Microscopy

Question 1

What type of microscope uses visible light to illuminate specimens?

Answer 1

Light microscope

Question 2

True or False: Electron microscopes can achieve higher magnifications than light microscopes.

Answer 2

True

Question 3

Fill in the blank: The maximum magnification of a light microscope is approximately ______.

Answer 3

1000x

Question 4

What is the primary advantage of using an electron microscope over a light microscope?

Answer 4

Higher resolution and magnification

Question 5

Which type of microscope is typically used for observing live specimens?

Answer 5

Light microscope

Question 6

What type of electron microscope uses a beam of electrons transmitted through a specimen?

Answer 6

Transmission electron microscope (TEM)

Question 7

Select the correct option: Which microscope is best for viewing the surface of a specimen? A) Light microscope B) Scanning electron microscope C) Transmission electron microscope

Answer 7

B) Scanning electron microscope

Question 8

Mention some disadvantages of electron microscopy?

Answer 8

Specimens must be fixed and cannot be alive
It’s expensive to purchase
It’s not mobile

Question 9

True or False: Light microscopes can provide 3D images of specimens.

Answer 9

False

Question 10

Fill in the blank: The resolution of a light microscope is limited to about ______ nanometers.

Answer 10

200

Question 11

What type of lenses do light microscopes use?

Answer 11

Glass lenses

Question 12

What is the main source of illumination in a light microscope?

Answer 12

Visible light

Question 13

True or False: Electron microscopes require a vacuum environment to operate.

Answer 13

True

Question 14

What is the primary use of light microscopes in laboratories?

Answer 14

To observe and analyze live cells and tissues

Question 15

Fill in the blank: Electron microscopes can achieve magnifications up to ______.

Answer 15

2,000,000x

Question 16

What type of specimen preparation is required for electron microscopy?

Answer 16

Specimen must be fixed, dehydrated, and often coated with a conductive layer

Question 17

What is the difference in the way light and electron microscopes produce images?

Answer 17

Light microscopes use light rays, while electron microscopes use electron beams.

Question 18

Select the correct option: Which microscope provides better depth of field? A) Light microscope B) Electron microscope

Answer 18

A) Light microscope

Question 19

What type of microscopy allows for the observation of live cells in real-time?

Answer 19

Live-cell imaging using light microscopy

Question 20

True or False: Electron microscopes can provide detailed internal structures of cells.

Answer 20

True

Question 21

What is the role of the condenser lens in a light microscope?

Answer 21

To focus light onto the specimen

Question 22

Fill in the blank: The primary detector used in electron microscopy is called a ______.

Answer 22

CCD camera or electron detector

Question 23

What is a common application of scanning electron microscopy?

Answer 23

Surface topography analysis

Question 24

What is the typical resolution of a transmission electron microscope?

Answer 24

Less than 1 nanometer

Question 25

Select the correct option: Which microscope is NOT suitable for observing living organisms? A) Light microscope B) Scanning electron microscope C) Both A and B

Answer 25

B) Scanning electron microscope

Question 26

What is one reason why light microscopes are more accessible than electron microscopes?

Answer 26

They are less expensive and easier to operate.