Miscellaneous II Flashcards
CD4:CD8 ratio
BM not good sample for this- rather PB is optimal
Causes of dyserythropoiesis
NK/T cell neoplasm
LGL neoplasm
Aplastic anemia
Hypocellular MDS
Should see increased blasts (otherwise, don’t flat out call it!)
Could be an evolving aplastic anemia
Monoblasts by flow
Show decreased expression of CD14 and CD36 (also CD64)
AML from MDS morphology
Often see a less packed marrow than de novo AML- still see background erythroid islands
Burkitt lymphoma in HIV
Can look plasmacytoid
Vacuoles in Burkitt
Fat- oil red O positive
Vacuoles in Erythroleukemia
Glycogen- PAS positive
c-MYC threshold IHC
50-70%
Look for Parvovirus when you see…
Anemia and erythroid hypoplasia
Percent dysplasia to call
at least 10%
Hematogones in MDS
decreased
MDS comment:
These findings (dyserythropoiesis) are non-specific, and may be seen in:
- nutritional deficiency
- meds
- infection
- autoimmune
- low grade MDS
Percent hypersegmented PMNs to call
Greater or equal to 6%
Granulocytic finding in Folate/B12 deficiency
giant band
Assessing marrow cellularity
Better to do on a bad core than on a good clot section (clot section compresses fat and falsely increased cellularity)
Left-shifted erythroids vs lymphoblasts
left-shifted erythroids= more cytoplasm
lymphoblasts= less cytoplasm
MPN with lots of mature neutrophils, lacking myelocyte bulge
Consider PMF, atypical CML, and CNL
Cat-scratch lymphadenitis
Stellate microabscess formation
if inguinal LN, cannot distinguish from lymphogranuloma venereum
Plasma cell neoplasm oral cavity
ALWAYS remember to order HHV8 and EBER
NLPHL architecture
If see PTGC but no effacement- Do NOT call NLPHL (without effacement)
Marrow s/p rituxin
can see T cells in the marrow (see Foucar paper)
Increased CD8 T cells
Can see in reactive conditions too (throws off CD4:CD8)
Burkitt
- paucity of small lymphs
- “jigsaw” appearance
- DDx: ALL, blastic mantle cell, myeloid sarc