l9 Flashcards
1
Q
how do we prove that tanycytes are a stem cell and not just a progenitor cell?
A
in vitro studeis to show that tanycyes exhibit neural stem cell properties
- dissect hypothalamic region and dissociate population so have single cells
- 1 cell in each dish
- incubate with growth factors
- allow cells to divide
- see if cell can differentiate into different cell types
- identify by different hypothalamic markers
- MULTIPOTENCY
- arcuate eurons differentiate from hypothalamic neurospheres
6) to see f stem cell could dissociate neurophere nack into single cell and see if it can produce neurophere again
2
Q
what is hesS
A
a stem cell promoter
3
Q
what is HesS::Cre-ERT2 mouse being used to see?
A
being used to see if corticosterone stimulates tanycytes
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