microscopy and quantitation of bacteria Flashcards
(63 cards)
What are the three different types of microscope used in bacteriology?
- Bright field microscopy
- Phase contrast microscopy
- Flourescence microscopy
Define what is a bright field microscopy
It examines the detail of an microorganism by the transmitted light
It examines the detail of an microorganism by the transmitted light
Bright field microscopy
Define what is a phase contrast microscopy
It uses contrast enhancement technique allows examination of unstained specimen
It uses contrast enhancement technique allows examination of unstained specimen
Phase contrast microscopy
Define what is a fluorescence microscopy
The specimen are stained with fluorochrome or fluorescent dye
The specimen are stained with fluorochrome or fluorescent dye
Fluorescence microscopy
It is used in cleaning the objective lenses and ocular lens
Lens paper
T OR F
The mechanical stage must be on the lowest part before/after using it
True
It is used to change the objective lenses
Revolving nosepiece
T OR F
Objective lenses must be on the lowest magnification before/after using it
True
Where do we hold our hands when carrying the microscope?
Arms and Base
Minimum number in turning the rheostat when turning on the microscope
1
First objective lens to use when we are starting to focus the specimen
Scanner
What is the knob to use when using the Scanner and LPO?
Coarse Adjustment Knob
What is the knob to use when using the HPO and OIO?
Fine adjustment knob
It is added on the slide before using the OIO
Cedar wood (oil)
It is the ratio of the apparent size of an object as seen through the microscope & the actual size of the object
Magnification
The ability of the lens to clearly separate or distinguish two points of two lines individually in the image.
Resolution/Resolving Power
It is determined by the shortest wavelength of visible light & maximum numerical aperture
Resolution/Resolving Power
A measurement of the ability of the condenser and the objective lens to gather light.
Numerical Aperture
Thickness of the object that maybe seen at one time under focus.
Focal length
Distance between the front lens of the objective lens & the top of the cover glass when the specimen is in focus.
Working distance
Refers to quality of the objectives & eyepiece where practically no change in focus has to be made when objective is substituted for another
Parfocal
