microscopy and quantitation of bacteria Flashcards

(63 cards)

1
Q

What are the three different types of microscope used in bacteriology?

A
  • Bright field microscopy
  • Phase contrast microscopy
  • Flourescence microscopy
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2
Q

Define what is a bright field microscopy

A

It examines the detail of an microorganism by the transmitted light

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3
Q

It examines the detail of an microorganism by the transmitted light

A

Bright field microscopy

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4
Q

Define what is a phase contrast microscopy

A

It uses contrast enhancement technique allows examination of unstained specimen

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5
Q

It uses contrast enhancement technique allows examination of unstained specimen

A

Phase contrast microscopy

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6
Q

Define what is a fluorescence microscopy

A

The specimen are stained with fluorochrome or fluorescent dye

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7
Q

The specimen are stained with fluorochrome or fluorescent dye

A

Fluorescence microscopy

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8
Q

It is used in cleaning the objective lenses and ocular lens

A

Lens paper

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9
Q

T OR F

The mechanical stage must be on the lowest part before/after using it

A

True

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10
Q

It is used to change the objective lenses

A

Revolving nosepiece

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11
Q

T OR F

Objective lenses must be on the lowest magnification before/after using it

A

True

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12
Q

Where do we hold our hands when carrying the microscope?

A

Arms and Base

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13
Q

Minimum number in turning the rheostat when turning on the microscope

A

1

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14
Q

First objective lens to use when we are starting to focus the specimen

A

Scanner

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15
Q

What is the knob to use when using the Scanner and LPO?

A

Coarse Adjustment Knob

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16
Q

What is the knob to use when using the HPO and OIO?

A

Fine adjustment knob

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17
Q

It is added on the slide before using the OIO

A

Cedar wood (oil)

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18
Q

It is the ratio of the apparent size of an object as seen through the microscope & the actual size of the object

A

Magnification

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19
Q

The ability of the lens to clearly separate or distinguish two points of two lines individually in the image.

A

Resolution/Resolving Power

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20
Q

It is determined by the shortest wavelength of visible light & maximum numerical aperture

A

Resolution/Resolving Power

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21
Q

A measurement of the ability of the condenser and the objective lens to gather light.

A

Numerical Aperture

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22
Q

Thickness of the object that maybe seen at one time under focus.

A

Focal length

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23
Q

Distance between the front lens of the objective lens & the top of the cover glass when the specimen is in focus.

A

Working distance

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24
Q

Refers to quality of the objectives & eyepiece where practically no change in focus has to be made when objective is substituted for another

A

Parfocal

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25
Bending of light rays away from the objective lens when light passes from the glass of the microscope slide to the air.
Refractive Index
26
Resolving power of LPO
1.34 um
27
Resolving power of HPO
0.52 um
28
Resolving power of OIO
0.26 um
29
What type of LENS has the higher resolving power? a. OIO b. LPO c. HPO
B. LPO
30
Numerical aperture of LPO
0.25 NA
31
Numerical aperture of HPO
0.65 NA
32
Numerical aperture of OIO
1.30 NA
33
What type of LENS has the higher numerical aperture? a. LPO b. HPO c. OIO
C. OIO
34
Magnifying power of Scanner
4x
35
Magnifying power of LPO
10x
36
Magnifying power of HPO
40x
37
Magnifying power of OIO
100x
38
What type of LENS has the higher magnifying power? a. Scanner b. HPO c. OIO d. LPO
C. OIO
39
What type of LENS has the lower magnifying power? a. Scanner b. HPO c. OIO d. LPO
A. Scanner
40
Field of view diameter of LPO
2.00 mm
41
Field of view diameter of HPO
0.40 mm
42
Field of view diameter of OIO
0.20 mm
43
What type of LENS has the higher field of view diameter? a. HPO b. OIO c. LPO
C. LPO
44
Working distance of LPO
7.20 mm
45
Working distance of HPO
0.60 mm
46
Working distance of OIO
0.20 mm
47
What type of LENS has the higher working distance? a. Scanner b. HPO c. OIO d. LPO
D. LPO
48
Where do we get the formula of TOTAL magnification?
From the **Eyepiece** and **Objective lens**
49
Formula of Total magnification
Eyepiece x Objective
50
Eyepiece magnification
10x
51
Total magnification of eyepiece and scanner
40x
52
Total magnification of eyepiece and LPO
100x
53
Total magnification of eyepiece and HPO
400x
54
Total magnification of eyepiece and OIO
1000x
55
It refers to the ratio of the size of the image to the size of the object.
Magnification
56
What values do we use in computing the magnification?
The value of: - **Image size** - **Actual size**
57
Unit used for specimen size under the microscope
UNIT: **Micrometers**
58
Formula for computing **magnification**
Magnification = Image size (um) / Actual size (um)
59
1 mm is equivalent to what in micrometers (um)?
1000 um
60
1000 um is equivalent to what in millimeter?
1 mm
61
Compute for the magnification Image size: 20 mm Actual size: 100 um
Magnification: 20,000 um / 100um = **200x** 20 mm > 20,000 um
62
Formula for the **microorganism size**
Actual size (um): Image size / Magnification
63
Compute for the microorganism size: OIO magnification: 100x Image size measured: 1000um
Actual size: 1000 um / 100 = **10 um**