Mycobacterium

Question 2

Mycolic acid in cell wall bacteriaa

Answer 2

CMN
Corynebacyeria
Mycobacteria
Nocardia

Question 3

Atypical mycobacterium causes disease mostly in whom

Answer 3

In deficient cell mediated immunity

Question 4

Cultivation of atypical mycobacterium

Answer 4

LJ medium

Question 5

Kunyons classification of atypical mycobacteria

Answer 5

Photochromogens (pigment in light) Simi, Kansas , asia

Scotochromogens (pigment in dark and light) szulgai, scrofulaceum, gordon

Non photochromogens ( no pigment ) ulcerans, xenopi, gena

Rapid growers
Abscessus, chelonei

Question 6

M leprea acid fast with ______ h2so2

Answer 6

5%

Question 7

M leprea
Obligated intracellular
Grows on

Answer 7

Foot pad of mice
Armadillos
Indian pangolin

Question 8

Diagnosis and screening of mtb

Answer 8

1. Microscopy
   ZN stain and kinyoun stain
   Fluorescent dyes( auramine o rhodamine b)
2. Homogenisation and decontamination methods
   Petroffs ( 4 or 2% naoh + n acetyl n cysteine)
3. Culture
   Gold standard

Solid culture
a) egg : LJ , dorset egg petragnani
b) blood
c) serum ( leoffler)
d) potato
e) agar

Liquid culture
Middle brook 7H9, 7H12
Serpentine cords of growth

4. Automated
   BACTEC mgit
   BactAlert
5. Mycobacteriophage
6. Molecular
   PCr elisa
7. Antibiotic sensitivity
   LJ medium
   Liquid MODS
   Molecular Xpert , pcr

Question 9

Disc diffusion is not used for dwhich bacterial antibiotic sensitivity

Answer 9

MTB

Question 10

Screening tests

Answer 10

1. Mantoux /tuberculin

+Ve in BCG vaccinated patients

2. IGRA :
   Quantiferon gold in tube
   T spot TB
3. MPB64 skin/ transdermal patch test
   Dx of active tb