Module 2.1 - Cell Structure Flashcards
What kind of magnification do microscopes produce and what does this mean?
Linear - if an image is x100 magnification appears to by 100x wider and 100x longer than specimen
What are light microscopes also known as?
Optical microscopes
What are the advantages of light microscopes?
- Relatively cheap
- Easy to use
- Portable as can use in field or laboratories
- Can study living specimens
What is the maximum magnification of a light microscope?
X1500 (x2000 in some)
What is the maximum resolution of a light microscope and why?
200nm, source is visible light with wavelength of 400-700nm, so structures close than 200nm appear as one
What is the equation for magnification?
Magnifying power of objective lens x magnifying power of eyepiece lens
How do laser scanning microscopes work?
- Use laser light to scan an object point by point + assemble pixel info into one image by computer, displayed on a computer screen
- Images are high resolution + show high contrast
- Have depth selectivity + can focus of structures at different depths within a specimen
- Used in the medical profession for a quick diagnosis + earlier/more effective treatment
- Used in many branches of biological research
How do electron microscopes work?
- Use a beam of fast travelling electrons
- Wavelength of about 0.004nm so have a greater resolution to give a clear, highly magnified image. Higher resolution than light microscopes as shorter wavelength
- Electrons fired from a cathode + focused by electromagnets rather than glass lenses onto a screen or photographic plate making a black + white image (electron micrograph)
How do transmission electron microscopes (TEMs) work?
- Specimen has to be chemically fixed by being dehydrated + stained
- Beam of electrons passes through specimen stained with metal salts. Some electrons pass through + are focused on a screen or plate
- Form a 2D grey scale image (electron micrograph)
What is the maximum resolution of a TEM?
0.1nm
What is the maximum magnification of a TEM?
x500000
How does a scanning electron microscope (SEM) work?
- Electrons cause secondary electrons to bounce off the specimen’s surface + be focused on a screen
- Gives a 3D image
- Black + white but a computer can add false colour
What is the maximum resolution of an SEM?
10nm
What is the maximum magnification of a scanning electron microscope?
x100000
What is differential staining?
Stains that bind to specific cell structures, staining each structure differently so the structures can be easily identified within a single preparation
Give an example of an all purpose stain.
Methylene blue
Give some examples of stains used in microscopy and what they stain.
- Acetic orcein: binds to DNA + stains chromosomes dark red
- Eosin: stains cytoplasm; Sudan red stains lipids
- Iodine in potassium iodide solution: stains cellulose in plant cell walls yellow + starch granules blue/black (look violet under a light microscope)
How are specimens prepared for light microscopy?
- Specimen is dehydrated
- Embedded in wax to prevent distortion during slicing
- Use a special instrument to make thin slices called sections. These are stained + mounted in a special chemical to preserve them
What is the equation that links the image size, magnification and the actual size of the specimen?
Magnification = image size / actual size
same units
How do you use a stage graticule to calibrate the eyepiece graticule?
- Insert eyepiece graticule into x10 eyepiece of microscope. Ruler has 100 divisions (0.01mm/10micrometre divisons, 1mm total).
- Place stage microscope on microscope stage + bring into focus using lower power (x4 objective so x40 magnification).
- Align eyepiece graticule + stage graticule. Check value of 1 eyepiece division at this magnification.
- Divide length of the stage graticule (1000 micrometres) by the number of eyepiece units it takes up, giving the division of each epu in micrometres.
- Now do the same for the x10 magnification and x40 magnification (eyepiece).
Give the rough value of each epu in micrometres in most modern microscopes used in schools (rough if asked in exams) when the magnification of the eyepiece lens is x10.
- Mag. of objective lens: x4, total: x40, each epu = 25
- Mag. of objective lens: x10, total: x100, each epu = 10
- Mag. of objective lens: x40, total: x400, each epu = 2.5
- Mag. of objective lens: x100 (oil-immersion lens), total: x1000, each epu = 1.0
Which kingdom’s cells are eukaryotic?
- Animals
- Plants
- Fungi
- Protoctists
What do all eukaryotic cells have?
- Nucleus surrounded by nuclear envelope containing DNA organise + wound into linear chromosomes
- Nucleolus in nucleus containing RNA where chromosomes unwind. Involved in making ribosomes
- Cytoplasm in which organelles are suspended
- Cytoskeleton: network of protein filaments (actin or microtubules) within cytoplasm that move organelles within the cell. Allow some cells (amoebae + lymphocytes) to move. Allow contraction of muscle cells
- Plasma membrane
- Membrane bound organelles e.g. mitochondria, Golgi, ER
- Small vesicles
- Ribosomes where proteins are assembled
What is the structure of the nucleolus?
- Doesn’t have a membrane around it
- Contains RNA
What is the structure of the nucleus?
- Surrounded by a double membrane - nuclear envelope
- Pores in the nuclear envelope
- Contains DNA. Chromatin if the genetic material, consisting of DNA wound around histone proteins. When cell isn’t dividing, chromatin is spread out or extended. When cell is about to divide, chromatin condenses + coils tightly into chromosomes. These make up nearly all the organism’s genome
What is the function of the nuclear envelope?
- Separates contents of nucleus from rest of cell
- In some regions outer + inner membranes fuse together. At these points dissolved substances + ribosomes can pass through
- Pores enable larger substances e.g. messenger RNA (mRNA) to leave nucleus. Substances e.g. steroid hormones, may enter nucleus from cytoplasm via these pores
What is the function of the nucleolus?
Where ribosomes are made
What is the function of the nucleus?
- Control centre of the cell
- Stores organism’s genome (chromosomes contain organism’s genes)
- Transmits genetic information
- Provides instructions for protein synthesis
What is the structure of the rough endoplasmic reticulum (RER)?
- System of membranes, containing fluid-filled cavities (cisternae) continuous with the nuclear membrane
- Coated with ribosomes
What is the function of the rough endoplasmic reticulum?
- Is the intracellular transport system: cisternae form channels for transporting substances from one area of a cell to another
- Provides large SA for ribosomes, which assemble amino acids into proteins. These actively pass through membrane into cisternae + are transported to Golgi apparatus for modification + packaging
What is the structure of the smooth endoplasmic reticulum (SER)?
- System of membranes, containing fluid filled cavities (cisternae) continuous (in line with) nuclear membrane
- No ribosomes on its surface
What is the function of the smooth endoplasmic reticulum?
- Contains enzymes that catalyse reactions involved with lipid metabolism, e.g. synthesis of cholesterol, lipids/phospholipids needed by the cell, or steroid hormones
- Involved with absorption, synthesis + transport of lipids (from gut)
What is the structure of the Golgi apparatus?
- Consists of a stack of membrane-bound flattened sacs
- Secretory vesicles bring materials to + from Golgi apparatus
What is the function of the Golgi apparatus?
- Proteins are modified e.g. adding: sugar molecules to make glycoproteins, lipid molecules to make lipoproteins, + being folded into their 3D shape
- Proteins packages into vesicles that are pinched off then stored in cell or moved into plasma membrane, either to be incorporated into plasma membrane or exported outside the cell
What is the structure of the mitochondria?
- May be spherical, rod-shaped or branched. 2-5μm long
- Surrounded by 2 membranes with a fluid-filled space between them. Inner membrane is folded into cristae
- Inner part of mitochondrion is a fluid filled matrix
What is the function of the mitochondria?
- Site of ATP (energy currency) production during aerobic respiration
- Self-replicating, so can be made if cell’s energy needs increase
- Abundant in cells where much metabolic activity takes place e.g. liver cells + synapses between neurones where neurotransmitter is synthesised + released
What is the structure of chloroplasts?
- Large organelles, 4-10μm long
- Only found in plant cells + some protoctists
- Surrounded by a double membrane. Inner membrane is continuous stacks of flattened membrane sacs, thylakoids (resembling pile of plates) which contain chlorophyll. Each pile of thylakoids called granum (pl. grana). Fluid filled matrix called stroma.
- Grana interconnected by tubular extensions: intergranal lamellae
- Contain loops of DNA + starch grains
What is the function of the chloroplasts?
- Site of photosynthesis
- 1st stage of photosynthesis, when light energy trapped by chlorophyll + used to make ATP, occurs in grana. Water also split to supply hydrogen ions
- 2nd stage, when hydrogen reduces CO2 using energy from ATP, to make carbohydrates, occurs in the stroma. Abundant in leaf cells, particularly in palisade mesophyll layer
What is the structure of vacuoles?
Surrounded by a membrane called tonoplast + contains fluid
What is the function vacuoles?
- Only plant cells have a large permanent vacuole
- Filled with water + solutes + maintains cell stability as when full it pushes against cell wall making cell turgid
- If all plant cells are turgid this helps support plant, especially in non-woody plants
What is the structure of lysosomes?
- Small bags, formed from Golgi apparatus. Each is surrounded by a single membrane
- Contain powerful hydrolytic (digestive) enzymes
- Abundant in phagocytic cells e.g. neutrophils + macrophages (types of WBC) that can ingest + digest invading pathogens e.g bacteria
What is the function of lysosomes?
- Keep powerful hydrolytic enzymes separate from rest of cell
- Can engulf old cell organelles + foreign matter, digest them + return digested components to the cell for reuse
What is the structure of cilia and undulipodia?
- Protrusions from cell + surrounded by the cell surface membrane
- Each contains microtubules
- Formed from centrioles
What is the function of cilia and undulipodia?
- Epithelial cells lining airways each have many hundreds of cilia that beat + move the band of mucus
- Nearly all cell types have one cilium that acts as an antenna. Contains receptors + allows cells to detect signals about its immediate environment
- Only type of human cell that has an undulipodium (longer cilium) is a spermatozoon. Enables it to move
What is the structure of ribosomes?
- Small spherical organelles, about 20nm in diameter
- Made of ribosomal DNA
- Made in nucleolus, as 2 separate subunits, which pass through nuclear envelope into cytoplasm then combine
- Some remain free in cytoplasm + some attach to the ER
What is the function of ribosomes?
- Bound to exterior of RER: mainly for synthesising proteins to be exported outside the cell
- Free in cytoplasm (either singly or in clusters): primarily the site of assembly of proteins to be used inside the cell
What is the structure of the centrioles?
- Consist of 2 bundles of microtubules at right angles to one another
- Made of tubulin protein subunits + are arranged to form a cylinder
What is the function of the centrioles?
- Before cell divides, spindle (made of threads of tubulin) forms from the centrioles. Chromosomes attach to middle part of spindle + motor proteins walk along tubulin threads, pulling chromosomes to opposite ends of cell
- Making cilia/undulipodia: before cilia form, centrioles multiply + line up beneath plasma membrane. Microtubules sprout outwards from each centriole, forming cilium/undulipodium
- Usually absent form cells of (higher) plants but may be presen in some unicellular green algae (e.g. Chlamydomonas)
What is the structure of the cytoskeleton?
- Network of proteins fibres within cytoplasm
- Rod-like microfilaments made of subunits of actin (a protein). They’re polymers of actin + each microfilament is about 7nm in diameter
- Intermediate filaments about 10nm in diameter
- Straight, cylindrical microtubules, made of tubulin (protein subunits) 18-30nm diameter
- Cytoskeletal motor proteins (myosins, kinesins + dyneins) are molecular motors. They’re also enzymes + have site that binds to + allows hydrolysis of ATP as their energy source