Module 6 Lesson 1 Flashcards
Total aerobic count medium
Broth and agar
types of broth and agar for total aerobic count
Broth :
Tryptone soya broth, soybean-casein digest broth, Trypticon soy broth
Agar:
Tryptone soya agar, soybean-casein digest agar, Trypticon soy agar
Setting for total aerobic count
30 to 25 C for 18 to 24 hrs
Microbial limit test methods
Total aerobic count
Total combined yeast and mold count
Medium for total combined yeast and mold count
Potato dextrose agar, broth saboraud dextrose agar/broth
Setting for total combined yeast and mold count
20 to 25 C for 2-3 days
Liquid dosage forms
Solutions
Emulsions
suspensions
Solutions
Appearance test
Visual inspection
Check for the presence of impurities
conducted by determining the amount of preparation of unknown potency required to produce a definite effect on Suitable test animals
Biological Assay
amount of force to redisperce particles
redispersibility
measures zone of inhibiton
cylinder plate method
measures turbidity using spectrophotometer
turbidimetric method
cylinder plate method:
Antibiotic:??
test organism: S. aureus
cephalothin, cloxacillin, naficillin, Pen G
cylinder plate method:
Antibiotic:??
test organism: s.cerevisiae
amphotericin B, Nystatin
cylinder plate method:
Antibiotic:??
test organism: micrococcus lutues
bacitracin, eryhthromycin
cylinder plate method:
Antibiotic:??
test organism: bordetekllla bronchiseptica
polymyxin B
cylinder plate method:
Antibiotic:??
test organism:B. subtilis
vancomycin
cylinder plate method:
Antibiotic:??
test organism: s. epidermis
gentamicin, neomycin
cylinder plate method:
Antibiotic:??
test organism: mycobacterium smegmatis
bleomycin
cylinder plate method:
Antibiotic:??
test organism: p. aeruginosa
carbenicillin
turbidimetric method test organism: s. aureus
amikacin, cycloserine, demeclocycline, doxycycline, kanamycin, tetracycline, tobramycin
turbidimetric method test organism: k pneumoniae
neomycin, streptomycin
