NG 1 Flashcards

1
Q

Defeminizing the male brain

A

There is a compound protein that wont let the estradiol come into the brain so there is a surge center in the female
• In female : makes the surge center
• In male ; testosterone crosses the BBB and then the surge center does NOT develop

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2
Q

Factors affecting age of puberty

A

Breed
Birth season :heifers in fall —>puberty earlier ; ewe in spring —> puberty early in fall different
Density of housing in pigs affect puberty age
Body weight and good nutrition
Biostimulation= intact male to get the women into estrus
The sires Scrotal circumference - female cows

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3
Q

Oogenesis and the arresting in stages

A

In oogenesis, diploid oogonium go through mitosis until one develops into a primary oocyte, which will begin the first meiotic division, but then arrest at meiosis 1 prophase 1; it will finish this division as it develops in the follicle, giving rise to a haploid secondary oocyte and a smaller polar body.
The secondary oocyte begins the second meiosis division and then arrests again at metaphase 2; it will not finish this division unless it is fertilized by a sperm; if this occurs, a mature ovum and another polar body is produced.

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4
Q

Mesonephric duct vs paranephric duct

A

Mesonephric duct is in males and it regresses in females
In males there are serotonin cells [ that allow for mullerian inhibiting factor] and leydig cells [ that have testosterone that stimulates mesonephric ducts and dihydroestrosterone for external genitalia]

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5
Q

The remnant of a miso nephric duct in a female

A

Gartner duct can pharmacist and get inflamed on the floor of the vagina

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6
Q

What makes the uterine horn in a female

A

Para Mesonephric duct

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7
Q

3 parts of the broad ligament

A

Mesovarium … suspends the ovary- Suspensory ligament and proper ligament of the ovary
Mesosalpinx … suspends the uterine tube (salpinx) and cover the lateral side of the ovary.
Mesometrium… suspends the uterine horns and body (round ligament aka the caudal gonad ligament in male )

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8
Q

Parts of uterine tube

A

Infundibulum
Ampulla
Isthmus
Uterotubal junction= end of oviduct

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9
Q

Where anatomic does gestation happen?

A

Uterine horns this pulls the ovary closer to the floor of ventral abdomen with pregnancy

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10
Q

Why are germ cells not destroyed by the immune system?

A

From diploid to aploid cell in the testes - the immune system should not destroy the aploid sperm cell —> this has a blood test barrier that protect the spermatozoa =immunologically privileged site

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11
Q

What part of the sperm has - “ hydrolytic enzymes to penetrate egg

A

Acrosome

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12
Q

What happens to the cranial gonadal ligament in the female versus the male?

A

In the female, it becomes the suspensory ligament of the ovary, and in the male mail it regresses

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13
Q

Explain the CCP, CSP, CSG

A

The Corpus spongiosum penis [CSP] is at the bulb of the penis around the penile urethra- this is part of the root and bulb of the penis with the bulbospongiosus
CSP continues through the

Corpus Cavernosum Penis [CCP] is with the tunica albuginea that has the 2 crus - part of the root allows for erection
— gone in the glans penis bc of the os penis

Corpus Spongiosum glandis is around the os penis and the urethra in the distal part of the penis

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14
Q

Why does the perineal urethrostomy bleed so much?

A

Because of the cavernous tissue that is a part of the bulbospongiosus muscle
THE CSP

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15
Q

What is the muscle that covers the crus of the penis and allows for sustained erection

A

THE ISCHIOCAVERNOUSUS MUSCLE
Has the CCP and the tunica albuginea that helps within it
Also helps with winking in horses

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16
Q

Extrinsic muscles of the penis

A

Retractor penis
Bulbospongiosus
Ischiourethralis
Ischiocavernosum - on crus

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17
Q

Where does the Metanephros migrate in the end of fetal development?

A

The metanephros is the kidney that is made in the pelvis and ascends cranially

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18
Q

Sympathetic innovation of the pelvis

A

Sympathetic innovation is given by the lumbar length nerve that are the Racho, lumbar and origin
Hypo gastric nerves are the only source of sympathetic fibers to the pelvic plexus and the pelvic organs

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19
Q

Where do parasympathetic innovation of the pelvis come from?

A

Pelvic Nerve is made up of sacral nerves one through three
These provide the only parasympathetic innovation to the pelvic plexus

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20
Q

Soul control of the external anal sphincter is given by which nerve

A

Coddle rectal nerve, so cutting it in a perineal hernia correction or anal gland. Resection can cause fecal incontinence.

This comes from the sacral spinal nerve

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21
Q

what lymph node drains all of the pelvic body wall, visceral organs and limbs

A

The medial iliac lymph node, which is a part of the iliosacral, lymph center. It forms a trunk at the aortic hiatus near the diaphragm, where there is a dilation due to all of the lymph moving into it.

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22
Q

Name two muscles that are part of the pelvic diaphragm

A

levator ani helps depress the tail and axes a sling
Coccygeoyus. It is lateral and more cranial than the levator ani

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23
Q

Which nerve helps with the perineal reflex

A

The pudendal nerve that is made up of the sacral and coccygeal nerve areas
These are the spinal nerves

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24
Q

Difference between perineal hernia versus inguinal hernia

A

Interchangeable per elnady- ring is open

A perineal hernia is because the muscles of the perineum, such as the retractor penis and other perineal muscles have separated, allowing for the pelvic contents to touch perineal skin

Inguinal hernia is when the vaginal ring is too wide, allowing for abdominal contents to escape

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25
Q

What’s traveling in this vaginal process a.k.a. vaginal tunic

A

In males, there is this spermatic cord, which contains the genetic femoral nerve, testicular artery vein and vast difference with the cremaster muscle running alongside it (the cremaster muscle is not voluntary action)

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26
Q

The lack of the Muellerian Inhibiting factor from the Sertoli cells results in

A

The female genital tract being formed from the. paramesonephric duct
The lack of Leydig cells in females —> low testosterone —> degeneration of the Mesonephric duct

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27
Q

Removal of the gonads, a.k.a. gonad ectomy results in

A

Very high levels of Grobadotropin, such as FSH and LH
This is because of the lack of negative feedback going to the hypothalamus
So GnRH continues being secreted to the anterior pituitary

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28
Q

What are the two phases of the estrous cycle?

A

The follicular phase, where it is the follicle growing up until ovulation- tight uterus for sperm transport

The luteal phase where progesterone is the main hormone and maintenance of pregnancy, and the corpora luteum is the main structure : from ovulation to corpora Lutea regression
-soft for pregnancy

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29
Q

Is diestrous longer in poly estrous or mono estrous species?

A

Diestrous is longer in monoestrous species Like the dog
They have one to three cycles per year and long periods of Anestrous

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30
Q

What hormone can follicles not become dominant in the prescience of?

A

Progesterone : this is why there has to be an increase in estrogen to cause the inc in FSH and LH to cause ovulation

This is why there is atresia of follicles in the Diestrus and Metestrus phase

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31
Q

differentiate among acrosome reaction, cortical reaction, zona reaction, and slow vs. fast blocks to polyspermy

A

Acrosomal reaction: hydrolytic enzymes open and enter the zona pellucida —> sperm in perivitelline space —> (1) cortical granules from the space go to the zona pellucida to close the oocyte cortical reaction/exocytosis (2) DNA released from sperm (1N) mix Secondary oocyte that is arrested in meiosis 2 metaphase 2 (1N) —>mitosis (PMAT) —> morula [ compaction] —> blastocyst ruptures zona pellucida

Slow block vs fast block by the cortical granules/reaction: Slow block cause polyspermy!!

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32
Q

Where does the sperm get deposited in the pigs and horses

A

In the cervix

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33
Q

two primary causes of poor reproductive performance and low reproductive efficiency in dairy cattle

A

Failure to detect heat and errors in heat detection
Low efficiency y or accuracy

34
Q

Is the rapid or slow phase the more challenging for the sperm

A

It is the rapid phase where they move countercurrent to the uterous the folds of the cervix help with slow phase

Cervix —> uterus —> utero-tubular junction [ they get into the folds and dock here for 4-5 days waiting for the oocyte to come]= site of fertilization

35
Q

sperm capacitation

A

There is increased motility and removed surface proteins from the sperm cell- allow for AR

36
Q

Acrosomal reaction

A

timing and location: bind the zona pellucida (outside of the oocyte)

process: sperm capacitation involves acrosomal reaciton and there is influx of the calcium and there is the fusion of the hydrolytic enzymes to move through the oocyte

consequences - The AR shortens life span and removes the surface proteins —> moves through the acrosin digested path and causes hyperactivated motility through path

37
Q

As we domesticate species we __variability
As we go from domestication—> landrace —> breed—> industrial breed there is ____genetic variability

A

As we domesticate species we increase variability
As we go from domestication—> landrace —> breed—> industrial breed there is decrease genetic variability

38
Q

OVARIAN NEOPLASIA
Smooth surface vs papillary surface

A

Smooth surface= benign
Papillary = metastized

39
Q

Most common and degenerative change to uterus

A

Cystic endometrial hyperplasia
Due to high levels of progesterone that reduce the immune system

Progesterone —> hyperplasia —> fluid —>pyometria
• immune system is downregulated
• Here this leads to pyometria because of bacteremia in the fluid build up

Common in bitch because of prolonged P4 inc in estrus +diestrus

40
Q

Define Freemartin

A

Intersex is common in pig -FREEMARTIN : a male twin and female twin
• fusion of the placenta:
1. Exchange of cells
2. Exchange of hormones
Suppression of female phenotype and enhance the male phenotype : fusion is too late to mess up the gonad but the hypothalamus is messed up
• there are seminal vesicles
• Short vagina
Happens in cattle but all species can have this happen
• usually in large animals that have one offspring cattle ; sometime horses and dogs

41
Q

Brood mare vs performance mare: germinal inclusion cyst

A

Horses have ovulation fossa within and they can drag in the fimbriae epithelium which is more secretory —> cysts in ovulation fossa in aged mares which can affect and block ovulation!!
• not a problem for brood mare because in heat 1x a year; a performance mare will have this risk bc multiple heats

42
Q

Uterine tube: Spherical vs Tubular cyst

A

Spherical cysts are embryologic and dont impact fertility :)

Tubular cyst :( they impact fertility and are post-inflammatory —> block fluid

43
Q

Most common malformation of uterus

A

Segmental aphasia
Unilateral: can reproduce but since P4 only lutenize one side —> multiple cycles

44
Q

Dog just gave birth and there are cysts on the broad ligament by the uterus- what is it?

A

After parturition then there are longitudinal grooves in the uterus —> the perineum will pinch off and look like cysts but they are NORMAL part of post-partum events

45
Q

What to do for a BSE in equine

A

Physical exam -conformation, BSC
Reproductive tract- Utrasound, Rectal palpation, Speculum
Diagnostic test: Cytology, Culture (C+C together) and Biopsy

46
Q

You are choosing between 2 mares:
Vulva just above ischium
Vulva below ishial tuber

When would we have to do surgery

A

Choose horse with highest ischium
the vulva should be 80% below the ischium
Prevent the shelving with age
— helps prevent endometiris [prequel to pyometra) and placentitis
—dont wanna do the Caslicks sx with a lot of vulva on the ischium/shelf

47
Q

Determine if a mare is in estrus or diestrus [behavior and uterus/cervical tone]

A

Estrus: estrogen
— soft tone [3] and uterine edema
—cervix flop on the floor of the vagina
— winking and squating

Diestrus : progesterone CL
— Firm tone for cervix and uterus
— Portiovaginallis uteri is firm on speculum - in the middle
— stay away from stallion or kick

Pregnant :
—very firm cervix - hold that shit in

48
Q

Equine insemination options and know the required timing for each
Where to inseminate

A

Live cover - live or assisted
Extended and Fresh/cooled [AI] : 2-3 days - so order when the mare in estrus [after the luteolyse- remove CL]
Frozen [AI]: 6-12 hrs after thawing

Into the cervix mainly (maybe uterine horn)

49
Q

Histotrophic Nutrition

A
50
Q

Understand causes of surgical hemorrhage and the response

A

Iatrogenic, genetic, acquired due to hemodilution, hypotension, trauma

Response: Direct pressure [finger], Hemostat, ligation, electrocautery, Gel foam

Blot not wipe!

51
Q

3 clamp technique

A

3 clamps
A—cut—B—C
B—C—1st throw
B—C flashed —1st throw

B—C flashed —knot

52
Q

Estimating hemorrhage:
Lap sponge entire surface

Lap sponge dripping

Fully saturated 4x4

Puppy pad fully soaked

A

Lap sponge entire surface 75ml

Lap sponge dripping 100ml

Fully saturated 4x4 5ml

Puppy pad fully soaked 500ml

53
Q

Response to hemorrhage if
20% loss
40% loss
>50% loss

A

90 ml/kg is the total blood volume

20% loss- crystalloid [LRS, normosol]
40% loss [hetastarch]
>50% loss fatal

54
Q

Gestation length of different species

A

Mare 11mo
Cow 9mo
Small ruminant 5 mo
Dog 2.5 mo
Pig 3 mo 3 weeks 3 days

55
Q

MRP for species

A

Embryo the uterus @day 5 after ovulation (cow,mare, small ruminant, cat), @3 days after (pig), @10 days after ovulation (dog)

Cow - bovine interfereon tau prevents PGF @ day 15
Mare - embryo is very motile and uterine contractions in diestrus @ 11-16 days
Ewe- Ovine interferon @ day 12-14
Sow -estradiol prevents PGF @day 11
Dog and cat the MRP is unknown

56
Q

Sperm classification - primary vs secondary
Compensible vs non-sepensible

A

Primary is the major abnormalities- in the head/body/or severe tail defects these are made early on in the testis

Secondary is the minor abnormalities like a distal droplet or detached head or tail kinked. These are minor problems due to the storage in the epididymus or maturation.

The compensible are the sperm that CANNOT SWIM STRAIGHT - so increasing the dose with bent tail sperm can still mean the sperm get to oocyte
tail deformity

Non-compensible are sperm with messed up heads or bodies that CAN SWIM STRAIGHT [normal tail]
Increasing the dose does not mean a viable zygote bc shitty sperm can still get to it

57
Q

What time in estrous does prolactin increase

A

It increases in the 2nd half of diestrus in the bitch and Tom

It helps maintain the pregnancy with progesterone

If it was reduced then it would cause abortion

58
Q

Placenta as an endocrine organ

A

Estrogen - made in the fetal gonads that increase water and sodium absorption by moms kidney —> inc placental blood flow
—enlarged fetal gonad [horse]

Progesterone : increase endometrial growth and ; decrease myometrial [uterine contraction decreased]

Placental relaxin [carnivore and horse]
-relaxation pubic symphysis and angiogenesis and glucose delivery to fetus
Co, Sow ovary-relaxing

59
Q

Histotrophic nutrition vs hemotrophic nutrition

A

Histotrophic nutrition : Diffusion of nutrients to the embryo [ chorionic placenta to yolk sac placenta] @ areolae
—Chorionic attaches embryo to uterus - trophoblast and mesoderm

Hemotrophic nutrition : Blood exchange at the chorioallantoic placenta

60
Q

Describe ideal body condition score (BCS) for the pregnant and lactating bitch/queen

ideal body condition score (BCS) for the pregnant and lactating mare/cow

A

Bitch, Queen
5-6/9 is ideal for the breeding of queen or bitch
Athletic bitch - 4/9

Mare
5-6/9 for reproducing mare
-dont want to be underweight will impact foal growth ; overweight will have longer estrous and hyperinsulinemia and increase inflammation

Cow
DAIRY 3-3.5/5
<3 low milk yield
>3.5 lower dry matter intake
>1 BCS loss —> weight loss in lactation will worsen next pregancy
Adjust BCS in late lactation

BEEF 5-6/9
<5 less pregnancy

61
Q

Demonstrate how to determine energy and nutrient requirements for the pregnant and lactating bitch/queen

A

> 120ml/kg BW water [twice the amount for a normal animal]

Bitch Body weight will increase by 15-25% and so the nutritional needs increase by 35-50% through gestation [** If bitch on adult maintenance then by 4 weeks of gestation then switch to gestation lactation lactation diet** ]

Queen on gestation/lactation diet at breeding: need reserves from early gestation for late lactation [ not eating as much]

Monitor BCS of bitch/queen with RER and DER

62
Q

Describe key nutritional factors for the gestating/lactating bitch/queen

A

DHA - brain and neuro development and retinal development -marine fish oil
EPA

High carbohydrate diet [non-fiber], calcium [to avoid hypocalcimea with small breed and big litter], protein

Micronutrients - folic acid, zinc, iron, b vitamin, vitamin A for eyesight

63
Q

Describe how to use an AAFCO statement to determine if a commercial food is appropriate for the pregnant or lactating bitch/queen

A

It will say it is for all life stages or for the gestation and lactation diet

64
Q

Demonstrate how to determine energy and nutrient requirements for the pregnant and lactating mare/cow

A

Least accurate to most: Gross E, Digestable E, Metabolizable energy, Net Energy

Water and energy requirements go up
2x the maintenance [120 ml /kg BW minimum]

Use BCS as a calorie meter

Requirements change throughout- high energy requirement in early lactation and late gestation

Protein: crude protein, metabolizable protein and net protein [dam] —> weight gain and birth weights and immunity
Dietary fat : limited fats : inc PUFAs and Vitamin E together
Calcium: Cow- avoid hypocalcemia [sign =tetany]
Copper: Endochondral ossification - need fetus to have copper stores to avoid physitis
Fiber carbohydrates : GI motility and the rumen mucosa
Non-fiber carbohydrate: horse Euglycemia support
Volatile fatty acid production via carbohydrates for energy

65
Q

Cow/mare ration

A

Forage +/- concentrate
- forage may be able to be sufficient for pregnancy or lactation [may not need concentrate based on BCS]
-NDF: cell wall - higher the NDF the lower the quality of forage
Vitamin and mineral

Cow: Total mixed ration for dairy cow
-Dairy: early gestation there is more bulky food than in late gestation [ NDF guidelines in later gestation is lower]

DM intake - need the fiber in the forage 2%

Go off concentrate 2 weeks prior to weaning - consider rebreeding status and BCS

AVOID tall fescue grass in late gestation = abortion

66
Q

Descent of the testis
Hint: 3 phases

A

Regression of the cranial gonadal ligament [would be suspensory in carnivore female]
Trans abdominal descent
Trans inguinal descent - testosterone and gubernaculum

67
Q

Ectopic ureter
Male vs Female

A

The termination of the ureter outside the trigone of bladder
Male: proximal to urethralis m which is at the pelvic urethra = no incontinence
Female : distal to the urinary sphincter/ vagina/ caudal urethra = incontinence

68
Q

Monitor at birth?
Dog, Horse, Pig, Ruminant

A

Carnivore and horse- the allantoic sac rupture — the amniotic sac has to be removed at birth: the allantoic sac cuts off amnion from chorion

Ruminant and pig - no monitoring needed because the allantoic and amniontic sac are removed together at birth both are fused to chorion

69
Q

How to tell diestrus with cytology
And what does this mean for pregnancy

A

Can see neutrophils in early diestrus
and round parabasalar cells in diestrus

Progesterone and prolactin (2nd month of diestrus)

Preganacy 57.5 days from the start of diestrus

70
Q

Progesterone levels for bitch breeding estrous cycles

And how to measure

A

1ng/ml no literal tissue
2 NG/ml = LH peak (pre ovulation)
5ng/ml = ovulation (has to to estrogen and progesterone increasing in estrus to have standing behavior and ovulation

Chemoluminesce

71
Q

Autolyzed aborted dog fetus +/- epididymitis or orchitis

Diagnosis [1 slide test, 2 PCR, if ++ Culture] and treatment

A

Potential brucellosis canis infection- need to test between breeding and every 3-6 months

If clinical signs and +
PCR/Culture

If clinical signs and—
3 monthly tests of slide needed

If - = no tests

If + =AGID or PCR to rule out false positive

72
Q

What is small animal vaginal cytology used for?

A

Infection or pyometra
CANNOT use cytology to tell for breeding ready

Can only tell if dog under influence of estrogen or not!!!!
* tell with superficial epithelial cells

73
Q

Reproductive goals for beef cows

A

Be in the field for 70 days and get 60% of open cows pregnant every heat (3 tries)- should be about 95% pregnant

74
Q

AM-PM rule

A

In ruminants and in pigs

In ruminants if you see signs of stress, then 12 hours later, artificially inseminate into the uterine body

75
Q

Synch protocols

A

• OV-sync [Dairy]- handle 4 times GnRH CIDR for 7 days, PGF, 2 days later GnRH, 12 hrs later AI
• CO-sync [Beef]-handle 3 times GnRH CIDR for 7 days, PGF, 2-3 days hrs later GnRH+AI
• Select-Sync – GnRH and CIDR then 7 days later PGF – check for signs of heat at 6 days and AI
• Pre-Sync- NO CIDR : this is just 2 rounds of PGF 14 days apart

76
Q

Red bag abortion

A
77
Q

Which food animal cannot use CIDR?

A

Pigs -have to use synthetic progesteogen
The CL forms at day 12 so saves 3 days of diestrus (15 days) in pigs

78
Q

Estrus detection and illication

A

Small ruminant and pigs show good signs of estrus if housed away from male for long then exposed at time of puberty —> induce puberty then estrus

79
Q

CL dependent vs CL independent preganacy species

A

CL dependent : Sow, Goat/Doe, Bitch, Queen
CL independent where placenta takes over progesterone 1/2 way through: Horse Cow Ewe

80
Q

Proper ligament of the ovary connects to

A

Uterine tube - mare and carnivore
Broad ligament -ruminant and pig

81
Q

Uterine artery from the

A

External iliac in mare

Internal iliac in cow and sow