Microscopy Flashcards
What are the 2 different types of microscopes ?
Light microscope and electron microscope
What is a light microscope ?
A microscope that uses light and lenses to form an image and magnify it
What is an electron microscope ?
A microscope that uses electrons to form an image
What is the difference between a light and electron microscope ?
Electron microscopes have higher magnification and resolution, therefore they allow use to see smaller things in more detail
How do you calculate magnification ?
magnification = image size/real size
How do you prepare an onion microscope slide ?
1) cut up onion and use tweezers to peel of some epidermal tissue from the layers
2) place the the layer into the slide
3) Use a drop of iodine solution to stain the onion
4) Place the cover slip over the onion trying to avoid as many air bubbles as possible