Microsopy- Calculation

Question 1

What is the formula for magnification?

Answer 1

Magnification = Image Size ÷ Actual Size

Question 2

What is the formula for magnification?

Answer 2

Magnification = Image Size ÷ Actual Size

Question 3

How do you calculate the actual size of a specimen?

Answer 3

Actual Size = Image Size ÷ Magnification

Question 4

How do you calculate the image size of a specimen?

Answer 4

Image Size = Actual Size × Magnification

Question 5

What units should you use for microscopy calculations?

Answer 5

All measurements should be in the same unit.

For example:
• 1 mm = 1,000 µm
• 1 cm = 10,000 µm

Question 6

Example Calculation 1: A cell appears 8 mm wide under a microscope with ×200 magnification. What is its actual size?

Answer 6

Actual Size = Image Size ÷ Magnification = 8 mm ÷ 200 = 0.04 mm (or 40 µm).

Question 7

Example Calculation 2: An organelle is 0.05 mm in actual size and is magnified ×400. What is the image size?

Answer 7

Image Size = Actual Size × Magnification = 0.05 mm × 400 = 20 mm.

Question 8

How do you estimate the size of a cell using the field of view (FOV)?

Answer 8

• Measure the FOV diameter under the microscope (e.g., 0.5 mm).
• Count how many cells fit across the diameter.
• Divide the FOV diameter by the number of cells.

Example: If 10 cells fit across a 0.5 mm FOV: Cell size = 0.5 mm ÷ 10 = 0.05 mm (50 µm).

Question 9

What is the formula for magnification?

Answer 9

Magnification = Image Size ÷ Actual Size

Question 10

How do you calculate the actual size of a specimen?

Answer 10

Actual Size = Image Size ÷ Magnification

Question 11

How do you calculate the image size of a specimen?

Answer 11

Image Size = Actual Size × Magnification

Question 12

What units should you use for microscopy calculations?

Answer 12

All measurements should be in the same unit.

For example:
• 1 mm = 1,000 µm
• 1 cm = 10,000 µm

Question 13

Example 1: A cell appears 8 mm wide under a microscope with ×200 magnification. What is its actual size?

Answer 13

Actual Size = Image Size ÷ Magnification
= 8 mm ÷ 200
= 0.04 mm (or 40 µm).

Question 14

Example 2: An organelle is 0.05 mm in actual size and is magnified ×400. What is the image size?

Answer 14

Image Size = Actual Size × Magnification
= 0.05 mm × 400
= 20 mm.

Question 15

Example 3: The field of view (FOV) diameter is 0.5 mm, and 10 cells fit across the FOV. How do you estimate the size of each cell?

Answer 15

Cell Size = FOV Diameter ÷ Number of cells
= 0.5 mm ÷ 10
= 0.05 mm (or 50 µm).