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BACTE finals > ENTEROBACTERIACEAE > Flashcards

ENTEROBACTERIACEAE Flashcards

1
Q

Enterobacteriaceae is a
Gram ___ bacilli
___ anaerobe
___ spore former

A

negative
facultative
non

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2
Q

Enterobacteriaceae is an
Oxidase ___ EXCEPT P. ____
Catalase ___ EXCEPT ____
Xylose fermenters EXCEPT ____
Motile EXCEPT ____, ____, Y. ____
Non-encapsulated EXCEPT ____ & ____
Aerogenic EXCEPT S. ____, ____, ____, ____

A

-; P. shigelloides
+; Shigella (S. dysenteriae)
Shigella
Shigella, Klebsiella, Y. pestis
Klebsiella & Enterobacter
S. typhi, Yersinia, Shigella, Providencia

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3
Q

T/F
Enterobacteriaceae ferments glucose

A

TRUE

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4
Q

TRUE PATHOGENS (3)

A
  1. Lactose Fermenters
  2. Non-Lactose Fermenters
  3. Late Lactose Fermenters
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5
Q

Lactose Fermenters (EKE)

A

Escherichia, Klebsiella, Enterobacter

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6
Q

T/F
Klebsiella and Enterobacter are siblings

A

TRUE

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7
Q

Non-lactose Fermenters

A

All enterobacteriaceae family

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8
Q

Late lactose Fermenters (CAE)

A

Citrobacter, Arizonae, Y. Enterocolitica

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9
Q

ANTIGENS
- K antigen - heat ____
- O antigen - heat ____
- H antigen - heat ____

A

labile
stable
labile

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10
Q
  • 1st genus
  • Common name is Colon bacillus
A

E. coli

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11
Q

E. coli is a normal flora of

A

GIT

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12
Q

E. coli
Predominant ____ anaerobe in ____ and have ____ antigens
Primary virulence factor: ____

A

facultative; Large intestine; 3 (KOH)
PILI

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13
Q

E. coli on BAP

A

BETA hemolytic- Clear of zone hemolysis

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14
Q

T/F
E. coli is mostly motile

A

TRUE

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15
Q
  • # 1 cause of community-acquired UTI
  • # 1 gram-negative SEPSIS
  • # 2 NEONATAL meningitis-nosocomial, BACTEREMIA
A

E. coli

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16
Q

E. coli is ____ in water - fecally contaminated

A

positive

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17
Q

E. coli Serotypes (5)

A
  1. Enteropathogenic E. coli or EPEC
  2. Enteroinvasive E. coli or EIEC
  3. Enteroaggregative E. coli or EAEC
  4. Enterotoxigenic or ETEC
  5. Enterohemorrhagic or EHEC
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18
Q

non bloody diarrhea (no blood, no mucus and no toxins; non invasive, non into toxigenic)

A

EPEC Infantile diarrhea

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19
Q

EPEC
Virulence factor: ____

A

Pili - pathogenicity of islands

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20
Q

Lesions called “attaching and effacing lesions

A

Enteropathogenic E. coli or EPEC

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21
Q

produce dysentery-like diarrhea (watery to bloody diarrhea with RBC, mucus and neutrophils)

A

EIEC Shigella-like disease

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22
Q

EIEC
Virulence factor:
Test:

A

Invasin
Sereny test

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23
Q

EAEC 2 types

A
  1. Diffusely adherent E. coli (DAEC)
  2. Enteroaggregative E. coli (EAEC)
24
Q

Type of EAEC that causes UTI (chronic/recurring) and diarrhea

A

Diffusely adherent E. coli (DAEC)

25
Q

Type of EAEC that causes “stacked-brick pattern” (no blood but persistent diarrhea) located in cells or between the cells by fimbriae or pili

A

Enteroaggregative E. coli (EAEC)

26
Q

EAEC
Virulence Factor:

A

Aggregative regulator gene (AggR gene)

27
Q

Traveller’s diarrhea
Causes Hemorrhagic colitis
Produces Watery diarrhea

A

Enterotoxigenic or ETEC

28
Q
  • It has a perfused watery stool
  • most common diarrhea
  • self-limiting, non invasive, and no blood and WBC
A

ETEC Montezuma’s revenge

29
Q

presence of colonization factor antigen which produces heat labile and heat stable enterotoxins

A

ETEC Adhesion

30
Q

Grossly bloody diarrhea
Shigella-like toxin

A

Enterohemorrhagic or EHEC

31
Q

EHEC causative agent

A

E.coli 0157:H7

32
Q
  • this produces shigella-like toxins- which is life-threatening (verotoxin), associated with undercooked infected hamburger
  • Colorless
  • (-) in MUG test
A

EHEC

33
Q

EHEC Toxins (2)

A
  1. Verotoxins 1 (VT1)
  2. Verotoxins 2 (VT2)
34
Q

EHEC Toxin that is identical to shigella dysenteriae Type 1 (Shiga Toxin)

A

Verotoxins 1 (VT1)

35
Q

EHEC Toxin that is similar biologically but immunologically different to VT1

A

Verotoxins 2 (VT2)

36
Q
  • most severe manifestation of EHEC
  • undercooked hamburger, dairy products and apple cider
A

EHEC HUS - hemolytic Uremic Syndrome

37
Q

In EHEC it replaces lactose to sorbitol

A

SMAC - Sorbitol Macconkey Agar

38
Q

Extraintestinal E.coli strains (UM)

A
  1. Uropathogenic E. coli (UPEC)
  2. Meningitis/sepsis-associated E. coli (MNEC)
39
Q

Extraintestinal E.coli strains that cause cystitis and acute pyelonephritis

A

Uropathogenic E. coli (UPEC)

40
Q

Extraintestinal E.coli strains that cause neonatal meningitis

A

Meningitis/sepsis-associated E. coli (MNEC)

41
Q
  • 4-methylumbelliferyl-beta-D-glucuronide E. coli broth;
  • (+) result is Electric Blue fluorescence
A

MUG Test

42
Q

Klebsiella spp are:

A
  • Lactose Fermenters
  • Encapsulated
  • Non motile
43
Q

Klebsiella Mucoid:
- ___ is pink and dry
- ___ and ____ are both pink but not darker as E. coli

A

E. coli
Klebsiella and Enterobacter

44
Q

Klebsiella
Virulence factor:
Most resistant antibiotic:

A

Capsule
Ampicillin

45
Q

Ventilator-associated pneumonia, wound infections, UTIs

A

Klebsiella

46
Q
  • Common Name: Fried landers bacillus
  • 2nd cause of UTI
  • Produce secondary pneumonia
A

K. pneumoniae

47
Q
  • classic pneumonia
  • emit bloody sputum
  • associated with community acquired pneumonia that produces a currant jelly like sputum
A

K. pneumoniae

48
Q

K. pneumoniae distinct feature

A

large polysaccharide capsule

49
Q
  • ESBL- Extended-Spectrum Beta-lactamases
  • (+) in Neufeld Quellung
A

K. pneumoniae

50
Q

T/F
In K. pneumoniae, Neufeld Quellung test is used if a bacteria has nucleus

A

FALSE
Neufeld Quellung test is used if a bacteria has CAPSULE

51
Q
  • a disease in nasal mucosa, biochemically inactive
  • Atrophic rhinitis
  • Plasmid-mediated ESBLs
A

K. pneumoniae subsp. ozaenae

52
Q
  • Donovanosis - STD
  • Safety pin appearance
  • Requires additional nutrients for growth
A

K. granulomatis

53
Q

K. granulomatis diagnositc feature

A

DONOVAN body/BEEFY RED ULCER (blue stain with prominent granules); intracellular microorganisms

54
Q
  • Disease similar to K. pneumoniae
  • indole (+)
A

K. oxytoca

55
Q
  • Raoultella planticola
  • which is an environmental microorganism
A

K. planticola

BACTE finals (5 decks)

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