Mycology Flashcards
Yeast
Unicellular, round, asex reproduce with budding.
Moist colonies, pseudohyphae with constrictions
Mold
Filamentous, tubular branching hyphae with septations
Form fuzzy, velvety or smooth colonies
Mycelium
Large intertwined mass hyphae without reproductive capacity. Diff types: spiral, pectinate, nodular, racquet, favic
Tinea nigra
Hortaea werneckii
Darker peripheral border
Black piedra
PIedra hortae, hard/firm, black nits–adhere to hair
White piedra
Trichosporon beigelii (Ovoides, inkin, asahii, cutaneum), soft, non firm, white, less adherent
Most common tinea capitis in US
T. tonsurans (2nd–T. canis)
Fluorescent Ectothrix
Cats and Dogs Fight and Growl Sometimes
M. canis, audouinii, distrotium, ferrugineum, (T. gypseum, schoenleinii)
Endothrix
Black dot ringworm: Ringo Gave Yoko Two Squeaky Violins
T. rubrum, gourvilli, yaounde, tonsurans, Soudanense, violaceum
Nonflourescent Ectothrix
My Right Nail Gave Me Warts
T. mentag, rubrum, M. nanuum, T. gypseum, T. megninii, verrucosum
Favus/Scutula
T. schoenleinii, violaceum, gypseum
Kerion
M. canis, T. tonsurans, T verrucosum, T. mentag
Treat with pred and anti fungal
Tinea corporis
#1--T. rubrum #2-- T. mentag then canis and tonsurans/gypseum
Majocchi’s granuloma
1-T. rubrum
or T. mentag
–Needs bx and oral anti fungal. Dermatophyte in hair follicle
Tinea barbae–superficial
T. violaceum or T. rubrum
All barbaes need oral anti fungal
Tinea barbae-abscess
M. canis
Tinea barbae–verrucous granulomatosis
E. floccosum
Tinea barbae=-Deep, inflammatory
T. mentag or T verrucosum
T. manuum
T. rubrum, T. mentag, E. floccosum
T. pedis
Most are T. rub rum
Bullous T. pedis
T. mentag
White superficial onychomycosis
T. mentagrophytes (HIV T. rub), also Aspergillus, Acremonium, fusarium, scopulariopsis. Chalky white.
Proximal white sublingual onychomycosis
T. rubrum, near lunula. Marker HIV
Distal lateral sunungual onychomycosis
T. rubrum
Epidermophyton
No Microconidia
Macrocon: club shaped, smooth. Thin and thick walls, 2-6 cells
No hair involve
Trichophyton
Microcon diagnostic
Macrocon: not diagnostic. pencil shaped, smooth
Nonfluoresc ecto and endo invasion
Microsporum
Macro:diagnostic rough walled, barrel shaped
Fluor and non fluor ectothrix invasion
Epidermophyton floccosum
T. cruris and T. pedis; occ corporis/onycho
Microsporum audouinii
T. corporis and capitis
Mouse fur colony
Polished rice –
Microsporum canis
Most common cause T. capitis worldwide
White, fur like with yellow periphery
Polished rice +
Microsporum ferrugineum
T. capitis and corporis
Smooth, waxy, rust colored, heaped
Microsporum gallinae
Rare cause tinea capitis, usually chicken favus
Fluffy to felt-like what colony becoming pink
M. gypseum
Tinea corporis
Flat, powdery, buff to cinnamon with white periphery
Polished rice +
M. nanum
Tinea capitis
Fluffy white, powdery baige
Pig snout macroconidia
In Vitro Hair Perforation
+wedge shaped area in hair shaft–T mentag
no change in hair shaft–T rub rum, ferrug
Pigmentation Production on cornmeal agar with dextrose
+ T rubrum
-T mentag
Urease Test
+red pink–T mentag
-amber T rubrum, ferruge, soudanese
Trichophyton equinum
Requires niacin for growth
cream to yellow; fluffy with radial grooves
Trichophyton menginii
T. barbae, T. capitis, corporis, unguium
Requires histidine
white to pink suede-like colony flat or with gentle folds
Trichophyton mentagrophytes
var. interdigitale-anthro
var. mentagro-zoophilic
brown to red
Spiral hyphae
Hair perf+, urease +, pigment -
Trichophyton rubrum
One hand, 2 foot birds on a wire Pigment on agar + Urease - Hair perfor -
Trichophyton soudanense
T. capitis, corporis
Urease negative
Trichophyton tonsurans
Most common cause T capitis in US
BLack dot endothrix
Partial thiamine requirement
Trichophyton concentricum
Tinea imbricata
Deeply folded, cerebriform
Some need thiamine
Trichophyton schloenleinii
Capitis, corporis, unguium
Favic chandelier
Trichophyton verrucosum
Ecto capitis, Inflamm barbae, T. corporis, faciei
Grows best at 37
Needs Thiamine +- inositol
Waxy white heaped
Trichophyton violaceum
T. capitis
Purple colony
Partial need for thiamine
Scytalidium dimidiatum
Soles, toe webs. PIgmented hyphae
Sesnsitive to cycloheximide
Resistant to antifungals
E. floccosum
T. cruris and pedis
M. audouinii
Polish rice -
Former #1 tinea capitis
M. canis
#1 tinea capitis world wide Polish rice+
T. rubrum
1 worldwide overall
T. tonsurans
1 t capitis in US
Most common fungus in burn patients
Fusarium
Fusarium
Hyalohyphomycosis
Histo looks like aspergillosis
Cause painful cellulitis, abcess, necrotic nodules
Banana shaped
Penicillium
Blue grey colony with white border
External ear, mycotic kertitis
Scopulariopis
White superficial onychomycosis
Paecilomyces
Erythematous macules, vesicles, pustules or nodules
Phaeohyphomycosis
Fontana Masson
Alternaria
Phaeohyphomycosis
Common plant pathogens
Looks like hand grenade
Cryptococcus
Encapsulated yeast
Pigeon droppings
Stain: PAS, GMS, Fontana Masson
Mucicarmine or alcian blue capsule
Rhinosporidium seeberi
Large polyps, wartlike lesions in nasal mucosa, eye, mouth.
Red with mucicarmine
Protothecosis
algae
Olecranon bursitis in 1/3 of cases
Spiral hyphae Mycelia
T. mentagrophytes
Pectinate bodies mycelia
Look like comb-M. audouinii
Nodular bodies
Knot of twisted hyphae
Racquet hyphae
Club shaped–C. immitis
Favic chandeliers
Antlers–T. schoenleinii
Arthroconidia–Asexual conidia
C. immitis-barrel shaped spores released by fragmentation
Chlamydoconidia–Asexual conidia
C. albicans, T. tonsurans–Spherical, resist to environment
Blastoconidia–Asexual conidia
Yeast–formed by budding
Sporangia–Asexual conidia
Spores produced in a sac–Zygomycetes
Conidia–Asexual conidia
Cells made on end of sides of hyphae–Dermatophyte
Trichosporanosis
Via. T. asahii–fungemia in immcompromised patient
Tinea imbricata
T. concentricum
Tinea profunda
Often verrucous.
tinea cruris
E. flocossum, T. rubrum or T. mentag
tinea facei
Usually child upper lip and chin after animal exposure
T rubrum, T mentag, M. canis or T. concentricum
Mocassin T pedis
T. rubrum, E. floccosum
Bullous T pedis
T. mentagrophytes
Interdigital T pedis
T. rubrum, T. mentag (E. floccosum)
Onychomycosis
T. rubrum
Candida glabrata
#2 - 15-20% patients with invasive candida Fluconazole resist
Candida parapsilosis
chronic paronychia and systemic disease
Candida tropicalis
septicemia esp in leuk, lymphoma and DM
Candida dubliniensis
Oropharn of HIV patients and often after prev treatment with antifungals
Chromoblastomycosis
Fonsecaea pedrosoi (most common) Fonsecaea compacta Rhinocladeilla aquaspersa Phialophora verrucosa Exophiala jeanselmei Cladophialophora carrionii
Hyalohyphomycosis
Nondematiaceous, hyaline fungi
Penicillium, Acremonium, Fusarium, Scopulariopsis
Banana shaped macroconidia
Phaeohyphomycosis
Dematiaceous Alternaria--hand grenades Curvularia Exophiala Biopolaris
Rhinosporidiosis
Rhinosporidium seeberi, water protozoan
GIANT SPORANGIA (up to 500um)
Nasal mucosa–vegetating polyps
Aspergillosis
A. flavus-yellow to green culture. Most common for cutaneous disease
A. fumigatus-most com species
A. niger
Branch at 45deg
Sabouraud Dextrose Agar
Gold Standard. Nutritionally poor.
Peptone, glucose, water, agar
Modified SDA
Mycosel or Mycobiotic.
SDA+cycloheximide+chloramphenicol
Good for hair, nails, skin.
Cyclohex inh’s saprophytic fungi (protothecosis, H. werneckii, scytalidium, candida other than albicans, C neoformans)
Dermatophyte Test Media
Peptones, dextrose, cycloheximide, phenol red, chlortetracycline, gentamicin
Dermatophytes–turn from amber to RED bc alkaline by products
Nondermato-cause media to turn yellow or stay amber bc of acid