Microscopy Flashcards
Which two types of microscopes have we studied?
light microscopes and electron microscopes
What is magnification?
How large an image is, compared to the size of the original item
What is resolution?
How easy it is to distinguish between two points in an image; how clear it is
Which type of microscope has a higher magnification?
Electron microscopes
Which type of microscope has a higher resolution?
Electron microscope
Why is a high magnification and resolution important when viewing cells?
Allows you to see extra organelles or structures
What are some disadvantages of electron microscopes?
They are expensive, they take up lots of space, you need special training in order to use them
How are plant and animal cells prepared to make a slide?
take a thin layer of cells and put it on the slide. Add a drop of stain (iodine for plant, methyl blue for animal). Put a cover slip on and blot away excess liquid.
How should a light microscope be used to view a pre-prepared slide?
The slide is placed on the stage and held in place by clips. The stage is put up to the highest point. View the slide with the lowest magnification lens. Look through the eyepiece. Use the focusing wheel to lower the stage until the image is in focus. Use a higher magnification lens if needed.
How many micrometers are in a milimetre?
1000
How many nanometres are in a milimetre?
1,000,000
How many metres are in a milimetre?
0.001
What is the number 127,000,000 in standard form?
1.27 x 10^8
What is the number 0.00000345 in standard form?
3.45 x 10^-6
How is magnification calculated?
Magnification = image size / actual size
