Culture Media in Diagnostic Microbiology (51-104) Flashcards
Selective and Differential
isolation and differentiation of lactose and non-lactose fermenting Gr (-) bacilli
MacConkey Agar
Selective and Differential
isolation and differential of mannitol and non-mannitol fermenting Staphylococci
Mannitol Salt Agar (MSA)
Selective
isolation of pathogenic Neisseria sps. like N. gonorrhoeae
Martin Lewis Agar
Selective
isolation of Listeria monocytogenes
McBride Medium
Selective
isolation of Mycobacterium species
Middlebrook 7H9 (liquid) 7H10/7H11 (solid)
Selective
isolation of Mycobacterium species
Mitchison Agar
Antibiotic Susceptibility Test Medium
AST of most bacteria
Mueller-Hinton Agar/broth
Tissue Cell Culture
isolation of Chlamydia
McCoy cells
Biochemical Characteristic Medium
identification of Clostridium perfringes
Litmus Milk Medium
Biochemical Characteristic Medium
identification of Gr (-) enteric bacilli based on ability to produce LDC/ODC/ADH
Moeller’s Broth
Biochemical Characteristic Medium
differentiation of organisms based on their ability to oxidize or ferment carbohydrates
Oxidation-Fermentation (O-F) medium
Selective
isolation of Neisseria spp.
Modified Thayer Martin Medium
Selective
isolation of pathogenic sps. of Neisseria like N. gonorrhoeae and also Mycoplasma hominis and Ureaplasma urealyticum
New York City Agar
Selective
isolation of Mycobacterium spp.
Dorset Egg Medium
Selective
isolation of Mycobacterium spp.
Petragnani Medium
Selective
isolation of Mycobacterium spp.
American Thoracic Society Medium
Selective
isolation of non tuberculous Mycobacteria like M. avium
Wallenstein Medium
Selective
selective isolation of gram (+) cocci and anaerobic gram (-) bacilli
Phenylethanol Agar
Selective and Enrichment
isolation of Bordetella pertussis
Regan Lowe Agar
Selective and Differential
isolation and differentiation of Salmonella and Shigella
Salmonella-Shigella Agar
Nonselective Medium
recovery of aerobes and anaerobes
Schaedler Agar
Enrichment
enrichment and isolation of Salmonella spp.
Selenite F Broth
Selective
isolation of Campylobacter spp.
Skirrow’s Medium
Selective
isolation of S. pyogenes and S. agalactiae
Streptococcal Selective Agar
Differential
differentiating non-fermentative bacilli like Pseudomonas, Acinetobacter, Alkaligenes
Seller’s Agar
Biphasic Medium
blood culture
Scott’s Modified Castañeda and Thioglycollate Broth
Transport
maintenance of viability of microorganisms
Stuart’s Medium
Selective
isolation of Mycoplasma spp.
Shepard’s A7-B Agar
Enrichment
isolation of Salmonella and Shigella spp from stools
Tetrathionate Broth
Selective
isolation of pathogenic spp of Neisseria
Thayer Martin Agar
General Supportive
recovery of aerobes, anaerobes, microaerophilic, and fastidious microorganisms
Thioglycollate Broth
Selective
isolation of Corynebacterium diphtheriae
Tinsdale Agar
Selective and Differential
isolation and differentiation of Vibrio spp.
Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS)
Enrichment and Selective
enrichment and isolation of Streptococcus agalactiae
Todd-Hewitt Broth supplemented with antibiotics
General Purpose Medium
supports the growth of non-fastidious and fastidious bacteria;
can be used as a base in preparing blood agar
Trypticase Soy Agar/Broth
Selective Transport
Neisseria gonorrhoeae
Transgrow
Biochemical Characteristic Medium
identification of Gr (-) enteric bacilli based on their ability to produce indole
Tryptone Broth
Biochemical Characteristic Medium
identification of Gr (-) enteric bacilli based on their ability to
(1) ferment glucose, lactose, and sucrose; (2) produce gas and hydrogen sulfide
Triple Sugar Iron Agar
Biochemical Characteristic Medium
identification of Gr (-) enteric bacilli based on their ability to ferment glucose via mixed acid fermentation or butylene glycol pathway
Methyl Red Voges Proskauer Broth (MRVP) or
Peptone Glucose Broth
Biochemical Characteristic Medium
identification of Gr (-) enteric bacilli based on their ability to produce enzyme urease
Christensen Urea Agar or
Stuart Urea Broth
Biochemical Characteristic Medium
identification of Gr (-) enteric bacilli based on their ability to produce enzyme deaminase
Phenylalanine Deaminase Agar (PDA)
Biochemical Characteristic Medium
identification if Gr (-) enteric bacilli based on their ability to utilize citrate as source of carbon
Simmon’s Citrate Agar
Differential Medium
identification of organisms based in their ability to grow or not to grow in the presence of 6.5% NaCl
Salt (NaCl) Broth
Biochemical Characteristic Medium
differentiate beta-hemolytic streptococci and enterococci based on their ability to produce enzyme, pyrrolidonylarylamidase or pyroglutamylaminopeptidase
PYR Agar
Selective
isolate and identify Pseudomonas aeruginosa
Cetrimide Agar Slant
Biochemical Characteristic Medium
identification of organisms which utilize acetate as a source of carbon like Escherichia coli
Acetate Slant
Biochemical Characteristic Medium
identification of organisms which utilize acetamide as a source of carbon like P. aeruginosa
Acetamide Slant
Selective and Differential
identification of Gr (-) enteric bacilli and for the differentiation of Salmonella and Shigella spp.
Xylose Lysine Desoxycholate Agar
Selective
identification of Corynebacterium diphtheriae
Pai’s Agar
Selective
identification of Corynebacterium diphtheriae
McLeod’s Agar
Tissue Cell Culture
isolation of Rickettsia
HeLa Cell Line
Tissue Cell Culture
isolation of Rickettsia
Embryonated Egg
Employed in Animal Inoculation
isolation and identification of Mycobacterium leprae
Foot Pad of Mice and Armadillo
