MUST KNOW URINALYSIS Flashcards
pigment that gives yellow color to the urine
urochrome
pink pigment in refrigerated specimens
uroerythrin
oxidation product of urobilinogen
urobilin
urine color significance:
colorless
recent fluid consumption
urine color significance:
pale yellow
diluted urine, DI/DM
urine color significance:
dark yellow
concentrated, dehydration, bilirubin
urine color significance:
orange yellow
phenazopyridine
urine color significance:
yellow green
biliverdin
urine color significance:
green
Pseudomonas infection
urine color significance:
blue-green
indican
urine color significance:
pink
RBCs (cloudy)
urine color significance:
red
hemoglobin, myoglobin (clear)
beets, rifampin
urine color significance:
port wine
porphyrin
urine color significance:
red brown
methemoglobin, myoglobin
urine color significance:
brown
homogentisic acid
urine color significance:
black
melanin/melanogen
urine odor significance:
aromatic
normal urine
urine odor significance:
ammoniacal
UTI
urine odor significance:
fruity, sweet
ketonuria, dehydration, DM
urine odor significance:
maple syrup
maple syrup urine disease
urine odor significance:
mousy
phenylketonuria
urine odor significance:
rancid
tyrosinemia
urine odor significance:
sweaty feet
isovaleric anemia
urine odor significance:
cabbage
methionine malabsorption
urine strip 30 secs:
bilirubin, glucose
urine strip 40 secs:
ketones
urine strip 45 secs:
specific gravity
urine strip 60 secs:
pH blood protein nitrite urobilinogen
urine strip 120 secs:
leukocyte esterase
bilirubin principle
diazo reaction
other bilirubin test
icto test (purple)
bilirubin significance
hepatitis, jaundice
glucose principle
double sequential enzyme reaction
glucose oxidase + peroxidase
other glucose test
benedict’s test (blue, green, orange, red)
glucose significance
DM, GD
ketone principle
sodium nitroprusside
other ketone test
acetest (purple)
ketone principle
DKA, dehydration
specific gravity principle
change in pKa of polyelectrolyte in an alkaline medium
other specific gravity test
urinometer, refractometer
pH principle
double indicator (methyl red and bromothymol blue)
protein principle
protein error of indicator
other protein test
sulfosalicylic acid test
protein significance
prerenal, renal, postrenal proteinuria
blood principle
pseudoperoxidase activity of hemoglobin
test to differentiate blood and myoglobin
blondheim’s test
(ammonium sulfate)
hgb - ppt
mgb - supernatant
blood significance
intravascular hemolysis, glomerular damage, bleeding, stones
nitrite principle
Greiss reaction (diazo)
nitrite significance
UTI
urobilinogen principle
ehrlich’s reaction
test to differentiate urobilinogen and other ehrlich-reactive substance
watson-schwartz test
urobilinogen: soluble on chloroform and butanol
urobilinogen significance
liver disease, hemolytic disorders
leukocyte esterase principle
action of leukocyte esterase
substances that are positive on leukocyte esterase (3)
granulocytes
trichomonas
histiocytes
leukocyte esterase significance
UTI
RBC shape:
hypersthenuric urine
crenated
RBC shape:
hyposthenuric urine
ghost cells
RBC shape:
glomerular bleeding
dysmorphic
RBC normal value in urine
0-2/hpf
WBC normal value
0-5/hpf
pyuria value
> 8/hpf
most significant epithelial cell in the urine
renal tubular epithelial cell
RTE cell type seen in nephrotic syndrome
oval fat bodies
parasite in urine
Trichomonas vaginalis
Schistosoma haematobium
where are casts formed?
between DCT and CD
casts are made up of what type of protein?
tamm-horsfall protein/uromodulin
what cells produce uromodulin?
RTE cells
beginning of all casts
hyaline cast
pyelonephritis vs cystitis vs inflammation
pyelonephritis:
w/ WBC cast
w/ WBC and Bx
cystitis:
w/o WBC cast
w/ WBC and Bx
inflammation
w/ WBC cast
w/ WBC
w/o Bx
aka ‘renal failure cast’
broad cast
3 factors that affect crystal formation in urine
pH
temperature
solute concentration
acidic urine crystals (5)
- amorphous urates
- uric acid
- sodium urates
- calcium oxalate dihydrate
- calcium oxalate monohydrate
alkaline urine crystals (5)
- ammonium phosphates
- triple phosphate
- calcium phosphate
- calcium carbonate
- ammonium biurate
abnormal urine crystals (6)
cystine cholesterol radiographic dyes tyrosine leucine bilirubin
liver disease crystals (3)
tyrosine
bilirubin
leucine
abnormal crystals due to medication (2)
- sulfonamide
- ampicillin