MS- overview

Question 1

What is MS?

Answer 1

A technique used to measure Mr of molecules

Question 2

Does MS device produce and weigh ions?

Answer 2

YES

Question 3

Uses of MS?

Answer 3

ID of drugs
Confirm presence
Quality control of recombinant proteins
ID of unknown drug metabolites

Question 4

Does MS help in drug discovery?

Answer 4

YES- helps in sequencing of proteins

Question 5

X and Y axis of MS graph?

Answer 5

X= m/z (mass:charge) 
Y= abundance in %

Question 6

The most abundant ion is the highest on the Y axis (100%) -T/F?

Answer 6

TRUE

Question 7

Molecules in the sample are Identified according to their…?

Answer 7

MASS

Question 8

What are the Major steps of MS?

Answer 8

1- sample vaporisation
2- ion generation
3- ion separation according to M/Z
4- ion detection

Question 9

3 ways sample volatilisation can be achieved?

Answer 9

1- heat
2- placing in vacuum
3- fast atom bombardment

Question 10

Why sample volatilisation?

Answer 10

Gives even distribution of molecules in vacuum chamber

Question 11

How is sample ionisation done?

Answer 11

By bombarding volatilised molecules with E- from E- gun

Leads to molecular ionisation