Microsocpes Flashcards

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1
Q

What is the maximum mag and res for a light microscope

A

x1500 mag
200nm

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2
Q

What is the maximum mag and res for tem

A

x500 000 mag
0.2 nm

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3
Q

what is the maximum mag and res

A

x100 000 mag
10nm

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4
Q

What does SEM stand for

A

Scanning electron microscope

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5
Q

What does TEM stand for

A

Transmission electron microscope

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6
Q

Calculation for image size

A

image size= mag x actual size

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7
Q

Features of light microscopes

A

Cheap
Live specimen
Coloured image
Low mag
Low res
Inverted image
3 lenses

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8
Q

Features of electron microscopes

A

Expensive
High mag
High res
Dead specimen
Black and White image
Shows subcellular organelles
Magnets instead of lenses

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9
Q

Features of SEM

A

outside layer
3D image

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10
Q

Features of TEM

A

inside image
2D image
the denser the area the more electrons absorbed which results in a darker image

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11
Q

Features of laser confocal microscope

A

Laser fired at dead specimen
3D image
different depths are shown

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12
Q

What are the 3 lenses

A

Objective
Condenser
Ocular

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13
Q

What criteria does a cell drawing have

A

Title
Mag labelled
Straight lines
No arrow heads
Pencil
No crossing lines
No shading
Over 50% of the space provided
Correct proportions

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14
Q

Why does the specimen need to be dead for an electron microscope

A

Because it is viewed in a vacuum

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15
Q

Give 2 similarties of light and electron microscopes

A

2D images
can use dead specimen

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16
Q

Some prokaryotes have an extra layer around the cell wall known as a ________

A

capsule

17
Q
A