Cellbiol sequence manipulation site

Question 1

How to use this site

Answer 1

Look at the left side of the page for tools of sequence analysis

Question 2

Generate reverse complimentary sequence tool

Answer 2

Use the tool reverse complementary DNA sequence.

Question 3

What is the difference among reversed sequence, complementary sequence and reverse complementary sequence?

Answer 3

The reverse compliment converts a DNA sequence into its reverse compliment form.
It means it reverse the letters, interchanging them.
A becomes G, G becomes A, C becomes T, T becomes C. It is first reversed and the compliment is found of reverse is found. GACT becomes AGTC.

The complement converts a DNA sequence into cDNA, it swaps the base with its complimentary base.
It means it G becomes C, A becomes T, C becomes G and T becomes A.

Reverse converts a DNA sequence from front to back. It means GAC becomes CAG.

Question 4

ORF open reading frame tool

Answer 4

You also have a few options to specify about the tool:
• Start codon of the ORF can be set to “ATG” or other codons
• Reading frame
• The strand that to be translated. “Direct” refers to forward strand, and “Reverse” for the reverse strand.
• Minimum length of the ORF. This is useful if you have the estimated size of the translated protein.
• Genetic code (standard or mitochondrial) to be used for translating the DNA sequence.

Question 5

What is a open reading frame?

Answer 5

An open reading frame is the part of a reading frame that can be translated. An ORF is a continuous stretch of codons that begins with a start codon and ends at a stop codon. AUG is the start codon and UGA, UAA, and UAG are stop codons.

Question 6

Find restriction enzyme cut sites

Answer 6

Restriction Digest Function - To find whether a particular restriction enzyme can cut the input DNA (up to 3 enzymes) Type in a DNA sequence Restriction Digest to find the whether a particular restriction enzyme cuts the input DNA (up to 3 enzymes)

Restriction Summary- List out the restriction enzymes that cut the input DNA and the location of the cut site (type in a DNA sequence)

Restriction Map - Similar to “restriction summary” but also display the cut site in graphical format

Question 7

Find protein molecular weight and isoelectric point

Answer 7

Use the Protein molecular weight and Protein Isoelectric point function and insert the amino acid sequence