Molecular Diagnostic Techniques

Question 1

Electrophoresis can be defined as the -?

Answer 1

Separation of molecules based on charge and size along an electrophoretic field

Question 2

___________ ________ cut DNA at specific sequences like the EcoRI forming a sticky end and Smal making a blunt end.

Answer 2

Restriction enzymes

Question 3

When a probe binds to its complementary sequence along DNA, this is called -?

What is a probe?

Answer 3

Hybridization

A probe is a fragment of nuclei acids that can be illuminated for detection and have a high degree of specificity

Question 4

T/F: Hybridization probes can be used in DNA or RNA samples to detect the presence of nucleotide substances that are complementary to the sequence in the probe.

Answer 4

True

Question 5

Define target amplification.

Answer 5

An enzyme mediated process that synthesizes copies of targeted nucleic acids.

Question 6

What are the following steps of PCR:

1. Heating that leads to the separation of DNA
2. The cooling of DNA to allow for a reformation of hydrogen bonds
3. The addition of nucleotides to the 3’ end of DNA at the end of each primer

Answer 6

1. Denaturation
2. Annealing
3. Extension

Question 7

Primer are associated with being _______ __________ DNA fragments that bind to specific sites along DNA determining the size of the PCR product.

They can be -?

Answer 7

Single Stranded

Random, specific, and vary in nucleotide composition

Question 8

Real Time PCR involves a __________ signal.

Answer 8

Fluorescent