Streptococcus Flashcards
Detection of catalase enzyme is by
Addition of H2O2 ( hydrogen peroxide)
Gram positive cocci in
clusters
Chains
Pairs
Clusters : Staph aureus
Chains : beta (s.pyogemes)
Pairs : alpha and gamma (Pneumococcus, Enterococcus)
Hemolysis
Alpha, beta , gamma cocci
Alpha: S.pneumoniea, viridans
Beta : S.pyogenes, S.agalctiae
Gamma: Enterococcus
M/c cause of dental caries
SABE
S.mutans
S. Sanguis
S.penumococcus
Type 1-8
Type 6,14,19,23 causes which disease
Type 1-8 : pneumococcal infection in adults
Type 6,14,19,23 : Pneumococcal infection in children
Which is most virulent s.pneumococci
Type 3
Capnophilic bacteris
S.pneumoniea
H.influenza
Nisseria
(SHIN)
M/c cause of
Otitis media
Sinusitis
S.penumoniea
M/c of meningitis in
1. Adults
2. Adolescents
3. Neonates
- S.pneumoniea
- Meningococcus> pneumo
- E.coli
Endocarditis in IV drug users
Enterococcus (gamma)
Beta hemolytic cocci is classified based on
C carbohydrate in cell wall
GABHS and GBBHS example
- S.pyogenes
- S.agalcteia
S.pyogenes
Capsule Made of
Cell wall contaisn
- Hyaluronic acid
- M PROTEIN
M1, m2,m3 causes
M49,m 57, m59
Respiratory tract infection
Skin and soft tissue (impetigo)
Common for all streptococcus
Catalase _____
Oxidase ______
________ anerobes
Catalase negative
Oxidase negative
Facultative anaerobes
Gram positive cocci in
- Grape like cluster
- Chains
- Pairs (lanceolate/flame)
- Staph aureus
- Strep pyogenes(beta)
- Pneumococcus(alpha) Enterococcus(gamma)
Alpha. Beta. Gamma
Alpha : S.pnuemo, Viridans Beta: S.pyo, S.galactiea
Gamma : Enterococcus
S.pyogenes
1. Sensitive for ________
2. A specific test
3. Pyr ____
- Bacitracin sensitive
- Ribose fermentation negative
- Pyr +ve
S pyogenes on blood agar ______________
Based on appearance of colonies
Matte :
Mucoid:
Glossy :
Pin point colonies with wide zone of beta hemolysis
Matte and mucoid : M protein +ve, capsule +ve (virulent)
Glossy : M protein -ve, non capsulated (avirulent)
Transport medium for s.pyogenes
Selective medium?
Pikes medium
Crystal violet blood agar
Secreted by S pyogenes
( S M A S H E D)
A. Streptolysin ( O and S)
B. Streptokinase : lysis fibrin, spread of infection
C. Streptordonase : depolymerises DNA (D)
D. Superantigens(S)
E. Hyaluronidase(H)
F. Erythrogenic toxins(E)
G. C5a peptidase (A)
H. M protein (M)
Toxins used in
1. Rheumatic fever
2. MI
3. PSG nephritis
- Anti ASO antibodies (induced by streptolysin O)
- Streptokinase (fibrinolysis)
- DNAse /streptordonase
M/c bacterial cause of pharyngitis
S.pyogenes