MYCOLOGY AND VIROLOGY Flashcards
Members of the plant kingdom
Lack of roots and stems
Lack chlorophyll
Fungi
Coenocytic hyphae
Aseptate hyphae
Type of mycoses produce no cellular response by the host
Superficial mycoses
Arthroconidia on the outside of the hair as well hyphae within the hair shaft
Ectothrix
True yeast produce what in the asexual stage
Blastoconidia
Septate hyphae have
Divisions
Molds with aseptate hyphae produced a specialized hyphal structure called
Sporangiospore
Basic, branching, intertwining structures of molds
Mycelium
Arthroconidia are formed
directly from hyphae by fragmentation
Mycelia within the colony that grow into the substrate are called
Vegetative hyphae
Chlamydospores that grow within the hyphae
Intercalary
Budding forms in which the daughter cell forms as a bud from a single mother cell or forms laterally from a mycelium or pseudo mycelium
Blastospores
Result of asexual production
Ascospores
Conidia are
Asexual spores
Macroconidia are usually
Multicellular
Swollen portion of the conidiophore is called
Vesicle
NOT apply to true yeast
They exhibit thermal dimorphism
Conidia with rough spiny surfaces are described as
Echinulate
Conidia in chains are said to be
Catenate
When preparing temporary mounts of fungal elements, why is the slide gently heated?
Increase the rate of clearing
Acts as a clearing agent to eliminate debris and make fungal elements more prominent in skin, hair, nails
10% KOH
Stain in Aman medium
Cotton blue
In the Periodic Acid Schiff stain (PAS), what colors denote the presence of GLYCOGEN
Red to violet
Stain used for delineation of fungal elements by fluorescent microscopy
Calcofluor white
When the fungus wall is invinsible with the Hematoxylin and Eosin stain, which of the following may be used to restain the slide
Periodic acid Schiff stain
Color of FUNGAL elements in acridine orange staining procedure
Fluoresces a green color
How does Mycosel medium differ from Sabouraud dextrose agar
Contains chlorampenicol and cycloheximide
Fungus medium used for the primary isolation and maintainance of fungal cultures
Sabouraud Dextrose Agar
Indcator in Dermatophyte Test Medium
Phenol Red
If 1% glucose is added to cornmeal agar, Trichopython rubrum can be differentiated from Trichophyton mentagrophytes by the production of
Pigment
Fungi shoul be incubated at
25-30 C
How long should fungus cultures be held before reporting as negative
30 days
Antibiotics added to media for the isolation of pathogenic fungi from contaminated material
Cycloheximide and chlorampenicol
Media stimulates the production of chlamydospores
Cornmeal agar
Media identifies species of aspergillus
Czapek’s agar
Positive resul in the hair baiting test
V-shaped penetration of the hair shaft
Tinea infection is commonly referred to as
Ringworm
Organism that cause white piedra
Trichosporon beigelii
Tinea unguinum is a dermatophyte infection of the
Nail plates
The favus type of Tinea capitis is caused by
Trichophyton schoenleinii
Anthrophilic
Microsporon audouni
Hairs infected with which of these dermatophytes fluoresce under Wood’s lamp
Microsporum canis
Colonization by dermatophytic fungi of the hair, nails and skin is called
Dermatophytoses
Fungi that cause dermatophytoses
Epidermophyton
Trichophyton
Microsporum
Produces macroconidia that are large, multicellular and club shaped with smooth walls
Epidermophyton floccosum
NOT true
T. rubrum is noted for its balloon forms
Produces small, vey slow growing colonies and has favic chandeliers
Trichophyton schoenleinii
Diagnostic for chromoblastomycosis
Sclerotic body
Found in yeast phase of Sporothrix schenckii on culture medium at 37 C
Cigar shaped yeast cells
Result of an antigen-antibody reaction in cases of sporotrichosis
Asteroid body