NG 1 Flashcards

1
Q

Defeminizing the male brain

A

There is a compound protein that wont let the estradiol come into the brain so there is a surge center in the female
• In female : makes the surge center
• In male ; testosterone crosses the BBB and then the surge center does NOT develop

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2
Q

Factors affecting age of puberty

A

Breed
Birth season :heifers in fall —>puberty earlier ; ewe in spring —> puberty early in fall different
Density of housing in pigs affect puberty age
Body weight and good nutrition
Biostimulation= intact male to get the women into estrus
The sires Scrotal circumference - female cows

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3
Q

Oogenesis and the arresting in stages

A

In oogenesis, diploid oogonium go through mitosis until one develops into a primary oocyte, which will begin the first meiotic division, but then arrest at meiosis 1 prophase 1; it will finish this division as it develops in the follicle, giving rise to a haploid secondary oocyte and a smaller polar body.
The secondary oocyte begins the second meiosis division and then arrests again at metaphase 2; it will not finish this division unless it is fertilized by a sperm; if this occurs, a mature ovum and another polar body is produced.

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4
Q

Mesonephric duct vs paranephric duct

A

Mesonephric duct is in males and it regresses in females
In males there are serotonin cells [ that allow for mullerian inhibiting factor] and leydig cells [ that have testosterone that stimulates mesonephric ducts and dihydroestrosterone for external genitalia]

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5
Q

The remnant of a miso nephric duct in a female

A

Gartner duct can pharmacist and get inflamed on the floor of the vagina

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6
Q

What makes the uterine horn in a female

A

Para Mesonephric duct

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7
Q

3 parts of the broad ligament

A

Mesovarium … suspends the ovary- Suspensory ligament and proper ligament of the ovary
Mesosalpinx … suspends the uterine tube (salpinx) and cover the lateral side of the ovary.
Mesometrium… suspends the uterine horns and body (round ligament aka the caudal gonad ligament in male )

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8
Q

Parts of uterine tube

A

Infundibulum
Ampulla
Isthmus
Uterotubal junction= end of oviduct

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9
Q

Where anatomic does gestation happen?

A

Uterine horns this pulls the ovary closer to the floor of ventral abdomen with pregnancy

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10
Q

Why are germ cells not destroyed by the immune system?

A

From diploid to aploid cell in the testes - the immune system should not destroy the aploid sperm cell —> this has a blood test barrier that protect the spermatozoa =immunologically privileged site

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11
Q

What part of the sperm has - “ hydrolytic enzymes to penetrate egg

A

Acrosome

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12
Q

What happens to the cranial gonadal ligament in the female versus the male?

A

In the female, it becomes the suspensory ligament of the ovary, and in the male mail it regresses

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13
Q

Explain the CCP, CSP, CSG

A

The Corpus spongiosum penis [CSP] is at the bulb of the penis around the penile urethra- this is part of the root and bulb of the penis with the bulbospongiosus
CSP continues through the

Corpus Cavernosum Penis [CCP] is with the tunica albuginea that has the 2 crus - part of the root allows for erection
— gone in the glans penis bc of the os penis

Corpus Spongiosum glandis is around the os penis and the urethra in the distal part of the penis

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14
Q

Why does the perineal urethrostomy bleed so much?

A

Because of the cavernous tissue that is a part of the bulbospongiosus muscle
THE CSP

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15
Q

What is the muscle that covers the crus of the penis and allows for sustained erection

A

THE ISCHIOCAVERNOUSUS MUSCLE
Has the CCP and the tunica albuginea that helps within it
Also helps with winking in horses

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16
Q

Extrinsic muscles of the penis

A

Retractor penis
Bulbospongiosus
Ischiourethralis
Ischiocavernosum - on crus

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17
Q

Where does the Metanephros migrate in the end of fetal development?

A

The metanephros is the kidney that is made in the pelvis and ascends cranially

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18
Q

Sympathetic innovation of the pelvis

A

Sympathetic innovation is given by the lumbar length nerve that are the Racho, lumbar and origin
Hypo gastric nerves are the only source of sympathetic fibers to the pelvic plexus and the pelvic organs

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19
Q

Where do parasympathetic innovation of the pelvis come from?

A

Pelvic Nerve is made up of sacral nerves one through three
These provide the only parasympathetic innovation to the pelvic plexus

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20
Q

Soul control of the external anal sphincter is given by which nerve

A

Coddle rectal nerve, so cutting it in a perineal hernia correction or anal gland. Resection can cause fecal incontinence.

This comes from the sacral spinal nerve

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21
Q

what lymph node drains all of the pelvic body wall, visceral organs and limbs

A

The medial iliac lymph node, which is a part of the iliosacral, lymph center. It forms a trunk at the aortic hiatus near the diaphragm, where there is a dilation due to all of the lymph moving into it.

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22
Q

Name two muscles that are part of the pelvic diaphragm

A

levator ani helps depress the tail and axes a sling
Coccygeoyus. It is lateral and more cranial than the levator ani

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23
Q

Which nerve helps with the perineal reflex

A

The pudendal nerve that is made up of the sacral and coccygeal nerve areas
These are the spinal nerves

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24
Q

Difference between perineal hernia versus inguinal hernia

A

Interchangeable per elnady- ring is open

A perineal hernia is because the muscles of the perineum, such as the retractor penis and other perineal muscles have separated, allowing for the pelvic contents to touch perineal skin

Inguinal hernia is when the vaginal ring is too wide, allowing for abdominal contents to escape

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25
What’s traveling in this vaginal process a.k.a. vaginal tunic
In males, there is this spermatic cord, which contains the genetic femoral nerve, testicular artery vein and vast difference with the cremaster muscle running alongside it (the cremaster muscle is not voluntary action)
26
The lack of the Muellerian Inhibiting factor from the Sertoli cells results in
The female genital tract being formed from the. **paramesonephric duct** The lack of Leydig cells in females —> low testosterone —> degeneration of the Mesonephric duct
27
Removal of the gonads, a.k.a. gonad ectomy results in
Very high levels of Grobadotropin, such as FSH and LH This is because of the lack of negative feedback going to the hypothalamus So GnRH continues being secreted to the anterior pituitary
28
What are the two phases of the estrous cycle?
The follicular phase, where it is the follicle growing up until ovulation- tight uterus for sperm transport The luteal phase where progesterone is the main hormone and maintenance of pregnancy, and the corpora luteum is the main structure : from ovulation to corpora Lutea regression -soft for pregnancy
29
Is diestrous longer in poly estrous or mono estrous species?
Diestrous is longer in monoestrous species Like the dog They have one to three cycles per year and long periods of Anestrous
30
What hormone can follicles not become dominant in the prescience of?
Progesterone : this is why there has to be an increase in estrogen to cause the inc in FSH and LH to cause ovulation This is why there is atresia of follicles in the Diestrus and Metestrus phase
31
differentiate among acrosome reaction, cortical reaction, zona reaction, and slow vs. fast blocks to polyspermy
**Acrosomal reaction**: hydrolytic enzymes open and enter the zona pellucida —> sperm in perivitelline space —> (1) cortical granules from the space go to the zona pellucida to close the oocyte **cortical reaction/exocytosis** (2) DNA released from sperm (1N) mix Secondary oocyte that is arrested in meiosis 2 metaphase 2 (1N) —>mitosis (PMAT) —> morula [ compaction] —> blastocyst ruptures zona pellucida Slow block vs fast block by the cortical granules/reaction: Slow block cause polyspermy!!
32
Where does the sperm get deposited in the pigs and horses
In the cervix
33
two primary causes of poor reproductive performance and low reproductive efficiency in dairy cattle
Failure to detect heat and errors in heat detection Low efficiency y or accuracy
34
Is the rapid or slow phase the more challenging for the sperm
It is the rapid phase where they move countercurrent to the uterous the folds of the cervix help with slow phase Cervix —> uterus —> utero-tubular junction [ they get into the folds and dock here for 4-5 days waiting for the oocyte to come]= site of fertilization
35
sperm capacitation
There is increased motility and removed surface proteins from the sperm cell- allow for AR
36
Acrosomal reaction
timing and location: bind the zona pellucida (outside of the oocyte) process: sperm capacitation involves **acrosomal reaciton** and there is influx of the calcium and there is the fusion of the hydrolytic enzymes to move through the oocyte consequences - The AR shortens life span and removes the surface proteins —> **moves through the acrosin digested path and causes hyperactivated motility through path**
37
As we domesticate species we __variability As we go from domestication—> landrace —> breed—> industrial breed there is ____genetic variability
As we domesticate species we **increase** variability As we go from domestication—> landrace —> breed—> industrial breed there is **decrease** genetic variability
38
OVARIAN NEOPLASIA Smooth surface vs papillary surface
Smooth surface= benign Papillary = metastized
39
Most common and degenerative change to uterus
Cystic endometrial hyperplasia Due to high levels of progesterone that reduce the immune system Progesterone —> hyperplasia —> fluid —>pyometria • immune system is downregulated • Here this leads to pyometria because of bacteremia in the fluid build up **Common in bitch because of prolonged P4 inc in estrus +diestrus**
40
Define Freemartin
Intersex is common in pig -FREEMARTIN : a male twin and female twin • fusion of the placenta: 1. Exchange of cells 2. Exchange of hormones Suppression of female phenotype and enhance the male phenotype : fusion is too late to mess up the gonad but the hypothalamus is messed up • there are seminal vesicles • Short vagina Happens in cattle but all species can have this happen • usually in large animals that have one offspring cattle ; sometime horses and dogs
41
Brood mare vs performance mare: **germinal inclusion cyst**
Horses have ovulation fossa within and they can drag in the fimbriae epithelium which is more secretory —> **cysts in ovulation fossa in aged mares which can affect and block ovulation!!** • not a problem for brood mare because in heat 1x a year; a performance mare will have this risk bc multiple heats
42
Uterine tube: Spherical vs Tubular cyst
Spherical cysts are embryologic and dont impact fertility :) Tubular cyst :( they impact fertility and are post-inflammatory —> block fluid
43
Most common malformation of uterus
Segmental aphasia Unilateral: can reproduce but since P4 only lutenize one side —> multiple cycles
44
Dog just gave birth and there are cysts on the broad ligament by the uterus- what is it?
After parturition then there are longitudinal grooves in the uterus —> the perineum will pinch off and look like cysts but they are NORMAL part of post-partum events
45
What to do for a BSE in equine
Physical exam -conformation, BSC Reproductive tract- Utrasound, Rectal palpation, Speculum Diagnostic test: Cytology, Culture (C+C together) and Biopsy
46
You are choosing between 2 mares: Vulva just above ischium Vulva below ishial tuber When would we have to do surgery
Choose horse with highest ischium **the vulva should be 80% below the ischium** Prevent the shelving with age — helps prevent endometiris [prequel to pyometra) and placentitis —dont wanna do the **Caslicks sx** with a lot of vulva on the ischium/shelf
47
Determine if a **mare** is in estrus or diestrus [behavior and uterus/cervical tone]
Estrus: estrogen — soft tone [3] and uterine edema —cervix flop on the floor of the vagina — winking and squating Diestrus : progesterone CL — Firm tone for cervix and uterus — Portiovaginallis uteri is firm on speculum - in the middle — stay away from stallion or kick Pregnant : —very firm cervix - hold that shit in
48
Equine insemination options and know the required timing for each Where to inseminate
Live cover - live or assisted Extended and Fresh/cooled [AI] : 2-3 days - so order when the mare in estrus [after the luteolyse- remove CL] Frozen [AI]: 6-12 hrs after thawing Into the cervix mainly (maybe uterine horn)
49
Histotrophic Nutrition
50
Understand causes of surgical hemorrhage and the response
Iatrogenic, genetic, acquired due to hemodilution, hypotension, trauma Response: Direct pressure [finger], Hemostat, ligation, electrocautery, Gel foam Blot not wipe!
51
3 clamp technique
3 clamps A—cut—B—C B—C—1st throw B—C flashed —1st throw B—C flashed —knot
52
Estimating hemorrhage: Lap sponge entire surface Lap sponge dripping Fully saturated 4x4 Puppy pad fully soaked
Lap sponge entire surface 75ml Lap sponge dripping 100ml Fully saturated 4x4 5ml Puppy pad fully soaked 500ml
53
Response to hemorrhage if 20% loss 40% loss >50% loss
*90 ml/kg is the total blood volume* 20% loss- crystalloid [LRS, normosol] 40% loss [hetastarch] >50% loss fatal
54
Gestation length of different species
Mare 11mo Cow 9mo Small ruminant 5 mo Dog 2.5 mo Pig 3 mo 3 weeks 3 days
55
MRP for species
Embryo the uterus @day 5 after ovulation (cow,mare, small ruminant, cat), @3 days after (pig), @10 days after ovulation (dog) Cow - bovine interfereon tau prevents PGF @ day 15 Mare - embryo is very motile and uterine contractions in diestrus @ 11-16 days Ewe- Ovine interferon @ day 12-14 Sow -estradiol prevents PGF @day 11 Dog and cat the MRP is unknown
56
Sperm classification - primary vs secondary Compensible vs non-sepensible
Primary is the major abnormalities- in the head/body/or severe tail defects these are made early on in the testis Secondary is the minor abnormalities like a distal droplet or detached head or tail kinked. These are minor problems due to the storage in the epididymus or maturation. The compensible are the sperm that CANNOT SWIM STRAIGHT - so increasing the dose with bent tail sperm can still mean the sperm get to oocyte — **tail deformity** Non-compensible are sperm with messed up heads or bodies that CAN SWIM STRAIGHT [normal tail] Increasing the dose does not mean a viable zygote bc shitty sperm can still get to it
57
What time in estrous does prolactin increase
It increases in the 2nd half of diestrus in the bitch and Tom It helps maintain the pregnancy with progesterone If it was reduced then it would cause abortion
58
Placenta as an endocrine organ
Estrogen - made in the fetal gonads that increase water and sodium absorption by moms kidney —> inc placental blood flow —enlarged fetal gonad [horse] Progesterone : increase endometrial growth and ; decrease myometrial [uterine contraction decreased] Placental relaxin [carnivore and horse] -relaxation pubic symphysis and angiogenesis and glucose delivery to fetus Co, Sow ovary-relaxing
59
Histotrophic nutrition vs hemotrophic nutrition
Histotrophic nutrition : **Diffusion of nutrients to the embryo [ chorionic placenta to yolk sac placenta] @ areolae** —Chorionic attaches embryo to uterus - trophoblast and mesoderm Hemotrophic nutrition : Blood exchange at the chorioallantoic placenta
60
Describe ideal body condition score (BCS) for the pregnant and lactating bitch/queen ideal body condition score (BCS) for the pregnant and lactating mare/cow
Bitch, Queen 5-6/9 is ideal for the breeding of queen or bitch Athletic bitch - 4/9 Mare 5-6/9 for reproducing mare -dont want to be underweight will impact foal growth ; overweight will have longer estrous and hyperinsulinemia and increase inflammation Cow DAIRY 3-3.5/5 <3 low milk yield >3.5 lower dry matter intake >1 BCS loss —> weight loss in lactation will worsen next pregancy *Adjust BCS in late lactation* BEEF 5-6/9 <5 less pregnancy
61
Demonstrate how to determine energy and nutrient requirements for the pregnant and lactating bitch/queen
>120ml/kg BW water [twice the amount for a normal animal] Bitch Body weight will increase by 15-25% and so the nutritional needs increase by 35-50% through gestation [** If bitch on adult maintenance then by *4 weeks of gestation* then switch to gestation lactation lactation diet** ] Queen on **gestation/lactation diet at breeding**: need reserves from early gestation for late lactation [ not eating as much] Monitor BCS of bitch/queen with RER and DER
62
Describe key nutritional factors for the gestating/lactating bitch/queen
DHA - **brain and neuro development and retinal development** -marine fish oil EPA High carbohydrate diet [non-fiber], calcium [to avoid hypocalcimea with small breed and big litter], protein Micronutrients - folic acid, zinc, iron, b vitamin, vitamin A for eyesight
63
Describe how to use an AAFCO statement to determine if a commercial food is appropriate for the pregnant or lactating bitch/queen
It will say it is for all life stages or for the gestation and lactation diet
64
Demonstrate how to determine energy and nutrient requirements for the pregnant and lactating mare/cow
Least accurate to most: Gross E, Digestable E, Metabolizable energy, Net Energy Water and energy requirements go up 2x the maintenance [120 ml /kg BW minimum] Use BCS as a calorie meter **Requirements change throughout- high energy requirement in early lactation and late gestation** Protein: crude protein, metabolizable protein and net protein [dam] —> weight gain and birth weights and *immunity* Dietary fat : limited fats : inc PUFAs and Vitamin E together Calcium: Cow- avoid hypocalcemia [sign =tetany] Copper: **Endochondral ossification** - need fetus to have copper stores to avoid physitis Fiber carbohydrates : GI motility and the rumen mucosa Non-fiber carbohydrate: horse Euglycemia support Volatile fatty acid production via carbohydrates for energy
65
Cow/mare ration
Forage +/- concentrate - forage may be able to be sufficient for pregnancy or lactation [may not need concentrate based on BCS] -**NDF: cell wall - higher the NDF the lower the quality of forage** Vitamin and mineral Cow: Total mixed ration for dairy cow -Dairy: early gestation there is more bulky food than in late gestation [ NDF guidelines in later gestation is lower] DM intake - need the fiber in the forage 2% Go off concentrate 2 weeks prior to weaning - consider rebreeding status and BCS AVOID *tall fescue grass* in late gestation = abortion
66
Descent of the testis Hint: 3 phases
Regression of the cranial gonadal ligament [would be suspensory in carnivore female] Trans abdominal descent Trans inguinal descent - testosterone and gubernaculum
67
Ectopic ureter Male vs Female
The termination of the ureter outside the trigone of bladder Male: proximal to urethralis m which is at the pelvic urethra = no incontinence Female : distal to the urinary sphincter/ vagina/ caudal urethra = incontinence
68
Monitor at birth? Dog, Horse, Pig, Ruminant
Carnivore and horse- the allantoic sac rupture — the amniotic sac has to be removed at birth: the allantoic sac cuts off amnion from chorion Ruminant and pig - no monitoring needed because the allantoic and amniontic sac are removed together at birth **both are fused to chorion**
69
How to tell diestrus with cytology And what does this mean for pregnancy
Can see neutrophils in early diestrus and round parabasalar cells in diestrus Progesterone and prolactin (2nd month of diestrus) Preganacy 57.5 days from the start of diestrus
70
Progesterone levels for bitch breeding estrous cycles And how to measure
1ng/ml no literal tissue 2 NG/ml = LH peak (pre ovulation) 5ng/ml = ovulation (has to to estrogen and progesterone increasing in estrus to have standing behavior and ovulation *Chemoluminesce*
71
Autolyzed aborted dog fetus +/- epididymitis or orchitis Diagnosis [1 slide test, 2 PCR, if ++ Culture] and treatment
Potential brucellosis canis infection- need to test between breeding and every 3-6 months If clinical signs and + PCR/Culture If clinical signs and— 3 monthly tests of slide needed If - = no tests If + =AGID or PCR to rule out false positive
72
What is small animal vaginal cytology used for?
Infection or pyometra CANNOT use cytology to tell for breeding ready Can only tell if dog under influence of estrogen or not!!!! * tell with superficial epithelial cells
73
Reproductive goals for beef cows
Be in the field for 70 days and get 60% of open cows pregnant every heat (3 tries)- should be about 95% pregnant
74
AM-PM rule
In ruminants and in pigs In ruminants if you see signs of stress, then 12 hours later, artificially inseminate into the uterine body
75
Synch protocols
• OV-sync [Dairy]- handle 4 times GnRH CIDR for 7 days, PGF, 2 days later GnRH, 12 hrs later AI • CO-sync [Beef]-handle 3 times GnRH CIDR for 7 days, PGF, 2-3 days hrs later GnRH+AI • Select-Sync – GnRH and CIDR then 7 days later PGF – check for signs of heat at 6 days and AI • Pre-Sync- NO CIDR : this is just 2 rounds of PGF 14 days apart
76
Red bag abortion
77
Which food animal cannot use CIDR?
Pigs -have to use synthetic progesteogen The CL forms at day 12 so saves 3 days of diestrus (15 days) in pigs
78
Estrus detection and illication
Small ruminant and pigs show good signs of estrus if housed away from male for long then exposed at time of puberty —> induce puberty then estrus
79
CL dependent vs CL independent preganacy species
CL dependent : Sow, Goat/Doe, Bitch, Queen CL independent where placenta takes over progesterone 1/2 way through: Horse Cow Ewe
80
Proper ligament of the ovary connects to
Uterine tube - mare and carnivore Broad ligament -ruminant and pig
81
Uterine artery from the
External iliac in mare Internal iliac in cow and sow