MT3 Session 14:dsDNA Animal viruses

Question 1

serial innoculation: +/-

good alternative

Answer 1

make viruses by infecting healthy animals
+ meaintains original virulence
- takes time, money

good alt = tissue culture

Question 2

animal viruses vs. phages

Answer 2

replication time longer, burst size larger

Question 3

plaque isolation and assay of ani viruses for lysers

Answer 3

1. infect monolayer of ani cells with virus
2. remove liquid, add gelatin
   higher viscosity will concentrate viruses into plaquesat lysis site

Question 4

plaque isolation and assay of ani viruses for non-lysers

Answer 4

1. fluorescent focus assay- make viruses have GFP

transformed focus assay- for oncogenic viruses

Question 5

virus classification- group 1

Answer 5

dsDNA

Question 6

nonenveloped dsDNA viruses - dna shape

Answer 6

papilomavirus - circular, adenovirus-linear

Question 7

HPV symptoms

Answer 7

warts

cercival cancer, cancer

Question 8

HPV structure

Answer 8

icosahedral made of pentamers

Question 9

HPV live cycle (large scale)

cancery

Answer 9

1. infects basal skin cells
2. virus is activated when cells differentiate
3. shedding releases virons

vancer if HPV integrates into host

Question 10

hpv life cycle (inside cell - small scale)

Answer 10

1. attachment - engulf whole
2. virus reaches nucleus and inserts DNA
3. replicate dna and capsid
4. transport capsid into nucleus
5. assemble parts, leave nucleus

Question 11

biochem of warts

Answer 11

E7 causes pRb to be proteased
pRb can’t make cell division regulators

so uncontroleld cell division occurs

Question 12

L1,L2

Answer 12

HPV capsids are made of these 2

Question 13

VLP

Answer 13

virus like particle

has L1 and L2’s like hpv to cause immunity

Question 14

adenovirus disease

Answer 14

respiratory syndromes

eye/GI infections

Question 15

CAR

Answer 15

coxsackie and adenovirus receptor

on host

Question 16

adenovirus size

Answer 16

HUGE

Question 17

ADENOVIRUS GENES

Answer 17

can read both directions -> HUGE

Question 18

characteristic (physical) of adenovirus

Answer 18

fiber spikes

hexon pieces fill penton apeces

Question 19

how did we discover mRNA splicing?

Answer 19

adenovirus

Question 20

normal fxn of icam?

abnormal fxn?

Answer 20

pulls wbc’s pass the blood vessel

attachment site for rhinovirus

Question 21

NTR of poliovirus

Answer 21

includes IRES (internal ribosome entry site_) to bind rbosome

Question 22

polliovirus capsid assembly/synthess

Answer 22

makes 1 linear strand

folds into a 2d shape

Question 23

poliovirus attachment

Answer 23

endocytosis

CD155 pinches virus -> VPg takes RNA

Question 24

poliovirus attachment on host

Answer 24

CD155