NCBI Protein Blast and Needle (EMBOSS) from EMBL-EBL

Question 1

NCBL Blast

Answer 1

Press align 2 or more sequences
Copy and paste the protein amino acid sequence into the top window
Do the same for the other protein amino acid sequence below the first one
Check blastp
Then hit blast

Question 2

Interpret the data

Answer 2

If you see a string of letters, that means they are identical , gap means they are penalties
Plus sign means amino acids that are chemically close
Identity: extent of amino acid positions are exact match
Similarity: similarity of conserved matches
How do you know which protein is more similar?

Question 3

Needle (EMBOSS) from EMBL-EBI

Answer 3

Copy and paste both amino sequences in separate boxes and click blast