Molecular Methods II Flashcards
What is the name of the process of base pairing between complementary single-stranded nucleotides from two different sources under highly defined conditions
Hybridization
What is a probe?
A short piece of ssDNA labeled with detectable molecule
What is the use of probes?
Allows identification of the presence or absence of a specific DNA/RNA/microRNA
How can probes be used to diagnose Sickle Cell Anemia?
A probe can be generated that specifically recognizes the mutant version of the beta-globin gene
What type of biomolecule is detected using Southern Blotting?
DNA
What does RFLP stand for? What is it?
Restriction Fragment Length Polymorphism; A genetic variant that alters restriction sites and/or the sizes of restriction fragments
What is a polymorphism?
A harmless and infrequent variation typically occuring in non-coding regions of the genome
What are the two DNA variations associated with RFLP ?
Single base changes (SNP) and Variable number tandem repeats
What are the stages of each PCR cycle in order?
Denaturation, annealing, extension
How can PCR be used to detect HIV?
Primers around an HIV sequence of DNA are used to amplify potential viral DNA in a sample
What unique enzyme is used in RT-PCR?
Reverse Transcriptase
What molecule is measured using RT-PCR?
mRNA
What type of technology can be used to determine the gene expression patterns present in a particular tissue, cell type, or developmental stage?
Microarrays
What physical feature allows mRNA to be specifically isolated from a sample?
Poly-A Tail
