Microscopy Flashcards
1
Q
Resolution
A
The ability to see to objects that are close together as two separate objects.
2
Q
Light Microscope
A
Mag = x1500 Res = 200nm - Live specimens - Cheap - Low skill level
3
Q
Transmitting Electron Microscope
A
Mag = x500 000
Res = 0.1-0.4nm
Electrons pass specimen and 2D image is formed
- Expensive
- Lens needs to be clean from any dirt/dust
4
Q
Scanning Electron Microscope
A
Mag = x100 000 Res = 0.1-0.4nm Electrons bounce off specimen. 3D surface detail observed. - Expensive - Lens has to be clean
5
Q
Magnification
A
The number of times bigger an image is compared to the object.