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Histology Winter Semester > Nerve tissue (Lecture 9) > Flashcards

Nerve tissue (Lecture 9) Flashcards

1
Q

What are the neurohistological methods?

A

Staining:

  • Nissl - toluidine blue, thionin - cytology of neurons (Nissl’s bodies, nucleu and nucleoli)
  • Luxol blue - myelin sheets, phospholipids
  • Weigert - myelin sheets (mordant - chromium salts)

Impregnation:

  • Demonstrates neurons and their processes - precipitates of metal silver on neurofibrils (neurotubules and neurofilaments).
  • Demonstrates glial cells.

Immunohistochemistry:

  • Demonstrates specific antigens of neurons (neurotubules) and neuroglia (GFAP - intermediate filaents - nestin).
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2
Q

What is this a scheme of?

A

A neuron

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3
Q

What are the types of neurons?

A

Unipolar - amacrine cells

Bipolar - brain nerve ganglion

Pseudounipolar - spinal ganglion

Multipolar:

a) Stellate - spinal cord
b) Purkynje - cerebellar cortex
c) Pyramidal - brain cortex

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4
Q

What is this slide?

A

Spinal cord

Multipolar neurons (arrows pointing at these)

HE staining

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5
Q

What is this slide?

A

Spinal cord

Multipolar neurons

Nissl stain

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6
Q

What is this slide?

A

Cerebral cortex

Pyrimidal cells (aroow)

Nissl method

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7
Q

What is this slide?

A

Cerebral cortex

Nissl blue

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8
Q

What is this slide?

A

Cerebral cortex

Silver impregnation

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9
Q

What is this slide?

A

Pyramidal cell

Silver impregnation

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10
Q

What is this slide?

A

Cerebellum

Nissl stain

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11
Q

What is this slide?

A

Cerebellum

Silver impregnation

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12
Q

What are neuroglia?

A

CNS:

  • Macroglia - astrocytes fibrillar and plasmatic, Bergmann cells, Muller cells, pituicytes, ependymal cells
  • Oligodendroglia - myelinization
  • Microglia - phagocytosis (mesenchyal origin)

PNS:

  • Schwann cells - insulation and myelinization
  • Satelite cells - insulation of ganglionic neurons
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13
Q

What is this slide?

A

Protoplasmic astrocytes

Silver impregnation

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14
Q

What is this slide?

A

Fibrilar astrocyes

Gold enhanced silver impregnation

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15
Q

What is this slide?

A

Oligodendrocyte

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16
Q

What is this slide?

A

Microglia

Silver impregation

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17
Q

What are nerve fibres?

A
  • Naked - without glial sheet
  • Only rarely found
  • Intraepithelial free nerve endings
  • Cornea of the eye

With glial sheet:

  • In CNS - processes of glial cells
  • In PNS - Schwann cells

Myelinated - internodal segment of the myelin sheet

  • In CNS - in the cytoplasm of the oligodendrocyte processus
  • In PNS - in the cytoplasm of the Schwann cell
18
Q

What is this slide?

A

Non myelinated nerve fibres in the PNS

Schwann’s cells

19
Q

What is the scheme of?

A

Myelinated fibres in the PNS

20
Q

What is this a scheme of?

A

Myelinated fibres in the CNS

21
Q

What is this slide?

A

Spinal cord

HE staining

22
Q

What is this slide?

A

Spinal cord

Weigert’s method

23
Q

What is this slide?

A

Spinal cord

Weigerts method

24
Q

What is this slide?

A

Spinal cord

White matter

Impregnation

25
Q

What is this slide?

A

Myelinated nerve fibre

Cytoplasm of schwann cell

26
Q

What is this slide?

A

Unmyelinated nerve fibres

Post ganglionic sympathetic nerve fibres

Unmyelinated nerve fibres are also enveloped and nurtured by Schwann cell cytoplasm

27
Q

What is this slide?

A

Periferal nerve

Cross section

HE staining

28
Q

What is this slide?

A

Periferal nerve

Cross section

HE staining

29
Q

What is this slide?

A

Periferal nerve

Longitudinal section

Dominici method

Arrows point to axons

30
Q

What is this slide?

A

Periferal nerves

Cross section

31
Q

What is this slide?

A

Periferal nerve

Cross section

32
Q

What is this slide?

A

Periferal nerve

Cross section

33
Q

What is this slide?

A

Periferal nerve

Longitudinal section

Schmidt-Lantermann’s incisures

34
Q

What is this slide?

A

Periferal nerve

Longitudinal section

Node of Ranvier (arrow)

Dominici method

35
Q

What is this slide?

A

Spinal ganglion

HE staining

36
Q

What is this slide?

A

Spinal ganglion

Impregnation method

37
Q

What is this slide?

A

Autonomic ganglion - Sympathetic

HE staining

Multipolar ganglion cells

38
Q

What is this slide?

A

Autonomic ganglion - Sympathetic

Silver impregnation

39
Q

What is this scheme?

A

Motor end plate

40
Q

What is this slide?

A

Motor end plate

41
Q

What is this scheme of?

A

Muscle spindle

42
Q

What is this slide?

A

Muscle spindle

Histology Winter Semester (10 decks)

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