neurogenesis Flashcards
1
Q
what is a TAP cell?
A
transit amplifying cell
rapidly dividing differentiating cell
2
Q
how is it tested if a prospective hypothalamic is multipotent and already able to instrinsically self renew (no extrinsic siganls required?
A
- isolate a chick prosepctive hypothalamic cell
- take a chick embryo at time hyp prog develop - slice embryo and get rid of slices above an below
- test va insitu hybridisation that cells are Fgf10+ (pure) - and there is no contamination e.g. check for foxg1 to see is some telencephalic cells contaiminating
- culture ex vivo in 3D collagen matrix
- test if grow and take samples and check for differentiated signals via immunohistochemistry and in situ hybridisation
3
Q
what signals were observed after 5 days from isolated fgf10+ cell?
A
- islet 1 (ant hyp prog cell)
- six3 (ant + tub hyp prog cell)
- emx2 (post hyp cell)
- shh (expressed all over?)
4
Q
what is foxg1 a marker for?
A
telencephalon
5
Q
what is a label retaining cell?
A
- a slow dividing stem like cell which can self renew or give rise to a TAP progeniter population
e. g. fgf10+ hypothalamic progenitor cell
6
Q
how can you tell is a cell is rapidly dividing?
A
Dil is diluted rapidly
7
Q
what does a hypothalamic progentior cell give rise to?
A
- frst gives rise to anterior progenitor hyp cells
- next gives rise to post/mammillary hyp cells
- some are retained as tuberal hyp cells
8
Q
what is the difference between a pre-hypothalami cell and a hypothalamc progenitor?
A
pre-hypothlamaic cell - shh+
hypothalamic progenitor - fgf10+
