Mod 6 Quiz 1: DNA and RNA Flashcards
Transformation (bacterial transformation)
One strain of bacteria takes on characteristics of another strain by taking in pieces of DNA (plasmids)
Bacteriophage
Virus that infects bacteria
Antiparallel
Side by Side but facing in opposite directions
Complementary Nucleotides
Match up according to base pairing rules
Semi-Conservative
Description of the process that results in each molecule being 1/2 old and 1/2 new
Chargaff’s Rule
In any sample of DNA, %A = %T and %C = %G
Topoisomerase
relaxes supercoiling in front of the replication fork where strands are separating
Helicase
Unzips/separates 2 strands of DNA by breaking its bonds between bases
DNA Polymerase
Adds new complementary DNA nucleotides to each original strand; acts as a proofreader.
- needs RNA primers to be added to strands first to know where to add nucleotides - near the end of replication, RNA primers have to be replaced by DNA nucleotides
Ligase
Connects fragments of DNA to each other on the lagging strand
Leading strand
can be added to in the 5’ to 3’ direction so DNA polymerase can work continuously
Lagging strand
facing other direction; has to be added in segments because DNA polymerase can only work in 5’ to 3’ direction (discontinuous)
Okazaki Fragments
The pieces added to the lagging strand that have to be connected to each other by ligase at the end of the process
Transcription
The Process that makes all types of RNA
- tRNA and rRNA can get reused a few times
- mRNA needs to be remade fresh every time a cell needs protein
RNA Polymerase
Adds RNA nucleotides (adds RNA primers in DNA replication)