas and hydrogen sulfide

A

Triple Sugar Iron Agar

39
Q

Biochemical Characteristic Medium

identification of Gr (-) enteric bacilli based on their ability to ferment glucose via mixed acid fermentation or butylene glycol pathway

A

Methyl Red Voges Proskauer Broth (MRVP) or

Peptone Glucose Broth

40
Q

Biochemical Characteristic Medium

identification of Gr (-) enteric bacilli based on their ability to produce enzyme urease

A

Christensen Urea Agar or

Stuart Urea Broth

41
Q

Biochemical Characteristic Medium

identification of Gr (-) enteric bacilli based on their ability to produce enzyme deaminase

A

Phenylalanine Deaminase Agar (PDA)

42
Q

Biochemical Characteristic Medium

identification if Gr (-) enteric bacilli based on their ability to utilize citrate as source of carbon

A

Simmon’s Citrate Agar

43
Q

Differential Medium

identification of organisms based in their ability to grow or not to grow in the presence of 6.5% NaCl

A

Salt (NaCl) Broth

44
Q

Biochemical Characteristic Medium

differentiate beta-hemolytic streptococci and enterococci based on their ability to produce enzyme, pyrrolidonylarylamidase or pyroglutamylaminopeptidase

A

PYR Agar

45
Q

Selective

isolate and identify Pseudomonas aeruginosa

A

Cetrimide Agar Slant

46
Q

Biochemical Characteristic Medium

identification of organisms which utilize acetate as a source of carbon like Escherichia coli

A

Acetate Slant

47
Q

Biochemical Characteristic Medium

identification of organisms which utilize acetamide as a source of carbon like P. aeruginosa

A

Acetamide Slant

48
Q

Selective and Differential

identification of Gr (-) enteric bacilli and for the differentiation of Salmonella and Shigella spp.

A

Xylose Lysine Desoxycholate Agar

49
Q

Selective

identification of Corynebacterium diphtheriae

A

Pai’s Agar

50
Q

Selective

identification of Corynebacterium diphtheriae

A

McLeod’s Agar

51
Q

Tissue Cell Culture

isolation of Rickettsia

A

HeLa Cell Line

52
Q

Tissue Cell Culture

isolation of Rickettsia

A

Embryonated Egg

53
Q

Employed in Animal Inoculation

isolation and identification of Mycobacterium leprae

A

Foot Pad of Mice and Armadillo

Bacteriology (11 decks)

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