MICROSCOPY

Question 1

RESOLUTION

Answer 1

The abilty to see two objects that are close together as separate objects.
To see detail.

Question 2

What is the magnification of a light microscope?

Answer 2

x1500

Question 3

What is the magnification of a scanning electron microscope?

Answer 3

x100,000

Question 4

What is the magnification of a transmission electron microscope?

Answer 4

x500,000

Question 5

What is the resolution of a light microscope?

Answer 5

200nm

Question 6

What is the resolution of a scanning electron microscope?

Answer 6

0.1nm-0.4nm

Question 7

What is the resolution of a transmission electron microscope?

Answer 7

0.1nm-0.4nm

Question 8

Is Transmission 2D or 3D?

Answer 8

2D

Question 9

Is scanning 2D or 3D?

Answer 9

3D

Question 10

MAGNIFICATION

Answer 10

The number of times larger an image is compared to the object.

Question 11

Explain why staining is important when using a microscope.

Answer 11

• Features become visible and can be seen. (Example)
• Increases contrast.
• Allows identification between different types of organelles. (Example)

Question 12

What are the advantages of using a light microscope?

Answer 12

• Cheap
• Can view live specimens
• Does not require a high degree of skill and training to use

Question 13

What are the advantages of using an electron microscope?

Answer 13

• High resolution of 0.1nm-0.4nm

- High magnification of up to x500,000

Question 14

What are the disadvantages of using a light microscope?

Answer 14

• Low resolution of 200nm

- Low magnification of x1500

Question 15

What are the disadvantages of using an electron microscope?

Answer 15

• Expensive
• Can only view dead specimens
• Requires a high degree of skill and training to use

Question 16

What are all the units in descending order.

Answer 16

Metre > Millimetre > Micrometer > Nanometre