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Mycoviro > MYCOVIRO platings > Flashcards

MYCOVIRO platings Flashcards

1
Q

Most +single-stranded RNA viruses multiply in the cytoplasm
EXCEPT
a. Orthomyxoviridae
b. Picornaviridae
c. Retroviridae
d. Coronaviridae

A

Retroviridae, Orthomyxoviridae

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2
Q

This family consist of viruses which are resistant to the acidity of the gastric juice and are able to infect the intestinal tract
a. Paramyxoviridae
b. Orthomyxoviridae
c. Herpesviridae
d. Adenoviridae

A

Adenoviridae

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3
Q

A 7-month-old boy suddenly developed nausea, vomiting, fever, and abdominal pain. Microbiological examinations showed that the illness was caused by a non-enveloped, ds-RNA virus. This virus
MOST likely belongs to family
a. Picornaviridae
b. Parvoviridae
C. Reoviridae
d. Hepadnaviridae

A

Reoviridae

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4
Q

What do these families of viruses have in common: Herpesviridae, Hepadnaviridae, Poxviridae?
a. -ss-DNA genome
b. +ss-RNA genome
c. icosahedral symmetry
d. envelope

A

envelope

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5
Q

Which of the following will MOST likely cause a disease due to an intense inflammatory reaction?
a. Coronaviridae
b. Picornaviridae
c. Reoviridae
d. Adenoviridae

A

Coronaviridae

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6
Q

Which has ss-RNA, helical and enveloped?
a. Picornaviridae
b. Togaviridae
c. Paramyxoviridae
d. Retroviridae

A

Paramyxoviridae

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7
Q

This is a group of families with negative single stranded RNA genome
a. Lentivirinae
b. Paramyxovirinae
c. Paramyxoviridae
d. Mononegavirales

A

Mononegavirales

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8
Q

Which has helical symmetry, enveloped?
a. Bunyaviridae
b. Togaviridae
c. Picornaviridae
d. Flaviviridae

A

Bunyaviridae

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9
Q

Which is NOT a virus-encoded structure or component
a. glycoprotein
b. envelope
c. Capsid
d. DNA polymerase

A

envelope

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10
Q

Enveloped viruses are more sensitive to adverse environment than do the naked viruses. Which is an enveloped virus family?
a. Picornaviridae
b. Papillomaviridae
c. Poxviridae
d. Adenoviridae

A

Poxviridae

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11
Q

Which is LEAST accurate concerning properties of viruses?
a. Viruses without envelope are usually those with
icosahedral symmetry.
b. Majority of RNA viruses have envelope.
c. Most DNA viruses multiply in the nucleus.
d. Viruses with negative sense RNA usually have icosahedral symmetry

A

Viruses with negative sense RNA usually have
icosahedral symmetry.

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12
Q

Which of the following is true about the smallest animal virus?
a. it has a single-stranded DNA genome
b. It has a single-stranded RNA genome
c. It has a double-stranded RNA genome
d. It has double-stranded DNA genome

A

It has a single-stranded DNA genome

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13
Q

Which family has RNA genome that also serves mRNA?
a. Orthomyxoviridae
b. Adenoviridae
c. Caliciviridae
d. Parvoviridae

A

Caliciviridae

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14
Q

Which has an RNA genome that has a sequence complementary to mRNA?
a. Flaviviridae
b. Paramyxoviridae
c. Togaviridae
d. Reoviridae

A

Paramyxoviridae

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15
Q

Once a virus recognizes and attaches to a specific surface receptor of a cell it will?
a. penetrate the cell
b. start cause the cell to synthesize its parts.
c. bud from the membrane.
d. uncoat

A

penetrate the cell.

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16
Q

Which is true about shell vial culture?
a. The presence of a virus is detected by
immunofluorescence technique
b. The coverslip can still be used for subculture even
after subjecting to immunofluorescence
technique
c. The monolayer of cells is formed in a test tube
d. Only one type of cells is used

A

The presence of a virus is detected by
immunofluorescence technique

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17
Q

This technique allows exponential multiplication of specific target
DNA sequences by a factor of 1 billion
a. Dot blot
b. Real-time PCR
c. Electron microscopy
d. ELISA

A

Real-time PCR

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18
Q

Which is True about primary cell cultures?
a. derived from embryonic cells
b. can be subcultured once or twice
c. contain immortalized cells
d. all of the above

A

can be subcultured once or twice

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19
Q

Clinical specimens for viral studies must be transported at
a. refrigeration temp
b. Room temp
c. -20°C
d. 37°C

A

refrigeration temp

20
Q

Which of the following will NOT support the growth of viruses in culture?
a. chick embryo
b. shell vial culture
c. Animals
d. chocolate agar

A

chocolate agar

21
Q

This technique detects the presence of a virus by use of specific antibody
a. electron microscopy
b. PCR
c. dot blot
d. ELISA

A

ELISA

22
Q

How long does it usually take before the presence of a virus is detected in conventional cell cultures?
a. one week
b. 4 weeks
c. 48 hours
d. 24 hours

A

4 weeks

23
Q

Viruses that cannot be cultured may be diagnosed using
a. Serology
b. PCR
c. Direct Immunofluorescence
d. all of the above

A

Serology
PCR
Direct Immunofluorescence

24
Q

The virus in culture may be identified using the following EXCEPT
a. specific antibody
b. specific primers
c. specific antigen
d. DNA probe

A

specific antigen

25
Q

Which technique is most applicable for examination of specimens suspected of viral infections that produce a characteristic cytopathic effect?
a. PCR
b. Light microscopy
c. Immunofluorescence
d. ELISA

A

Light microscopy

26
Q

True about electron microscopy
a. Very sensitive, can detect a few virus particles in a specimen
b. A virus may be detected but cannot be identified
c. An easy and inexpensive technique to identify a virus from culture
d. The use of virus-specific antibody to a sample facilitates easier detection of virus particles

A

The use of virus-specific antibody to a sample facilitates easier detection of virus particles

27
Q

Example of a continuous cell culture
a. Monkey kidney cell culture
b. Hela cell culture
c. human embryonic kidney cell culture
d. Mouse liver cell culture

A

Hela cell culture

28
Q

Which is NOT TRUE about neutralization test?
a. applicable to viruses that cause CPE
b. can detect antibody to a virus
c. can detect viral antigen
d. can detect hemagglutination activity

A

can detect hemagglutination activity

29
Q

Majority of viruses grow well on
a. cell cultures derived from malignant tissues
b. cell culture derived from embryonic tissues
c. Chick embryo
d. cell culture derived from adult tissues

A

cell culture derived from adult tissues

30
Q

Which of the following is INCORRECT pair?
a. Viral nucleic acid-Southern blotting
b. Viral antigen-Immunofluorescence
c. Virus morphology-electron microscopy
d. Viral RNA-PCR

A

Viral RNA-PCR

31
Q

This is a ds-DNA virus that causes a febrile illness with a rash and is called the sixth childhood diseases.

A

HHV-6

32
Q

Skin lesions of varying stages are seen at one time in
a. Herpes simplex type 1
b. Molluscum contagiosum
c. Chickenpox
d. Smallpox

A

Chickenpox

33
Q

VCA Ag is used to detect antibodies against?
a. EBV
b. Chickenpox virus
c. HSV-1
d. Smallpox virus

A

EBV

34
Q

Which virus particles may be seen under the light microscope?
a. HSV-2
b. Smallpox
c. EBV
d. CMV

A

Smallpox

35
Q

This recurrence of a herpesvirus infection is characteristically painful
a. Shingles
b. EBV infection
c. chickenpox
d. Fever blister

A

Shingles

36
Q

Gingivostomatitis may recur following some stress conditions. The
causative virus is latent in
a. Trigeminal nerve ganglia
b. Sacral nerve ganglia
c. Dorsal root ganglia
d. Posterior root ganglia

A

Trigeminal nerve ganglia

37
Q

Neonatal herpes is most commonly caused by
a. HSV-2
b. EBVV
c. HSV-1
d. CMV

A

HSV-2

38
Q

Which is most likely to be transmitted by blood transfusion?
a. Chickenpox
b. EBV
c. Smallpox virus
d. CMV

A

CMV

39
Q

Which is NOT transmitted through contaminated saliva or respiratory droplets?
a. EBV
b. Cowpox virus
c. VZV
d. HSV-1

A

Cowpox virus

40
Q

Which virus was the first one against which vaccination was first done?
a. CMV
b. Chickenpox
c. Cowpox
d. Variola major

A

Variola major

41
Q

This is a property of Herpesviridae but not of the Poxviridae
a. multiply in the cytoplasm
b. cause vesicular skin lesions
c. mutiply in the nucleus
d. Ds-DNA, enveloped

A

multiply in the cytoplasm

42
Q

Which is INACCURATE about CMV and CMV infections?
a. The infection may be transmitted to the newborn in utero
b. The virus is latent in WBC
c. It is a cause of nasopharyngeal cancer
d. Healthy people usually present asymptomatic or mild
infections

A

It is a cause of nasopharyngeal cancer

43
Q

Which is NOT detected by Tzanck Test?
a. herpes simplex-2
b. herpes simplex-1
c. varicella
d. smallpox

A

smallpox

44
Q

Which distinguishes EBV infection from CMV infection as causes of infectious mononucleosis?
a. Infection of WBC
b. Heterophile antibody test
c. Latency in sacral nerve ganglia
d. Tzanck test

A

Heterophile antibody test

45
Q

A 35-year-old woman suffering from acute lymphocytic leukemia and undergoing chemotherapy suddenly developed small vesicular lesions across her left breast. The lesions were painful and lasted 6
days before clearing. The affected area developed small vesicles which progressed to ulcerated and crusted pustules. She had similar occurrence last year. The appearance of the localized lesions over a specific dermatome (area enervated by sensory nerves) is
characteristic of

A

Zoster

Mycoviro (4 decks)

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