Module 2- Rods and cocci Flashcards
Characteristics of Staphyococcus
Gram positive cocci, commensal of skin and mucous membranes, aerobic
Staphylococcus virulence factors
Coagulase
Test for Staphylococcus
Rbabit plasma (positive = clot formation)
Coagulase positive Staphylococcus
S. aureus
S. hyicus
S. pseudointermedius
Staphylococcus pathogenisis
Invasion of skin or mucous membrane, inflammation, destruction of neutrophils, pus formation
Pathogens that cause mastitis
Staphylococcus
Streptococcus agalactiae
Streptococcus dysgalactiae
Nocardia
Most common staphylococcus isolate in canine pyoderma
S. pseudointermedius
Diseases caused by Staphylococccus pseudointermedius and S. aureus
pyoderma, otitis externa. Bumble foot (aureus)
Disease caused by staphylococcus hyicus
Greasy pig disease (exudative dermatitis)
Staphylococcus superantigen diseases
Staphylococal toxic shock syndrome, Staphylococcal food poisoning, Staphylococcal scalded skin syndrome
Diseases caused by coagulase negative staphylococcus
RARE. Nosocomial infections, colonization of catheters and implants
Diagnosing Staphylococcus infections
Aerobic culture, cytology/gram stain, PCR
Problems with dx Staphylococcus
Commensal of skin = false positives
Tx Staphylococcus
Treat underlying disease (Staph is opportunistic), topical antimicrobials work best
Resistant staphylococcus
Resistant to beta lactams, macrolide, aminoglycosides, tetracyclines, and sulfa.
Is Staphylococcus zoonotic?
Yes
Characteristics of Streptococcus
Gram positive cocci in chains, commensals of mucous membranes
Diseases: Streptococcus equi subsp. equi
Strangles
Strangles transmission
contaminated horses/fomites
Which age is most susceptible to strangles
yearlings, <4mo is protected from maternal antibodies
Signs of Strangles
dysphagia, lymphadenitits, moist cough, gutteral pouch empyema (complication0
Diagnosis of Strangles
Microscopic, culture, Serology, PCR
Treatment of Strangles
Most recover spontaneously, abx only effective if given before abscess formation
Is Strangles reportable
Yes
Strangles control
Strong immunity after infection, vaccine (can complicate dx)
How to detect Strangles carriers
Swab guttural pouch 10 days after recovery
How to determine clear of Strangles infection
3 negative swab culture/PCR (1x per week) before release from quarantine
Streptococcus canis infects…
Infects dogs and cats
Symptoms of Streptococcus canis infection
Skin ulceration, necrotizing fasciitis, sepsis, death
Streptococcus suis infects…
pigs
Is streptococcus sui zoonotic
yes
Symptoms of Streptococcus suis
septicemia, meningitis, pnuemonia, abortions, endocarditis
Disease caused by Streptococcus porcinus
Jowl abscess
Is Streptococcus iniae zoonotic
Yes
Symptoms of Streptococcus iniae infection
Septicemia, meningoencephalitis, epicarditis, cellulitis
Disease caused by Streptococcus pyogenes
Necrotizing fasciitis, Scarlet fever, Rheumatic fever…etc
Streptococcus pneumonia infects…
humans, guinea pigs, rodents, pets
Treatment of Streptococcus
Penicillin G
Characteristics of Enterococcus
Gram positive, opportunistic intestinal flora
Resistance in Enterococcus
Intrinsic- beta lactams, aminoglycosides, clindamycin, sulfa. Emerging- Vancomycin
Characteristics of Bacillus
Gram positive ords, aerobic, endospore-forming
Disease caused by Bacillus anthracis
anthrax
Virulence factors of Baccilus anthracis
Anti-phagocytic capsule, toxin
Diagnosis of Baccilus anthracis
McFadyean reaction stain, blue tissue and pink capsule
Three parts of Anthrax toxin
Edema factor, lethal factor, protective antigen
Pathogenesis of Baccilus anthracis
bacteria spread through the blood stream, toxin causes increased vascular permeability and cell necrosis
Transmission of Baccilus anthracis
Ingestion, inhalation, contact
Which form of Baccilus anthracis are ruminants more likely to present
Per acute septicemia (sudden death)
What form of Baccilus anthracis are humans most likely to present
Pulmonary (wool sorter’s disease), cutaneous, intestinal
Postmorteum signs of Baccilus anthracis
SPlenomegally, sudden death, bacteremia, unclotted blood
Diagnosis of Baccilus anthracis
Reportable, do not open carcas, Peripheral blood smears (Mcfadyean rxn)
Treatment of Baccilus anthracis
Do not usually treat
Control of Baccilus anthracis
Vacciante
Characteristics of Listeria
Gram positive rod, facultative intracellular, can grow at low temperatures, in intestinal tract
Pathogenesis of Listeria
Ingestion/entry through mucosal wounds, intracellular growth in blood stream, microabscesses in CNS
Control of Listeria
Good silage, food safety
Presentations of Listeria
Septicemia (visceral)- monogastric animals, abortion, necrosis of spleen and liver, focal hepatic necrosis
Neurologic- microabscesses on brain stem, circling, ruminants, unilateral facial paralysis
Dx Listeria
Speial Enrichment culture, culture brain stem/liver/spleen
Listeriosis tx
Not attempted usually, novax
Characteristics of Erysipelothrix rhusiopathiae
Gram positive rods, aerobic, tonsils and intestines, affects pigs and turkeys
Pathogenesis of Erysipelothrix rhusiopathiae
Ingested, inflammation of blood vessels
Disease caused by Erysipelothrix rhusiopathiae
Diamond skin disease (erysipelas),septicemia, endocarditis
Erysipelothrix rhusiopathiae in sheep
Post-dipping polyarthritis
Diagnosis of Erysipelothrix rhusiopathiae
aerobic culture, PCR
Treatment of Erysipelothrix rhusiopathiae
antimicrobials
Intrinsic resistance of Erysipelothrix rhusiopathiae
Vancomycin, aminoglycocides, Sulpha
COntrol of Erysipelothrix rhusiopathiae
Vaccinate, treat, isolate, cull
Characteristics of Corynebacterium
Gram positive, pleomorphic, aerobic, found in the soil, facultative intracellular
Infections caused by Corynebacterium
Pyogenic pyogranulomatous infections
Disease caused by Corynebacterium diphtheriae
Diphtheria
Disease caused by Corynebacterium peudotuberculosis
Caseous lymphadenitis
Virulence factors of Corynebacterium pseudotuberculosis
Exotoxin, phospholipase D, disease severity increases with age
Two forms of caseous lymphadenitis
Internal and external
Diagnosis of Corynebacterium pseudotuberculosis
Aerobic culture, difficult to dx internal, detect antibodies using synergistic hemolysin inhibition, paired titers
Treatment of Corynebacterium pseudotuberculosis
Not curable, lance and drain
Control of Corynebacterium pseudotuberculosis
Vaccinate, biosecurity, cull
Disease caused by Corynebacterium pseudotuberculosis in horses
Pigeon fever
Where does Corynebacterium renale colonize?
urogenital tract
Disease caused by Corynebacterium renale
Contagious bovine pyelonephritis
Corynebacterium bovis infects and causes
mice (hyperkeratosis)
Characteristics of Rhodococcus equi (hoagii)
gram positive baccili, aerobic, facultative intracellular, opporunistic in soil
Disease caused by Rhodococcus equi
Foal pneumonia
Is Rhodococcus equi zoonotic
yes
Pathogenesis of Rhodococcus equi
Nodules and inflammation in lungs, cough up pus and swallow, GI infection
Dx of Rhodococcus equi
Cytology of tracheal wash, CBC and serum, radiographs, culture of bracheal wash, PCR
Treatment of RHodococcus equi
Prolonged macrolide and rifampin
Prevention of Rhodococcus equi
Colostrum, dust control, no vax
Characteristics of Nocadia
Pleomorphic, Gram positive rod, facultative intracellular, opportunistic in soil and water, sulfur granules in exudate
Pathogenesis of Nocardia
Penetrating wound infection, pleural or peritoneal empyema
Dx of Nocardia
Direct stain, acid fast, gram, aerobic culture
Tx of Nocardia
aminoglycocide + carbapenem
Characteristics of Actinomyces
Gram positive rods, aerobic and anaerobic, sulfur granules, colonizes oral biome, source of abx
Disease caused by Actinomyces bovis
Lumpy Jaw
Dx of Actinomyces
Aerobic and Anaerobic cultures
Tx of Actinomyces
prolonged penecillin G and Iodides, surgical exceision
Characteristics of dermatophilus congolensis
Aerobic gram positive rod, branching bacteria, colonizes skin, motile zoospores
Dx of Dermatophilus congolensis
Microscopic tram-track, Culture, PCR
Disease caused by Dermatophilus congolensis
Rain scald, foot rot
Tx of Dermatophilus congolensis
systemic abx (penecillin/tetracycline)
Characteristics of Trueperella pyogenes
Gram positive rod, pleomorphic, aerobic, opportunist of GI tract
Disease caused by Trueperella pyogenes
chronic purulent infections in runinants and swine
Dx of Trueperellat pyogenes
Aerobic culture
Tx of Trueperella pyogenes
Penicillin G
Characteristics of Actinobaculum suis
Anaerobic gram positive rod, commensal of prepucial mucosa in boars
Disease caused by Actinobaculum suis
porcine cystitis, pyelonephritis, renal failure