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Mycoviro > mycoplatingssss > Flashcards

mycoplatingssss Flashcards

1
Q

Hypersensitivity and inflammation to cause immunopathogenesis is a property of which of the following viruses?
a. Naked
b. Enveloped
c. Both of the given options
d. Neither of the given options

A

Enveloped

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2
Q

The following are general characteristics of viruses, EXCEPT?
a. Possess their own genomes
b. Grow in cell culture, chick’s embryo and animals
c. Obligate intracellular organisms
d. Can infect unlimited number of hosts

A

d. Can infect unlimited number of hosts

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3
Q

Which of the following viruses have complex structure?
a. Adenovirus
b. Parvovirus
c. Coronavirus
d. Poxvirus

A

Poxvirus

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4
Q

Variolation was first practiced in which of the following countries?
a. China
b. Egypt
c. Turkey
d. Persia

A

China

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5
Q

Which of the following viral components may or may not be present in a virus?
a. viral genome
b. viral capsid
c. Viral envelope
d. None of the given options

A

Viral envelope

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6
Q

Which of the following is/are true of enveloped capsids?
a. Released by cell lysis from host cell
b. Transmitted by fecal oral route
c. Environmentally stable
d. Antibody may be sufficient for immunoprotection

A

Released by cell lysis from host cell

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7
Q

Which of the following events is first in the viral replication cycle?
a. Attachment
b. Penetration
c. Recognition
d. Uncoating

A

Recognition

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8
Q

Which of the following is/are properties of naked capsid?
a. Can dry out and retain infectivity
b. Cell-mediated immunity offers immunoprotection
c. Transmitted by secretions, injections and blood transfusion
d. All of the given options

A

a. Can dry out and retain infectivity

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9
Q

Growth of which of the following viruses will cause lytic changes in the cell culture?
a. Naked
b. Enveloped
c. Both of the given options
d. Neither of the given options

A

Naked and enveloped

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10
Q

Vaccination was officially started by Edward Jenner in May 14, 1796 against which of the following diseases?
a. Influenza
b. Cowpox
c. Smallpox
d. Poliovirus

A

Cowpox

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11
Q

Which of the following viral components determine the structure of the virus?
a. viral genome
b. viral capsid
c. Viral envelope
d. None of the given options

A

viral capsid

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12
Q

Which of the following is NOT part of the nucleocapsid?
a. Genomes
b. Capsomeres
c. Envelope
d. None of the given options

A

Envelope

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13
Q

Which of the following viruses are environmentally stable?
a. Naked
b. Enveloped
c. Both of the given options
d. Neither of the given options

A

Naked

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14
Q

Which of the following is true about the icosahedral viruses?
a. Pentons comprise the 12 vertices
b. Hexons comprise the 20 faces but varies among virus groups
c. Both of the given options
d. Neither of the given options

A

Both of the given options

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15
Q

Among the different changes in the viral replication cycle, which one is used to demonstrate the presence of the
virus is cell culture by doing Immunofluorescence staining?
a. Syncytia formation
b. Lytic changes
c. Formation of early viral protein
d. All of the given options

A

Formation of early viral protein

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16
Q

Which of the following materials cannot be used to collect swabs for viral study?
a. Ca alginate
b. nylon
c. Dacron
d. cotton

A

Ca alginate

17
Q

Which uses enzyme-linked DNA probe?
a. Sandwich ELISA
b. DNA microarray
c. In situ hybridization
d. immunofluorescence

A

Sandwich ELISA

18
Q

If a virus does not produce CPE in cell culture, which would indicates its presence in culture?
a. Formation of syncytia
b. presence of inclusion bodies
c. Hemadsorption
d. ballooning of cells

A

Hemadsorption

19
Q

Clinical specimens for viral study should be transported in
a. Ice or refrigerated
b. frozen form at -70C
c. frozen form at -20C
d. room temperature

A

Ice or refrigerated

20
Q

Which will NOT support the growth of viruses?
a. animal
b. shell vial
c. cell culture
d. blood agar

A

blood agar

21
Q

Which type of cell culture uses a round cover slip where the virus is grown and can be rapidly detected in a few
days?
a. Primary cell culture
b. immunofluorescence
c. shell vial culture
d. in situ hybridization

A

shell vial culture

22
Q

Which is LEAST accurate about qPCR?
a. requires the use of fluorescent dyes
b. detects DNA while being amplified
c. does not require gel electrophoresis
d. requires the use of UV transilluminator

A

requires the use of UV transilluminator

23
Q

A clinical specimen was cultured in a primary cell line. Hemagglutination was positive. The MT identified the
specific virus by reacting it with specific antisera. The following are the reactions that she observed: What is the
identity of the virus?

(?virus + rubella virus antiserum) + rbc → hemagglutination

(?virus + dengue virus antiserum) + rbc → no hemagglutination

(?virus + influenza virus antiserum) + rbc → hemagglutination

(?virus + zika virus antiserum) + rbc → hemagglutination

a. Rubella virus
b. zika virus
c. dengue virus
d. influenza virus

A

dengue virus

24
Q

Which is a direct method to detect viral antigen using enzyme and substrate?
a. immunohistochemistry
b. Neutralization test
c. direct immunofluorescence
d. hemagglutination-inhibition test

A

immunohistochemistry

25
Q

Which can be subcultured for up to 50 times?
a. Primary cell culture
b. shell vial culture
c. Semi-continuous cell culture
d. continuous cell culture

A

Semi-continuous cell culture

26
Q

Which requires conversion of viral RNA to complementary DNA?
a. RT-PCR
b. Conventional PCR
c. FISH
d. Northern blot

A

RT-PCR

27
Q

Which is derived from malignant tissues?
a. Primary cell culture
b. continuous cell culture
c. Semi-continuous cell culture
d. shell vial culture

A

continuous cell culture

28
Q

Which of the following techniques use light microscope?
a. FISH
b. immunofluorescence
c. DNA microarray
d. immunohistochemistry

A

immunohistochemistry

29
Q

Viral genome can be detected directly in tissue by use of
a. Southern blot
b. Dot blot
c. in situ hybridization
d. DNA microarray

A

in situ hybridization

30
Q
A
30
Q
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31
Q
A
32
Q
A

Mycoviro (4 decks)

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