Next Generation Sequencing

Question 1

What is the process by which dsDNA clusters are generated from a single strand of DNA?

Answer 1

Bridge Amplification

Question 2

In what areas of DNA are 85% of disease-causing mutations found?

Answer 2

Exons

Question 3

What type of sequencing would allow for the specific analysis of the transcriptome?

Answer 3

Whole RNA Sequencing

Question 4

What are the major differences in between whole genome- and whole exome sequencing that makes whole exom sequencing more practical?

Answer 4

Whole Exome Sequencing is cheaper and takes up less storage data

Question 5

What essential additional step is required to do Whole Exome Sequencing that is not used in whole genome sequencing?

Answer 5

Biotin-labeled RNA that correspind to exons in the genome are added to enrich for specific fragments

Question 6

What does CRISPR stand for?

Answer 6

Clustered Regularly Interspaced Short Palindromic Repeats

Question 7

What is the biological origin of CRISPR technology?

Answer 7

It arose as part of the bacterial innate immune system

Question 8

What are the requirements of the CRISPR system?

Answer 8

guideRNA expression, Caspase 9 expression; presence of a PAM site, (+/-) Donor DNA

Question 9

For what condition will the first human trials of the CRISPR system be used for?

Answer 9

Leber congenital amaurosis

Question 10

What is the function of PAM sites in the use of CRISPR?

Answer 10

It localizes guider RNA