NCBl

Question 1

Retrieve Genome sequence

Answer 1

Go to the gene database of the gene of interest, click genbank view below the genome viewer, go to send to, go to file, go to FASTA, go to create file

Question 2

Retrieve mRNA sequence

Answer 2

Search the gene database of the gene of interest, click reqseq transcripts, find the specific sequence, then click FASTA

Question 3

Retrieve Refseq protein

Answer 3

Search the gene database of the gene of interest, click reqseq proteins, find the specific AA sequence, then click FASTA

Question 4

Find CDS

Answer 4

CDS is on the main page of the database of the gene of interest

Question 5

Video using gene database

Answer 5

Search gene database for a CFTR gene, which brings to a page on CFTR

Genomic context shows where the gene lives
The Annotation Release assembly shows the new updated data on the gene
The location shows the base pair where the gene exists in the chromosome
Diagram show the direction which the gene is read
Conventionally, the chromosome be presented with the shorter arm on top or at the left. The arrows show the direction of the gene which is read.

In the genome browser, green line represents the information on the gene, information on the transcribed mRNA in blue and information on the translated protein in red,

Multiple blue and red lines represent splice variants, variants which can generate different mRNAs.

Experimentally verified transcripts begin with N
Variants beginning with X represent a predicted mRNA transcript or transcribed protein

Zooming on, the larger bars represent exons and the smaller bars represent introns.

An important concept for researchers is clinical variations, when variation in genes have clinical significance. The darker the color of the variant, the more pathogenic or deadly the variation.

SNPs, how individuals differ from one another.
Information on exons and introns all linked to related databases.

Bibliography are selected PubMed citation focusing on the human CFTR gene.
For citations of homologs in other species, click on the final link.

If you need citation focusing on the specific function of a gene, this section on GeneRIFs into function, can be very helpful.

A single sentence taken from the title or abstract that concisely describes the function or functions of a geenin that article.