Module 10

Question 1

Which end does transcription of the coding strand start?

Answer 1

the 5’ end

Question 2

What is the enzyme used for transcription of DNA strand to mRNA?

Answer 2

RNA polymerase II

Question 3

Which sub unit of the Ribosome is responsible for transcription?

Answer 3

RNA polymerase

Question 4

How do you initiate the transcription on the DNA strand

Answer 4

Promoter

Question 5

What is the use of the TATA box?

Answer 5

It is a consensus site for the promoter

Question 6

What is the use of TBP?

Answer 6

General transcription factor

Question 7

Where does the incoming NTP bind to?

Answer 7

The carboxyl group of the previous base

Question 8

What is RNA polymerase I transcripting

Answer 8

rRNA

Question 9

What is RNA polymerase III transcripting?

Answer 9

tRNA and rRNA

Question 10

What is the shape of mitochondria genome, is there introns?

Answer 10

Circular and there are no intron in eukaryotic cell

Question 11

Is genetic code of mitochondria the same the DNA in the nuclear?

Answer 11

No

Question 12

Does mitochondria create its good tRNA?

Answer 12

yes

Question 13

What causes disease in mitochondria?

Answer 13

Mutation in the mitochondrial genome or mitochondrial genes in the nuclear genome

Question 14

How do you find out the mRNA in a gene?

Answer 14

RT-PCR

Question 15

How do you remove DNA in RT-PCR?

Answer 15

DNase 1

Question 16

How do you find the mRNA in the solution?

Answer 16

using synthetic oligonucleotide (oligo-dT) primer, prime in the poly AAA tail

Question 17

What is semi-quantitative RT-PCR experiment for?

Answer 17

To find the how highly expressed genes there are in the genome of the cell

Question 18

How do you find the what proteins there are in a gene?

Answer 18

Using SDS PAGE

Question 19

What is used to recognise proteins

Answer 19

Using antibodies in a western blot