Bacillus

Question 1

__________ flagella, except for

Answer 1

pEritrichous flagella except B.anthracis

Question 2

Spores : type and formation

Answer 2

Non bulging spores
Formed in soil and culture Never formed in human body

Question 3

Bacillus anthracis
-Capsule property
- Appearance
- Grows in _______
- Sucetiple to ____ phage
- gelatin medium

Answer 3

• McFaydens reaction (polychrome methylene blue)
• Bamboo stick
• chains
• gamma
• inverted fir tree appearance in gelatin

Question 4

Colony morphology

Selective medium

Answer 4

• Medussa head /comet tail/ whipped egg white appearance
• Selective medium : PLET medium

Question 5

Virulence

Answer 5

Capsule ( antiphagocytic)
Anthrax toxin

Question 6

Tripartite toxin

Answer 6

3 components

A. Protective antigen : Facilitates entry of EF and LF
B. EF edema factor : increases cAMP –> cell edema
C. LF Lethal factor : inhibits MAPKKs —–> cell death

Question 7

Vccine

Answer 7

Anthrax vaccine adsorbed (AVA)
-Contains protective antigen
-Sterne strain

Question 8

Anthrax

Transmission
Types

Answer 8

Transmission:
Inhalation
Ingestion : Gastrointestinal
Cutaneous : through skin

Types
1. Cutaneous : malignant pustule /hide porters
2. Inhalational : wool sortes
3. GI : Oropharyngeal ulcer and psudomembrane, hemorrhagic enteritis

Question 9

Diagnosis

Answer 9

Samples : swaba, sputum, CSF, stool, rectal swab
Gram stain : Gram positive rods
Antigen : PCR , culture