Nerve tissue (Lecture 9) Flashcards
What are the neurohistological methods?
Staining:
- Nissl - toluidine blue, thionin - cytology of neurons (Nissl’s bodies, nucleu and nucleoli)
- Luxol blue - myelin sheets, phospholipids
- Weigert - myelin sheets (mordant - chromium salts)
Impregnation:
- Demonstrates neurons and their processes - precipitates of metal silver on neurofibrils (neurotubules and neurofilaments).
- Demonstrates glial cells.
Immunohistochemistry:
- Demonstrates specific antigens of neurons (neurotubules) and neuroglia (GFAP - intermediate filaents - nestin).
What is this a scheme of?
A neuron
What are the types of neurons?
Unipolar - amacrine cells
Bipolar - brain nerve ganglion
Pseudounipolar - spinal ganglion
Multipolar:
a) Stellate - spinal cord
b) Purkynje - cerebellar cortex
c) Pyramidal - brain cortex
What is this slide?
Spinal cord
Multipolar neurons (arrows pointing at these)
HE staining
What is this slide?
Spinal cord
Multipolar neurons
Nissl stain
What is this slide?
Cerebral cortex
Pyrimidal cells (aroow)
Nissl method
What is this slide?
Cerebral cortex
Nissl blue
What is this slide?
Cerebral cortex
Silver impregnation
What is this slide?
Pyramidal cell
Silver impregnation
What is this slide?
Cerebellum
Nissl stain
What is this slide?
Cerebellum
Silver impregnation
What are neuroglia?
CNS:
- Macroglia - astrocytes fibrillar and plasmatic, Bergmann cells, Muller cells, pituicytes, ependymal cells
- Oligodendroglia - myelinization
- Microglia - phagocytosis (mesenchyal origin)
PNS:
- Schwann cells - insulation and myelinization
- Satelite cells - insulation of ganglionic neurons
What is this slide?
Protoplasmic astrocytes
Silver impregnation
What is this slide?
Fibrilar astrocyes
Gold enhanced silver impregnation
What is this slide?
Oligodendrocyte
What is this slide?
Microglia
Silver impregation
What are nerve fibres?
- Naked - without glial sheet
- Only rarely found
- Intraepithelial free nerve endings
- Cornea of the eye
With glial sheet:
- In CNS - processes of glial cells
- In PNS - Schwann cells
Myelinated - internodal segment of the myelin sheet
- In CNS - in the cytoplasm of the oligodendrocyte processus
- In PNS - in the cytoplasm of the Schwann cell
What is this slide?
Non myelinated nerve fibres in the PNS
Schwann’s cells
What is the scheme of?
Myelinated fibres in the PNS
What is this a scheme of?
Myelinated fibres in the CNS
What is this slide?
Spinal cord
HE staining
What is this slide?
Spinal cord
Weigert’s method
What is this slide?
Spinal cord
Weigerts method
What is this slide?
Spinal cord
White matter
Impregnation
What is this slide?
Myelinated nerve fibre
Cytoplasm of schwann cell
What is this slide?
Unmyelinated nerve fibres
Post ganglionic sympathetic nerve fibres
Unmyelinated nerve fibres are also enveloped and nurtured by Schwann cell cytoplasm
What is this slide?
Periferal nerve
Cross section
HE staining
What is this slide?
Periferal nerve
Cross section
HE staining
What is this slide?
Periferal nerve
Longitudinal section
Dominici method
Arrows point to axons
What is this slide?
Periferal nerves
Cross section
What is this slide?
Periferal nerve
Cross section
What is this slide?
Periferal nerve
Cross section
What is this slide?
Periferal nerve
Longitudinal section
Schmidt-Lantermann’s incisures
What is this slide?
Periferal nerve
Longitudinal section
Node of Ranvier (arrow)
Dominici method
What is this slide?
Spinal ganglion
HE staining
What is this slide?
Spinal ganglion
Impregnation method
What is this slide?
Autonomic ganglion - Sympathetic
HE staining
Multipolar ganglion cells
What is this slide?
Autonomic ganglion - Sympathetic
Silver impregnation
What is this scheme?
Motor end plate
What is this slide?
Motor end plate
What is this scheme of?
Muscle spindle
What is this slide?
Muscle spindle
