mycoexams Flashcards
Common cold is caused by
a. Mumps virus
b. Influenza virus
c. parainfluenza virus
d. measles virus
parainfluenza virus
Which can determine the viral load in a COVID-19 patient?
a. electron microcopy
b. in-situ hybridization
c. Real-time PCR
d. FISH
Real-time PCR
The primary infection of HSV-1 infection is seen as
a. Vesicles
b. Macules
c. Papules
d. All of the above.
Vesicles
Koplik spots are seen in infection by
a. mumps virus
b. rubella virus
c. Measles virus
d. parainfluenza virus
Measles virus
Which is true about collection, handling and transport of clinical specimen for
diagnosis of viral disease?
a. The tentative diagnosis of the requesting physician is important to the
laboratory.
b. Tissue samples must be transported in sodium hydroxide solution.
c. The identity of the patient should not be given for data privacy
purposes
d. Specimen is best obtained at the second week of illness
The tentative diagnosis of the requesting physician is important to the laboratory.
Each of the following viruses possess double-stranded DNA as its genome
EXCEPT
a. Measles virus
b. Smallpox virus
c. Epstein-Barr virus
d. Chickenpox virus
Measles virus
Which of the following is implicated together with EBV as a cause infectious
mononucleosis?
a. CMV
b. HSV-1
c. Variola virus
d. VZV
CMV
Which antibody, when present in the serum of a newborn, indicates transfer
of maternal antibody and is protective?
a. IgG
b. IgE
c. IgM
d. IgA
a. IgG
Which of the following can be seen under the light microscope?
a. viral antibody
b. CPE on infected cells
c. Viral particle
d. viral antigen
CPE on infected cells
Which is derived from embryonic tissues?
a. secondary cell culture
b. continuous cell culture
c. Primary cell culture
d. Shell vial culture
secondary cell culture
Pandemics and epidemics of influenza are associated with one of the following
a. genetic reassortment
b. antigenic drift
c. spread by mosquito vectors \
d. changes in RNP
a. genetic reassortment
The last known case of smallpox was seen in
a. 1975
b. 1979
c. 1977
d. 1960
1977
Which should NOT be used to obtain swabs for viral diagnosis?
a. Cotton
b. Ca alginate
c. Nylon
d. Dacron
Ca alginate
Which has the H and N antigens on separate glycoprotein spikes?
a. parainfluenza virus
b. Mumps virus
c. Influenza virus
d. Measles virus
Influenza virus
Detection of specific antibodies against EBV uses
a. Viral capsid Ag (VCA)
b. Vesicular fluid
c. Horse RBC
d. Sheep RBC
Viral capsid Ag (VCA)
Which has the LEAST life span?
a. semi-continuous cell culture
b. primary cell culture
c. continuous cell line
d. diploid cell line
primary cell culture
Which is transmissible through the placental barrier?
a. rubella virus
b. mumps virus
c. Measles virus
d. parainfluenza virus
rubella virus
The following have available vaccine in the form of live attenuated virus
EXCEPT
a. Measles
b. rubella
c. RSV
d. mumps
RSV
Based on its structure, which family of viruses is most likely transmitted by
organ transplant or blood transfusion?
a. Picornaviridae
b. Papillomaviridae
c. Retroviridae
d. Parvoviridae
Retroviridae
To visualize the morphology of influenza virus, which is the best microscope
to use?
a. fluorescent
b. electron
c. Light
d. phase contrast
electron
Which has complex symmetry?
a. Orthomyxovirus
b. EBV
c. VZV
d. Smallpox virus
Smallpox virus
Paul Bunnel test will diagnose
a. VZV infection
b. EBV infection
c. CMV infection
d. Smallpox infection
EBV infection
What is the most important requirement for a virus to multiply in culture?
a. blood
b. cells
c. a protein
d. DNA polymerase
cells
Which step in viral multiplication involves fusion of viral envelope to the host
cell membrane?
a. uncoating
b. release
c. penetration
d. Adsorption
penetration
Viruses that do not exhibit CPE in cell culture may be detected by
a. appearance of vacuoles in cells
b. appearance of syncytia
c. hemagglutination
d. Hemolysis
hemagglutination
A young female presents with large pustules over her face, arms, and palms.
All pustules were about the same size and at about the same developmental
stage. Tests for varicella and herpes simplex were negative. Examination of
exfoliated cells in the pustules would show
a. Multinucleated cells with owl s eye inclusions
b. Multinucleated cells with cytoplasmic inclusions
c. Cells with both nuclear and cytoplasmic inclusions
d. Multinucleated cells with nuclear inclusions
Multinucleated cells with cytoplasmic inclusions
DNA probes are
a. used to detect viral antigens
b. used to prime DNA synthesis in PCR
c. metallic tips connected to a machine and are moved to locate the
presence of any DNA
d. known sequence of DNA that is complementary to target DNA
known sequence of DNA that is complementary to
target DNA
The most rapid definitive method of diagnosis of a genital herpes simplex
infection in a 20-year old man is
a. Direct immunofluorescence test for viral antigen in vesicle fluid.
b. Titer of serum or seminal fluid for antibodies to herpes simplex
c. Detection of anti-herpes simplex antibody in seminal fluid
d. Cell culture of vesicle fluid
Direct immunofluorescence test for viral antigen in vesicle fluid
Which uses its genome as mRNA?
a. Orthomyxoviridae
b. Hepadnaviridae
c. Filoviridae
d. Picornaviridae
Picornaviridae
Which is LEAST accurate concerning herpes simplex?
a. Cold sores are recurrences of genital herpes
b. Herpetic whitlow may be caused by HSV-2
c. HSV-1 more occurs in young children than in adults
d. Immunodeficiency state triggers reactivation of latent infections
Cold sores are recurrences of genital herpes
Which multiplies in the nucleus?
a. Picornaviridae
b. Filoviridae
c. Rhabdoviridae
d. Herpesviridae
Herpesviridae
Which of the following will NOT support the growth of viruses in culture?
a. organ culture
b. enriched agar medium
c. Animals
d. chick embryo
enriched agar medium
The presence of virus growth in culture is indicated by the appearance of
a. Colonies
b. ballooning of cells
c. Growth in 24 hours
d. all of the above
b. ballooning of cells
To detect HSV antigen directly in cells of a vesicular lesion, which would you
use?
a. Isolation using shell vial culture
b. ELISA
c. immunofluorescence technique
d. PCR
immunofluorescence technique
Which has ds-RNA?
a. Reoviridae
b. Parvoviridae
c. Bunyaviridae
d. Retroviridae
Reoviridae
Guarnieri bodies are seen in infections caused by
a. Variola virus
b. VZV
c. HSV-1
d. HSV-2
Variola virus
Burkitts lymphoma is caused by
a. EBV
b. HSV-2
c. Measles virus
d. CMV
EBV
A young female presents with large pustules over her face, arms, and palms.
All pustules were about the same size and at about the same developmental
stage. Tests for varicella and herpes simplex were negative. The physical
characteristics of the causative virus consists of
a. ds-RNA, helical, in segments and no envelope
b. ds-DNA, icosahedral, with envelope
c. ss-DNA and no envelope
d. ds-DNA, complex, with envelope
ds-DNA, complex, with envelope
Which infects the salivary glands, testes, ovary, pancreas and meninges?
a. rubella virus
b. mumps virus
c. parainfluenza virus
d. Measles virus
mumps virus
At which step of viral multiplication does a virus acquire its envelope?
a. Penetration
b. Synthetic phase
c. release
d. assembly
release