Microtubule motors in vesicle transport

Question 1

What cytoskeletal motors are involved in vesicle transport?

Answer 1

• Kinesins
• dyneins
• myosins

Question 2

What happens to the golgi apparatus when microtubules are disrupted?

Answer 2

The Golgi becomes dispersed

Question 3

What happens to KIF16B knockout embryos?

Answer 3

They fail to implant and the primitive ectoderm doesn’t form

Question 4

which domain of kinesin motors binds to phospholipids?

Answer 4

PX

Question 5

What domains does Kif16B contain?

Answer 5

• Kinesin motor domain
• FHA domain
• PX domain

Question 6

Why does KIF16B knockout produce unviable embryos?

Answer 6

• Defective in FGF signalling
• Defective in biosynthetic transport of FGFR to plasma membrane

Question 7

What signalling controls ectoderm differentiation?

Answer 7

FGF signalling

Question 8

How do KIF16B knockouts disrupt FGF signalling?

Answer 8

FGFR2 does not traffic to plasm membrane

Question 9

What does the PX domain especially like to bind?

Answer 9

PI3P

Question 10

How is KIF16B an example of coincidence detection?

Answer 10

Targeting of KIF16B to early endosomes requires both PI3P and Rab14 binding

Question 11

What protein does KIF16B also interact with?

Answer 11

Rab14

Question 12

What do Q to L Rab mutants do?

Answer 12

Bind to GTP but are unable to hydrolyse it

Question 13

What do S to N Rab mutants do?

Answer 13

Prevent residue binding, mimicks GDP bound state

Question 14

What is the effect of using dominant negative Rab14 on embryonic development?

Answer 14

• Disrupts primitive ectoderm as it prevents delivery of FGFR to plasma membrane