Midterms_Enzymes

Question 1

Explains the specificity of enzyme, enzyme is pictured as conformationally rigid.

Answer 1

Emil Fischer’s Lock and Key Model (1984)

Question 2

For catalytic isomerizations.

Answer 2

Isomerases

Question 3

Occurs when an inhibitor binds with an enzyme but does not dissociate from it under reaction conditions or within time-frame.

Answer 3

irreversible inhibition

Question 4

Aka absolute specificity

Answer 4

Substrate Specificity

Question 5

Oxidizes or reduce substrate by transferring hydrogen or electrons

Answer 5

Oxido-Reductates

Question 6

Cause condensation of two molecules by splitting a phosphate bond

Answer 6

Ligases or synthetases

Question 7

characterized by the maintenance of enzyme activity even at high inhibitor concentrations

Answer 7

partial inhibition

Question 8

Structure is maintained by weak IMF

Answer 8

Koshland’s Induced Fit Hypothesis (1958)

Question 9

Hanes-Woolf Plot

Answer 9

S/v = (1/vmax)S + Km/Vmax

Question 10

Inhibitor binds to the enzyme at non active sites and reduces affinity of enzyme to substrate.

Answer 10

Noncompetitive inhibitors

Question 11

Approach of the substrate in induced fit is viewed as

Answer 11

perturbation

Question 12

Studies enzyme-catalyzed reaction rates and how changes in experimental conditions affect the reaction rates.

Answer 12

Enzyme kinetics

Question 13

Inhibitor may dissociates more easily from the enzyme after binding

Answer 13

Reversible inhibitors

Question 14

enzymes for hydrolytic reactions

Answer 14

hydrolases

Question 15

Lineweaver-Burk equation

Answer 15

1/v = km/vmax (1/s) + 1/Vmax

Question 16

Michaelis Menten Kinetics do not apply to irreversible inhibition (True or False)

Answer 16

True

Question 17

compounds bind to enzyme and reduce their activity

Answer 17

enzyme inhibitors

Question 18

removes groups and transfer them to acceptor molecules

Answer 18

Transferases

Question 19

Selectivity of the enzyme to their substrate, molecular recognition mechanism

Answer 19

Specificity

Question 20

proteins produced by living cells and acts as biological catalysts

Answer 20

Enzyme

Question 21

Essential feature of enzyme-catalyzed reactions is

Answer 21

saturation

Question 22

In the absence of _______, the enzyme will be inactive even if there are plenty of substrates.

Answer 22

Specific co-factor

Question 23

inhibitor forms a stable complex with the enzyme

Answer 23

Irreversible inhibitors

Question 24

Factors affecting enzyme activity

Answer 24

Temperature, pH, enzyme concentration and substrate concentration

Question 25

Recognition and specificity is based on ______

Answer 25

Structural complementarity

Question 26

are specific to substrates having similar bonds and similar structure

Answer 26

Bond specificity

Question 27

Eadie-Hofstee Plot

Answer 27

v = -km(v/s) + Vmax

Question 28

Enzyme activity depends on substrate concentration

Answer 28

Michaelis-Menten Theory

Question 29

is specific to a bond and groups surrounding the bonds

Answer 29

Group specificity

Question 30

enzyme can act on different substrates having similar molecular geometry and hence, here specificity is very less.

Answer 30

geometrical specificity

Question 31

Enzymes are highly specific and produce only the expected products from the given reactants or substrates (there are no side reactions). (True or False)

Answer 31

True

Question 32

remove groups from the substrate by hydrolysis to form double bonds or conversely, add groups to the double bond

Answer 32

Lyases

Question 33

specific not only to substrate but also to its optical configuration

Answer 33

Optical or stereo specificity

Question 34

Inhibitors bind to ES complex but not to enzyme

Answer 34

Uncompetitive

Question 35

refers to the catalytic ability of an enzyme to increase the rate of reaction.

Answer 35

Enzyme activity