Module 2 - Microscopy

Question 1

How many nanometers in a mm

Answer 1

1000 nm

Question 2

What is Resolution?

Answer 2

The minimum distance between two points where they can still be viewed as two separate points.

Question 3

Advantage of Light microscope

Answer 3

Cheap

Can observe living cells

Question 4

Disadvantage of light microscope

Answer 4

Low resolution

Question 5

Advantages of laser confocal Microscope

Answer 5

Produce 3D images
Can target specific points depending on dyes used
High resolution

Question 6

How does an electron microscope work?

Answer 6

Uses electron beam rather than light which have a shorter wavelength therefore improving resolution.

Question 7

Disadvantages of electron microscope

Answer 7

Contains vacuum so cells cannot be living
Expensive
All specimens have to be stained and staining can produce artefacts

Question 8

What are the two types of electron microscope?

Answer 8

Transmission and scanning

Question 9

Facts about transmission electron microscope

Answer 9

High resolution
Electron beam passes through a specimen so can see inside
Produces 2D images

Question 10

Scanning Electron microscope facts

Answer 10

Electrons scattered on surface of cell so you can only see the surface
Produces 3D
Low resolution
Specimens have to be covered in metal ions which can produce artefacts

Question 11

How do you calculate magnification

Answer 11

Image size \ Actual size

Question 12

What makes a good scientific drawing

Answer 12

Sharp pencil
Title
Labels
Over half the page 
No shading 
State magnification