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Question 1

what makes a neural cell multipotent?

Answer 1

• if it can self renew

- can give rise to both neurons and glia

Question 2

what does EdU do?

Answer 2

EdU is a thymidine homolog - incorperates into new DNA during synthesis (S-phase)
- it labels newborn cells

Question 3

How can you lneage trace from a-tanycytes?

Answer 3

• transgenic mouse - clone Cre-ERT2 (Cre fused to oestrogen ligand) downstream of a GLAST promoter
• cross with a mouse which has a stop codon downstream of constitutive promoter (rosa26). and a GFP downstream of the STOP codon
• when tamoxifen injected - cre-ERT2 is activated and cuts lox sites in GLAST expressing cells (a tanycytes)
• cutting of lox allows GFP to be expressed
• GFP is only expressed in cells expressing GLAST and their progeny (a-tanycytes and progeny)

Question 4

can a-tanycytes self renew?

Answer 4

yes

• they can give rise to more tanycytic populations and can also give rise to neurons and astrocytes
• they are neurogenic

Question 5

how is neurogenesis under caged conditions in mice?

Answer 5

under normal caged conditions levels of neurogenesis are low

• this because the mice are well looked after and in a rach environment - they are not stressed
• stress promotes neurogenesis