NON - FERMENTATIVE GRAM (-) BACILLI Flashcards
NON - FERMENTATIVE GRAM (-) BACILLI
OF medium
Pseudomonas spp that grow at 35 and 42 degC
P. aeruginosa
Grape-like or corn-taco like odor
Swimmer’s ear
#2 cause of burn infection
#1 in ICU isolate
#1 in cystic fibrosis
#3 in nosocomial infection
P. aeruginosa
P. aeruginosa
- ____ encapsulated and ____ spore former
- In BAP-
- Catalase, Arginine dihydrolase Acetamide
Utilization Citrate
Non; non
ground glass appearance
(+)
P. aeruginosa
Virulence factors:
Exo and endotoxin
P. aeruginosa on Cetrimide medium
“Jacuzzi or hot tub syndrome” or Shanghai fever
P. aeruginosa
Pigment:
Pyocyanin-common, bluish
Fluorescent Group in Pseudomonas (AFPV)
P. aeruginosa
P. fluorescens
P. putida
P. veronii
Wrinkled colony
NLF
Located at stagnant water
They can able to use ammonium Soil-detrifier
P. stutzeri
Motile, associated to septicemia
No growth at 42 degC
P. putida
Motile
cause UTI, wound infection, bacteremia
No growth at 42 degC
Blood bag contaminant
P. fluorescens
Does not produce pyoverdine
Fat colonies and non wrinkled
Can also use ammonium also soil-detrifier
P. mendocina
Rarely pathogenic organism
Shewanella putrefaciens
Burkholderia
- ___ pathogenic
- ___, ___ spore former, motile EXCEPT
- Catalase and Oxidase
non
Aerobic, non; B. malei
(+)
Only NM, coccobacilli
Cause Glanders disease
MOT: Direct contact
Potential bioterrorism agent
B. mallei
Common Name: Whitmores bacillus
Melioidosis - glanders-like, a severe pneumonia
Vietnamese Time Bomb
Potential bioterrorism agent
Motile
Grow at 42 degC
B. pseudomallei
B. pseudomallei
- Incubation period:
- Bipolar stain:
- Ashdown Culture medium (supplemented by polestin) -
10-20 years
Safety pin appearance
pink to violet colonies
2 cause of cystic fibrosis
Foot rot in man and onion bulb rot in plants
Drug resistant
Earthy or dirt-like odor
B. cepacia
3 most common isolate in NF
Nosocomial-like pneumonia and UTI, NLF
Isolate in medical devices which can cause endocarditis, infection in wounds, bacteremia. Also in frozen foods
Lavender-green pigment
Ammoniacal odor
Growth at 42 degC
Stenotrophomonas maltophila
Maybe mistaken for Neisseria
Medical devices
Cause UTI , wound infection and diarrhea
Acinetobacter calcoaceticus
Acinetobacter calcoaceticus
- ___ motile
- Oxidized ___
- On BAP:
Non
(-)
gummy colonies
High mortality in ventilator ass. pneumoniae
UTIs, pneumonia, septicemia, eye infections
CRAB (Carbapenem-resistant Acinetobacter baumannii)
A. baumanii
Can be mistaken for neisseria and moraxella
Between baumanii and Iwoffi, baumanii is more virulent
Also isolates in medical devices
Ammonia-like odor
A. baumanii
A. baumanii
- Glucose oxidizer -
- EMB:
- ____ to aminoglycosides
- ____ to Penicillin
Yellow
Cornflower blue
Sensitive
Resistant
Non-fermenter
Non-hemolytic ammonia like
A. lwoffi
Beta hemolytic strains
A. haemolyticus
- NLF, coccobacilli
- O. urethralis cause UTI
- O. ureolytica
Oligella
Blepharoconjunctivitis
“pitting of agar”
Moraxella lacunata
NM, Catalase, Oxidase (+)
Gram (-) coccobacilli
CTA Medium
Moraxella osloensis
2nd most common isolated in moraxella
M. nonliquefaciens
- Fruity odor (apples and strawberries)
- Cause eye infection
- Isolate in medical Devices
A. faecalis
A. faecalis
- ___ & Oxidase ___
Motile; (+)
- Can cause neonatal meningitis
- In adult: pneumonia, endocarditis
- Survive in chlorinated water
Elizabethkingia meningoseptica
Elizabethkingia meningoseptica
- Glucose, maltose, mannitol fermenters
and Indole
- ____ & ___ motile
- On BAP & CAP:
(+)
non
yellow pigment
- Resembles vibrios; not a part of normal flora
- Violet pigment
- Almond like odor
- Environmental sources
- Lysine and ornithine
- Growth at 42 degC
Chromobacterium violaceum
