Midterm II: Ketogenesis + breakdown (Ben) Flashcards

1
Q

What is the 1st step of ketogenesis?

(involving a β-oxidation enzyme)

Reactants?

Enzyme?

Products?

A

Condensation of two acetyl-CoA…

Reactants: 2x Acetyl-CoA

Enzyme: Thiolase (reverse rxn from β-oxidation)

Products: Acetoacetyl-CoA + CoA-SH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is the 2nd step of ketogenesis, occuring after the reversal of the thiolase reaction?

Reactants?

Enzyme?

Products?

A

Condensation…

Reactants: Acetoacetyl-CoA + Acetyl-CoA + H2O

Enzyme: HMG-CoA Synthase

Products: HMG-CoA + CoA-SH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the 3rd step of ketogenesis, after condensation via HMG-CoA synthase?

A

Cleavage…

Reactants: HMG-CoA

Enzyme: HMG-CoA Lyase

Products: Acetoacetate + Acetyl-CoA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What are the two ketone bodies which are interconverted?

By which enzyme + coenzyme?

A

Acetoacetate + β-Hydroxybutyrate

via

β-Hydroxybutyrate Dehydrogenase

( and NAD in the direction using β-OH Butyrate as a reactant)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What occurs to acetoacetate produced in ketogenesis?

A

it undergoes spontaneous decarboxylation

via acetoacetate decarboxylase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What controls the equilibrium between the two interconvertible ketone bodies?

A

Redox State

  • more specifically [NAD+]/[NADH] ratio
  • high NAD+ favors the dehydrogenation of β-hydroxybutyrate to acetoacetate
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What tissues use ketone bodies during ketosis?

A
  1. cardiac muscle
  2. skeletal muscle
  3. brain
  4. kidney (renal cortex)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is the 1st step in the oxidation of ketone bodies by extrahepatic tissues?

Reactants?

Enzyme?

Product?

A

Activation via CoA…

Reactants: Acetoacetate + Succinyl-CoA

Enzyme: β-Ketoacyl-CoA Transferase

Product: Acetoacetyl-CoA + Succinate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is the second step in oxidation of ketone bodies by extrahepatic tissues?

(resulting in a citric acid cycle intermediate)

Reactant?

Enzyme?

Product?

A

Cleavage…

Reactant: Acetoacetyl-CoA + CoA-SH

Enzyme: Thiolase

Product: 2 Acetyl-CoA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Since ketone bodies are generated starting from acetyl-CoA and then oxidized into acetyl-CoA for later use…

what is their purpose?

A

the are a transport form of acetyl groups

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

make some regulation cards

A

ok

How well did you know this?
1
Not at all
2
3
4
5
Perfectly