module 5

Question 1

All of these components are needed to create and select for a bacteria expressing a human
gene in a plasmid except:
a. Origin of replication
b. Restriction endonuclease site
c. Promoter to drive transcription
d. Eukaryotic DNA polymerase
e. Selectable marker

Answer 1

d. Eukaryotic DNA polymerase

Question 2

Why is it necessary to produce cDNA of Eukaryotic genes for cloning into bacteria for
expression?
a. Bacteria don’t have the mechanisms to produce a poly-A tail
b. Bacteria don’t have the mechanisms to eliminate introns
c. Bacteria don’t have the mechanism to produce a 5’ cap
d. Bacteria don’t have the mechanism to transcribe eukaryotic DNA

Answer 2

b. Bacteria don’t have the mechanisms to eliminate introns

Question 3

What would be the result of performing CRISPR on a gene with the use of a donor DNA?
a. DNA would be cleaved and reattached using non-homologous end joining
b. DNA would be cleaved and reattached using homologous recombination
c. DNA would not be cleaved but homologous recombination would occur
d. DNA would not be cleaved but non-homologous end joining would occur
e. CRISPR would not work

Answer 3

b. DNA would be cleaved and reattached using homologous recombination

Question 4

Somatic nuclear transfer utilizing a skin cell as the donor to clone a whole mammalian
organism is an inefficient process for which of the following reasons?
a. Skin cells only contain DNA associated with skin cell genes
b. The genome of the skin cell is too large
c. Skin cells undergo too many mutations
d. Skin cells don’t have nuclei
e. Chromatin modifications in skin cells

Answer 4

e. Chromatin modifications in skin cells

Question 5

What aspect of human cloning does NOT pose an ethical issue?
a. Cloning requires surrogate mothers
b. Human egg donors are required
c. Human clones will have the memories of the original
d. Cloning is inefficient – only a small percent of SNT is successful
e. All of the above are ethical issues

Answer 5

c. Human clones will have the memories of the original

Question 6

Why are induced pluripotent stem cells potentially more promising for autologous therapies
than other sources of stem cells?
a. No destruction of human embryos
b. The reintroduced stem cells have a low risk of becoming tumorigenic
c. When introduced back into patients they are less likely to generate an immune
response
d. Both A and B
e. Both A and C

Answer 6

e. Both A and C

Question 7

Which of these statements is NOT true of B-cells?
a. All B-cells are genetically identical
b. Plasma cells are antibody producing B-cells
c. B-cells are involved in the humoral immune response
d. Memory B-cells are created in response to 1st antigen exposure

Answer 7

a. All B-cells are genetically identical

Question 8

Which of these cell types is mainly responsible for upregulating and supporting both the
humoral and cell-mediated cell response?
a. Dendritic cells
b. B-cells
c. Helper T-cells
d. Cytotoxic T-cells
e. Erythrocyte

Answer 8

c. Helper T-cells