Microsocpes Flashcards
What is the maximum mag and res for a light microscope
x1500 mag
200nm
What is the maximum mag and res for tem
x500 000 mag
0.2 nm
what is the maximum mag and res
x100 000 mag
10nm
What does SEM stand for
Scanning electron microscope
What does TEM stand for
Transmission electron microscope
Calculation for image size
image size= mag x actual size
Features of light microscopes
Cheap
Live specimen
Coloured image
Low mag
Low res
Inverted image
3 lenses
Features of electron microscopes
Expensive
High mag
High res
Dead specimen
Black and White image
Shows subcellular organelles
Magnets instead of lenses
Features of SEM
outside layer
3D image
Features of TEM
inside image
2D image
the denser the area the more electrons absorbed which results in a darker image
Features of laser confocal microscope
Laser fired at dead specimen
3D image
different depths are shown
What are the 3 lenses
Objective
Condenser
Ocular
What criteria does a cell drawing have
Title
Mag labelled
Straight lines
No arrow heads
Pencil
No crossing lines
No shading
Over 50% of the space provided
Correct proportions
Why does the specimen need to be dead for an electron microscope
Because it is viewed in a vacuum
Give 2 similarties of light and electron microscopes
2D images
can use dead specimen