Gr (+) Bacilli Flashcards
Klebs-Loeffler bacillus
(isolation: Klebs
discovery: Loeffler)
Corynebacterium
may appear as club-shaped/Chinese character/picket fence (diphtheroid)
Corynebacterium
possess Babes-Ernts Granules (metachromatic granules)
Corynebacterium diphtheriae
Bull’s neck appearance
causative agent of Diphtheria
Corynebacterium diphtheriae
give the 4 biotypes of Corynebacterium diphtheriae
- largest colony type
- fried-egg apperance
- small colonies in BAP & black colonies with gray on Tellurite medium
- looks like mitisa and rarely carried diphtheriae toxic gene
- gravis
- mitis
- intermedius
- belfanti
resistant to many antibiotics;
mastitis
Corynebacterium jeikeium
Gold standard for diphtheria toxin production
Vero Cell Toxicity Assay
Shick’s test (diptheria) (+) result
redness
*skin test
has a tumbling motility and able to grow at 4 degrees Celsius
Listeria monocytogenes
virulence factors:
- Listeriolysin O
- Act A
- Siderophores
- Internalins
Listeria monocytogenes
What is L. monocytogenes’s growth appearance on semi-solid medium?
(Hint: There are 2 descriptions.)
- inverted Christmas tree
- inverted umbrella
L. monocytogenes’ test for pathogenicity?
Ocular test for Anton
normal flora of urogenital tract causes bacterial vaginosis
Gardnerella vaginalis
presence of clue cells
(vaginal epithelial cell through which the orgnism touches
the border of the epithelial cell)
Gardnerella vaginalis
Test for Gardnerella vaginalis
Whiff Test (+): fishy amine odor
