Misc Body Fluids Flashcards
True or False:
There is no definitive test to differentiate menstrual blood from venous blood.
True
When radial gel diffusion is used for the identification of urine, it can be both
________
and
________
in nature when one takes into account both the color change and the diameter of the reaction circle generated.
qualitative, quantitative
Which urine test Heats creatinine and sodium nitroprusside with potassium ferricyanide as an oxidizing agent to form a blue product?
Salkowski test
Which urine test will form a blue precipitate
Radial gel diffusion with BTB and Urease
In which urine test urea will turn pink/red
DMAC
What urine test has Creatinine Reacts with picric acid in a solution of toluene or benzene (alkaline environment) and Forms a bright orange/red Creatinine Picrate product
Jaffe test
What urine test Uses an alkaline solution of K2HgI4. Results in a pale solution that becomes deeper yellow (or brown) in the presence of ammonia
Nessler’s Reagent
True or False:
Urea, the second largest constituent of urine after water, is specific to urine. Therefore detection/identification of urea in a forensic unknown is confirmation of the presence of urine.
False
What vaginal secretion test has Cells without glycogen stain golden yellow
and cells with glycogen stain chocolate brown
Lugol’s Iodine
What vaginal secretion test has Buccal cells stain red, Vaginal cells stain orange, and Skin cells stain magenta
Keratin staining
What vaginal secretion test has Cells without glycogen stain magenta
Periodic Acid Schiff (PAS)/Alcian Blue
In the first phase of the Edelman assay for the identification of feces, urobilinogen is oxidized with
_________ to produce urobilin (a pink-red product). In the second phase of the Edelman assay for the identification of feces, urobilin reacts with
_______
to produce a complex that fluoresces apple-green under UV light.
Mercury chloride, zinc chloride
True or False:
The term “Vaginal Secretions” is biologically correct.
False
Most common chemical tests for the identification of urine involve the detection of
________
and
_______
, both of which are components of urine that _____
unique to urine, but they _____
unique in their combination
urea, creatinine, are not, are
True or False:
Microscopy is the best identification technique for feces, as hairs and undigested food residues may be readily identifiable.
True
True or False:
It is relatively easy to obtain a full, human DNA profile of a fecal material donor due to the abundance of eukaryotic cellular material found in most feces.
False
Immunoassays, such as ELISA and RSID-Urine, use the protein _____________________________ as the basis for the identification of urine.
THG
True or False:
In normal healthy individuals urine is an abundant source of cellular material, perfect for DNA profiling.
False
List at least two characteristics or components of vomitus that can aid in its identification.
Vomitus has a low pH due to the stomach acid content and a lot of salivary alpha amylase due to the constant swallowing of saliva, which can both be used for identification of vomit.
True or False:
Urine stains can be visualized under the ALS, but they can be hard to locate because stains tend to more diffuse and fluoresce less than other body fluids.
True
True or False:
Recognition of the characteristic ammonia odor of urine when processing a crime scene or an item of evidence should be documented and can serve as one presumptive method of identification.
True
