Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Histology > Microscopy Concepts > Flashcards

Microscopy Concepts Flashcards

1
Q

What are the 6 Steps to Specimen Preparation?

A 
Fixation
Dehydration
Infiltration & Embedding
Sectioning
Staining
Microscopy
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What are the primary reasons for Fixation?

A

Prevent Autolysis
Preserve Cell Structure
Minimize redistribution of proteins and organelles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What are the 2 Protein fixatives

A

Formaldehyde

Glutaraldehyde

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is the Lipid Fixative?

A

Osmium tetroxide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is Artifact?

A

A feature of a tissue section that is due to processing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What are 2 stains used for Electron Microscopy?

A

Lead citrate

Uranyl acetate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the name of the blue stain for light microscopy?

A

Hematoxlyin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is the name of the pink-orange stain for light microscopy?

A

Eosin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is the name of the stain designated by ā€œH&Eā€?

A

Hematoxylin & Eosin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What stain would you use if you wanted to detect carbohydrates?

A

Periodic acid-Schiff (PAS)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What stain would you use to detect lipids?

A

Osmium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is microscopic resolution?

A

The smallest distance of separation at which two objects can still be distinguished

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is resolution determined by?

A

wavelength of illumination source

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the resolution of a light microscope?

A

200 nm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is the resolution of an EM?

A

1 nm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is the max magnification of a LM?

A

1000

17
Q

What is the max magnification of an EM?

A

500K

18
Q

What is the approximate size of regions or portions of organs?

A

mm

19
Q

What is the approximate size of cells and large organelles?

A

um

20
Q

What is the approximate size of cell membranes and filaments?

A

nm

21
Q

What is the approximate size of macromolecules?

A

.1 nm (Angstrom unit)

22
Q

What is formaldehyde in solution called?

A

Formalin

23
Q

To what types of charged components does hematoxylin bind to?

A

Negative

24
Q

What color is hematoxlyin?

A

Blue/Purple

25
Q

Why does the nucleus and RER absorb so much hematoxylin?

A

They have a lot of nucleic acids with negative charges associated

26
Q

What parts of cells are eosiniphilic?

A

Cytoplasm, Collagen fibers

Histology (11 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions